A mouse model of peripheral nerve injury induced by Japanese encephalitis virus.
Japanese encephalitis virus (JEV) is the most important cause of acute encephalitis in Eastern/Southern Asia. Infection with this virus also induces peripheral nerve injury. However, the disease pathogenesis is still not completely understood. Reliable animal models are needed to investigate the mol...
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ftdoajarticles:oai:doaj.org/article:cb88386182ce42448d64663d746657e2 2023-05-15T15:08:11+02:00 A mouse model of peripheral nerve injury induced by Japanese encephalitis virus. Xiaoli Wang Guowei Wang Huan Yang Shihong Fu Ying He Fan Li Huanyu Wang Zhenhai Wang 2022-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010961 https://doaj.org/article/cb88386182ce42448d64663d746657e2 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010961 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010961 https://doaj.org/article/cb88386182ce42448d64663d746657e2 PLoS Neglected Tropical Diseases, Vol 16, Iss 11, p e0010961 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010961 2022-12-30T19:28:45Z Japanese encephalitis virus (JEV) is the most important cause of acute encephalitis in Eastern/Southern Asia. Infection with this virus also induces peripheral nerve injury. However, the disease pathogenesis is still not completely understood. Reliable animal models are needed to investigate the molecular pathogenesis of this condition. We studied the effect of Japanese encephalitis virus infection in C57BL/6 mice after a subcutaneous challenge. Limb paralysis was determined in mice using behavioral tests, including a viral paralysis scale and the hanging wire test, as well as by changes in body weight. Nerve conduction velocity and electromyography testing indicated the presence of demyelinating neuropathy of the sciatic nerve. Pathological changes in neural tissues were examined by immunofluorescence and transmission electron microscopy, which confirmed that the predominant pathologic change was demyelination. Although Western blots confirmed the presence of the virus in neural tissue, additional studies demonstrated that an immune-induced inflammatory response resulted in severe never injury. Immunofluorescence confirmed the presence of Japanese encephalitis virus in the brains of infected mice, and an inflammatory reaction was observed with hematoxylin-eosin staining as well. However, these observations were inconsistent at the time of paralysis onset. In summary, our results demonstrated that Japanese encephalitis virus infection could cause inflammatory demyelination of the peripheral nervous system in C57BL/6 mice. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 11 e0010961 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xiaoli Wang Guowei Wang Huan Yang Shihong Fu Ying He Fan Li Huanyu Wang Zhenhai Wang A mouse model of peripheral nerve injury induced by Japanese encephalitis virus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Japanese encephalitis virus (JEV) is the most important cause of acute encephalitis in Eastern/Southern Asia. Infection with this virus also induces peripheral nerve injury. However, the disease pathogenesis is still not completely understood. Reliable animal models are needed to investigate the molecular pathogenesis of this condition. We studied the effect of Japanese encephalitis virus infection in C57BL/6 mice after a subcutaneous challenge. Limb paralysis was determined in mice using behavioral tests, including a viral paralysis scale and the hanging wire test, as well as by changes in body weight. Nerve conduction velocity and electromyography testing indicated the presence of demyelinating neuropathy of the sciatic nerve. Pathological changes in neural tissues were examined by immunofluorescence and transmission electron microscopy, which confirmed that the predominant pathologic change was demyelination. Although Western blots confirmed the presence of the virus in neural tissue, additional studies demonstrated that an immune-induced inflammatory response resulted in severe never injury. Immunofluorescence confirmed the presence of Japanese encephalitis virus in the brains of infected mice, and an inflammatory reaction was observed with hematoxylin-eosin staining as well. However, these observations were inconsistent at the time of paralysis onset. In summary, our results demonstrated that Japanese encephalitis virus infection could cause inflammatory demyelination of the peripheral nervous system in C57BL/6 mice. |
format |
Article in Journal/Newspaper |
author |
Xiaoli Wang Guowei Wang Huan Yang Shihong Fu Ying He Fan Li Huanyu Wang Zhenhai Wang |
author_facet |
Xiaoli Wang Guowei Wang Huan Yang Shihong Fu Ying He Fan Li Huanyu Wang Zhenhai Wang |
author_sort |
Xiaoli Wang |
title |
A mouse model of peripheral nerve injury induced by Japanese encephalitis virus. |
title_short |
A mouse model of peripheral nerve injury induced by Japanese encephalitis virus. |
title_full |
A mouse model of peripheral nerve injury induced by Japanese encephalitis virus. |
title_fullStr |
A mouse model of peripheral nerve injury induced by Japanese encephalitis virus. |
title_full_unstemmed |
A mouse model of peripheral nerve injury induced by Japanese encephalitis virus. |
title_sort |
mouse model of peripheral nerve injury induced by japanese encephalitis virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010961 https://doaj.org/article/cb88386182ce42448d64663d746657e2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 11, p e0010961 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010961 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010961 https://doaj.org/article/cb88386182ce42448d64663d746657e2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010961 |
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PLOS Neglected Tropical Diseases |
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16 |
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11 |
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e0010961 |
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