Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers
Objective:: To investigate the potential role of human cytomegalovirus lower matrix phosphoprotein 65 (HCMV-pp65) in murine systemic lupus erythematosus (SLE). Methods:: The prokaryotic plasmid pET-28b-pp65 was constructed to express the HCMV-pp65 protein. BXSB mice and C57BL/6 mice were inoculated...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:cb6b180bd92f4d81bb391db3492375a2 2023-05-15T15:11:27+02:00 Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers Yuan Zhang Ting-Ting Jia Yang Pan Wen-Li Li Yu Sun Jin-Ming Li Lu-Nan Wang 2015-02-01T00:00:00Z https://doi.org/10.1016/S2221-1691(15)30158-1 https://doaj.org/article/cb6b180bd92f4d81bb391db3492375a2 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115301581 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(15)30158-1 https://doaj.org/article/cb6b180bd92f4d81bb391db3492375a2 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 2, Pp 138-145 (2015) Systemic lupus erythematosus Autoantibody Human cytomegalovirus Lower matrix phosphoprotein 65 Cytokine MicroRNA Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/S2221-1691(15)30158-1 2022-12-31T02:21:49Z Objective:: To investigate the potential role of human cytomegalovirus lower matrix phosphoprotein 65 (HCMV-pp65) in murine systemic lupus erythematosus (SLE). Methods:: The prokaryotic plasmid pET-28b-pp65 was constructed to express the HCMV-pp65 protein. BXSB mice and C57BL/6 mice were inoculated with pp65 eukaryotic plasmid pcDNA3.0-pp65 intramuscularly 5 times at 2-week intervals, and then the blood of the mice was subsequently collected via the retro-orbital vein. Indirect ELISAs were used to evaluate the concentration of anti-pp65 immunoglobulin G, anti-double-stranded DNA and antinuclear antibodies. Interleukin-1β and tumor necrosis factor-α were also determined by competitive ELISA. At the same time, 3 major SLE-related circulating microRNAs were examined by quantitative RT-PCR. Results:: The early production of autoantibodies was observed in pp65-immunized male BXSB as well as C57BL/6 mice. Overexpression of interleukin-1β and tumor necrosis factor-α were detected in pp65-immunized male BXSB mice. Quantitative RT-PCR analyses showed that three SLE related microRNAs (microRNA-126, microRNA-125a, and microRNA-146a) were down-regulated in peripheral blood mononuclear cells of pp65-immunized mice. Conclusions:: Our findings indicate that HCMV-pp65 immunization strongly triggers the development and progression of SLE-like disease in both BXSB and C57BL/6 mice, which indicates that the immune responses induced by HCMV-pp65 may be involved in the development of SLE. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 2 138 145 |
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Systemic lupus erythematosus Autoantibody Human cytomegalovirus Lower matrix phosphoprotein 65 Cytokine MicroRNA Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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Systemic lupus erythematosus Autoantibody Human cytomegalovirus Lower matrix phosphoprotein 65 Cytokine MicroRNA Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Yuan Zhang Ting-Ting Jia Yang Pan Wen-Li Li Yu Sun Jin-Ming Li Lu-Nan Wang Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
topic_facet |
Systemic lupus erythematosus Autoantibody Human cytomegalovirus Lower matrix phosphoprotein 65 Cytokine MicroRNA Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective:: To investigate the potential role of human cytomegalovirus lower matrix phosphoprotein 65 (HCMV-pp65) in murine systemic lupus erythematosus (SLE). Methods:: The prokaryotic plasmid pET-28b-pp65 was constructed to express the HCMV-pp65 protein. BXSB mice and C57BL/6 mice were inoculated with pp65 eukaryotic plasmid pcDNA3.0-pp65 intramuscularly 5 times at 2-week intervals, and then the blood of the mice was subsequently collected via the retro-orbital vein. Indirect ELISAs were used to evaluate the concentration of anti-pp65 immunoglobulin G, anti-double-stranded DNA and antinuclear antibodies. Interleukin-1β and tumor necrosis factor-α were also determined by competitive ELISA. At the same time, 3 major SLE-related circulating microRNAs were examined by quantitative RT-PCR. Results:: The early production of autoantibodies was observed in pp65-immunized male BXSB as well as C57BL/6 mice. Overexpression of interleukin-1β and tumor necrosis factor-α were detected in pp65-immunized male BXSB mice. Quantitative RT-PCR analyses showed that three SLE related microRNAs (microRNA-126, microRNA-125a, and microRNA-146a) were down-regulated in peripheral blood mononuclear cells of pp65-immunized mice. Conclusions:: Our findings indicate that HCMV-pp65 immunization strongly triggers the development and progression of SLE-like disease in both BXSB and C57BL/6 mice, which indicates that the immune responses induced by HCMV-pp65 may be involved in the development of SLE. |
format |
Article in Journal/Newspaper |
author |
Yuan Zhang Ting-Ting Jia Yang Pan Wen-Li Li Yu Sun Jin-Ming Li Lu-Nan Wang |
author_facet |
Yuan Zhang Ting-Ting Jia Yang Pan Wen-Li Li Yu Sun Jin-Ming Li Lu-Nan Wang |
author_sort |
Yuan Zhang |
title |
Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
title_short |
Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
title_full |
Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
title_fullStr |
Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
title_full_unstemmed |
Identification of human cytomegalovirus phosphoprotein 65 in C57BL/6 and BXSB mice as a potential trigger of systemic lupus erythematosus related serum markers |
title_sort |
identification of human cytomegalovirus phosphoprotein 65 in c57bl/6 and bxsb mice as a potential trigger of systemic lupus erythematosus related serum markers |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/S2221-1691(15)30158-1 https://doaj.org/article/cb6b180bd92f4d81bb391db3492375a2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 2, Pp 138-145 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115301581 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(15)30158-1 https://doaj.org/article/cb6b180bd92f4d81bb391db3492375a2 |
op_doi |
https://doi.org/10.1016/S2221-1691(15)30158-1 |
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Asian Pacific Journal of Tropical Biomedicine |
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5 |
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2 |
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138 |
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145 |
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1766342304535150592 |