High pathogenicity avian influenza A (H5N1) clade 2.3.4.4b virus infection in a captive Tibetan black bear (Ursus thibetanus): investigations based on paraffin-embedded tissues, France, 2022
ABSTRACTHigh pathogenicity avian influenza viruses (HPAIVs) H5Nx of clade 2.3.4.4b have been circulating increasingly in both wild and domestic birds in recent years. In turn, this has led to an increase in the number of spillover events affecting mammals. In November 2022, an HPAIV H5N1 caused an o...
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2024
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ftdoajarticles:oai:doaj.org/article:cb60127ebead4a26aa76bb13de5274a1 2024-09-15T18:41:36+00:00 High pathogenicity avian influenza A (H5N1) clade 2.3.4.4b virus infection in a captive Tibetan black bear (Ursus thibetanus): investigations based on paraffin-embedded tissues, France, 2022 Pierre Bessière Nicolas Gaide Guillaume Croville Manuela Crispo Maxime Fusade-Boyer Yanad Abou Monsef Malorie Dirat Marielle Beltrame Philippine Dendauw Karin Lemberger Jean-Luc Guérin Guillaume Le Loc'h 2024-03-01T00:00:00Z https://doi.org/10.1128/spectrum.03736-23 https://doaj.org/article/cb60127ebead4a26aa76bb13de5274a1 EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/spectrum.03736-23 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.03736-23 2165-0497 https://doaj.org/article/cb60127ebead4a26aa76bb13de5274a1 Microbiology Spectrum, Vol 12, Iss 3 (2024) influenza zoonotic infections epidemiology Microbiology QR1-502 article 2024 ftdoajarticles https://doi.org/10.1128/spectrum.03736-23 2024-08-05T17:49:53Z ABSTRACTHigh pathogenicity avian influenza viruses (HPAIVs) H5Nx of clade 2.3.4.4b have been circulating increasingly in both wild and domestic birds in recent years. In turn, this has led to an increase in the number of spillover events affecting mammals. In November 2022, an HPAIV H5N1 caused an outbreak in a zoological park in the south of France, resulting in the death of a Tibetan black bear (Ursus thibetanus) and several captive and wild bird species. We detected the virus in various tissues of the bear and a wild black-headed gull (Chroicocephalus ridibundus) found dead in its enclosure using histopathology, two different in situ detection techniques, and next-generation sequencing, all performed on formalin-fixed paraffin-embedded tissues. Phylogenetic analysis performed on the hemagglutinin gene segment showed that bear and gull strains shared 99.998% genetic identity, making the bird strain the closest related strain. We detected the PB2 E627K mutation in minute quantities in the gull, whereas it predominated in the bear, which suggests that this mammalian adaptation marker was selected during the bear infection. Our results provide the first molecular and histopathological characterization of an H5N1 virus infection in this bear species.IMPORTANCEAvian influenza viruses are able to cross the species barrier between birds and mammals because of their high genetic diversity and mutation rate. Using formalin-fixed paraffin-embedded tissues, we were able to investigate a Tibetan black bear's infection by a high pathogenicity H5N1 avian influenza virus at the molecular, phylogenetic, and histological levels. Our results highlight the importance of virological surveillance programs in mammals and the importance of raising awareness among veterinarians and zookeepers of the clinical presentations associated with H5Nx virus infection in mammals. Article in Journal/Newspaper Black-headed Gull Chroicocephalus ridibundus Directory of Open Access Journals: DOAJ Articles Microbiology Spectrum 12 3 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
influenza zoonotic infections epidemiology Microbiology QR1-502 |
spellingShingle |
influenza zoonotic infections epidemiology Microbiology QR1-502 Pierre Bessière Nicolas Gaide Guillaume Croville Manuela Crispo Maxime Fusade-Boyer Yanad Abou Monsef Malorie Dirat Marielle Beltrame Philippine Dendauw Karin Lemberger Jean-Luc Guérin Guillaume Le Loc'h High pathogenicity avian influenza A (H5N1) clade 2.3.4.4b virus infection in a captive Tibetan black bear (Ursus thibetanus): investigations based on paraffin-embedded tissues, France, 2022 |
topic_facet |
influenza zoonotic infections epidemiology Microbiology QR1-502 |
description |
ABSTRACTHigh pathogenicity avian influenza viruses (HPAIVs) H5Nx of clade 2.3.4.4b have been circulating increasingly in both wild and domestic birds in recent years. In turn, this has led to an increase in the number of spillover events affecting mammals. In November 2022, an HPAIV H5N1 caused an outbreak in a zoological park in the south of France, resulting in the death of a Tibetan black bear (Ursus thibetanus) and several captive and wild bird species. We detected the virus in various tissues of the bear and a wild black-headed gull (Chroicocephalus ridibundus) found dead in its enclosure using histopathology, two different in situ detection techniques, and next-generation sequencing, all performed on formalin-fixed paraffin-embedded tissues. Phylogenetic analysis performed on the hemagglutinin gene segment showed that bear and gull strains shared 99.998% genetic identity, making the bird strain the closest related strain. We detected the PB2 E627K mutation in minute quantities in the gull, whereas it predominated in the bear, which suggests that this mammalian adaptation marker was selected during the bear infection. Our results provide the first molecular and histopathological characterization of an H5N1 virus infection in this bear species.IMPORTANCEAvian influenza viruses are able to cross the species barrier between birds and mammals because of their high genetic diversity and mutation rate. Using formalin-fixed paraffin-embedded tissues, we were able to investigate a Tibetan black bear's infection by a high pathogenicity H5N1 avian influenza virus at the molecular, phylogenetic, and histological levels. Our results highlight the importance of virological surveillance programs in mammals and the importance of raising awareness among veterinarians and zookeepers of the clinical presentations associated with H5Nx virus infection in mammals. |
format |
Article in Journal/Newspaper |
author |
Pierre Bessière Nicolas Gaide Guillaume Croville Manuela Crispo Maxime Fusade-Boyer Yanad Abou Monsef Malorie Dirat Marielle Beltrame Philippine Dendauw Karin Lemberger Jean-Luc Guérin Guillaume Le Loc'h |
author_facet |
Pierre Bessière Nicolas Gaide Guillaume Croville Manuela Crispo Maxime Fusade-Boyer Yanad Abou Monsef Malorie Dirat Marielle Beltrame Philippine Dendauw Karin Lemberger Jean-Luc Guérin Guillaume Le Loc'h |
author_sort |
Pierre Bessière |
title |
High pathogenicity avian influenza A (H5N1) clade 2.3.4.4b virus infection in a captive Tibetan black bear (Ursus thibetanus): investigations based on paraffin-embedded tissues, France, 2022 |
title_short |
High pathogenicity avian influenza A (H5N1) clade 2.3.4.4b virus infection in a captive Tibetan black bear (Ursus thibetanus): investigations based on paraffin-embedded tissues, France, 2022 |
title_full |
High pathogenicity avian influenza A (H5N1) clade 2.3.4.4b virus infection in a captive Tibetan black bear (Ursus thibetanus): investigations based on paraffin-embedded tissues, France, 2022 |
title_fullStr |
High pathogenicity avian influenza A (H5N1) clade 2.3.4.4b virus infection in a captive Tibetan black bear (Ursus thibetanus): investigations based on paraffin-embedded tissues, France, 2022 |
title_full_unstemmed |
High pathogenicity avian influenza A (H5N1) clade 2.3.4.4b virus infection in a captive Tibetan black bear (Ursus thibetanus): investigations based on paraffin-embedded tissues, France, 2022 |
title_sort |
high pathogenicity avian influenza a (h5n1) clade 2.3.4.4b virus infection in a captive tibetan black bear (ursus thibetanus): investigations based on paraffin-embedded tissues, france, 2022 |
publisher |
American Society for Microbiology |
publishDate |
2024 |
url |
https://doi.org/10.1128/spectrum.03736-23 https://doaj.org/article/cb60127ebead4a26aa76bb13de5274a1 |
genre |
Black-headed Gull Chroicocephalus ridibundus |
genre_facet |
Black-headed Gull Chroicocephalus ridibundus |
op_source |
Microbiology Spectrum, Vol 12, Iss 3 (2024) |
op_relation |
https://journals.asm.org/doi/10.1128/spectrum.03736-23 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.03736-23 2165-0497 https://doaj.org/article/cb60127ebead4a26aa76bb13de5274a1 |
op_doi |
https://doi.org/10.1128/spectrum.03736-23 |
container_title |
Microbiology Spectrum |
container_volume |
12 |
container_issue |
3 |
_version_ |
1810486000473866240 |