Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils
Rising temperatures in the Arctic have led to the thawing of tundra soils, which is rapidly changing terrain, hydrology, and plant and microbial communities, causing hotspots of biogeochemical activity across the landscape. Despite this, little is known about how nutrient-rich low molecular weight d...
Published in: | Soil Systems |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3390/soilsystems5010010 https://doaj.org/article/cb40e367619f41efa6a22d69fc8e8260 |
id |
ftdoajarticles:oai:doaj.org/article:cb40e367619f41efa6a22d69fc8e8260 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:cb40e367619f41efa6a22d69fc8e8260 2024-01-07T09:41:14+01:00 Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils Mallory P. Ladd David T. Reeves Suresh Poudel Colleen M. Iversen Stan D. Wullschleger Robert L. Hettich 2021-02-01T00:00:00Z https://doi.org/10.3390/soilsystems5010010 https://doaj.org/article/cb40e367619f41efa6a22d69fc8e8260 EN eng MDPI AG https://www.mdpi.com/2571-8789/5/1/10 https://doaj.org/toc/2571-8789 doi:10.3390/soilsystems5010010 2571-8789 https://doaj.org/article/cb40e367619f41efa6a22d69fc8e8260 Soil Systems, Vol 5, Iss 1, p 10 (2021) untargeted metabolomics nano-liquid chromatography/mass spectrometry Arctic soil dissolved organic matter Physical geography GB3-5030 Chemistry QD1-999 article 2021 ftdoajarticles https://doi.org/10.3390/soilsystems5010010 2023-12-10T01:46:28Z Rising temperatures in the Arctic have led to the thawing of tundra soils, which is rapidly changing terrain, hydrology, and plant and microbial communities, causing hotspots of biogeochemical activity across the landscape. Despite this, little is known about how nutrient-rich low molecular weight dissolved organic matter (LMW DOM) varies within and across tundra ecosystems. Using a high-resolution nano-liquid chromatography-mass spectrometry (LC/MS) approach, we characterized the composition and availability of LMW DOM from high-centered polygons (HCP) and low-centered polygons (LCP) with Eriophorum angustifolium or Carex aquatilis as the dominant vegetation. Over 3000 unique features (i.e., discrete mass/charge ions) were detected; 521 were identified as differentially abundant between polygonal types and 217 were putatively annotated using high mass accuracy MS data. While polygon type was a strong predictor of LMW DOM composition and availability, vegetation and soil depth were also important drivers. Extensive evidence was found for enhanced microbial processing at the LCP sites, which were dominated by Carex plant species. We detected significant differences between polygon types with varying aboveground landscape features or properties, and hotspots of biogeochemical activity, indicating LMW DOM, as quantified by untargeted exometabolomics, provides a window into the dynamic complex interactions between landscape topography, vegetation, and organic matter cycling in Arctic polygonal tundra soils. Article in Journal/Newspaper Arctic Carex aquatilis Eriophorum Tundra Directory of Open Access Journals: DOAJ Articles Arctic Soil Systems 5 1 10 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
untargeted metabolomics nano-liquid chromatography/mass spectrometry Arctic soil dissolved organic matter Physical geography GB3-5030 Chemistry QD1-999 |
spellingShingle |
untargeted metabolomics nano-liquid chromatography/mass spectrometry Arctic soil dissolved organic matter Physical geography GB3-5030 Chemistry QD1-999 Mallory P. Ladd David T. Reeves Suresh Poudel Colleen M. Iversen Stan D. Wullschleger Robert L. Hettich Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
topic_facet |
untargeted metabolomics nano-liquid chromatography/mass spectrometry Arctic soil dissolved organic matter Physical geography GB3-5030 Chemistry QD1-999 |
description |
Rising temperatures in the Arctic have led to the thawing of tundra soils, which is rapidly changing terrain, hydrology, and plant and microbial communities, causing hotspots of biogeochemical activity across the landscape. Despite this, little is known about how nutrient-rich low molecular weight dissolved organic matter (LMW DOM) varies within and across tundra ecosystems. Using a high-resolution nano-liquid chromatography-mass spectrometry (LC/MS) approach, we characterized the composition and availability of LMW DOM from high-centered polygons (HCP) and low-centered polygons (LCP) with Eriophorum angustifolium or Carex aquatilis as the dominant vegetation. Over 3000 unique features (i.e., discrete mass/charge ions) were detected; 521 were identified as differentially abundant between polygonal types and 217 were putatively annotated using high mass accuracy MS data. While polygon type was a strong predictor of LMW DOM composition and availability, vegetation and soil depth were also important drivers. Extensive evidence was found for enhanced microbial processing at the LCP sites, which were dominated by Carex plant species. We detected significant differences between polygon types with varying aboveground landscape features or properties, and hotspots of biogeochemical activity, indicating LMW DOM, as quantified by untargeted exometabolomics, provides a window into the dynamic complex interactions between landscape topography, vegetation, and organic matter cycling in Arctic polygonal tundra soils. |
format |
Article in Journal/Newspaper |
author |
Mallory P. Ladd David T. Reeves Suresh Poudel Colleen M. Iversen Stan D. Wullschleger Robert L. Hettich |
author_facet |
Mallory P. Ladd David T. Reeves Suresh Poudel Colleen M. Iversen Stan D. Wullschleger Robert L. Hettich |
author_sort |
Mallory P. Ladd |
title |
Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
title_short |
Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
title_full |
Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
title_fullStr |
Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
title_full_unstemmed |
Untargeted Exometabolomics Provides a Powerful Approach to Investigate Biogeochemical Hotspots with Vegetation and Polygon Type in Arctic Tundra Soils |
title_sort |
untargeted exometabolomics provides a powerful approach to investigate biogeochemical hotspots with vegetation and polygon type in arctic tundra soils |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/soilsystems5010010 https://doaj.org/article/cb40e367619f41efa6a22d69fc8e8260 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Carex aquatilis Eriophorum Tundra |
genre_facet |
Arctic Carex aquatilis Eriophorum Tundra |
op_source |
Soil Systems, Vol 5, Iss 1, p 10 (2021) |
op_relation |
https://www.mdpi.com/2571-8789/5/1/10 https://doaj.org/toc/2571-8789 doi:10.3390/soilsystems5010010 2571-8789 https://doaj.org/article/cb40e367619f41efa6a22d69fc8e8260 |
op_doi |
https://doi.org/10.3390/soilsystems5010010 |
container_title |
Soil Systems |
container_volume |
5 |
container_issue |
1 |
container_start_page |
10 |
_version_ |
1787422051595714560 |