Malaria vectors of Timor-Leste
Abstract Background The island of Timor lies at the south-eastern edge of Indonesia on the boundary of the Oriental and Australian faunal regions. The country of Timor-Leste, which occupies the eastern part of the island, is malarious but anopheline faunal surveys and malaria vector incrimination da...
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ftdoajarticles:oai:doaj.org/article:ca949316517b44baac70b9246a2e3f98 2023-05-15T15:15:56+02:00 Malaria vectors of Timor-Leste Frances Stephen P Edstein Michael D Cooper Robert D Beebe Nigel W 2010-02-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-40 https://doaj.org/article/ca949316517b44baac70b9246a2e3f98 EN eng BMC http://www.malariajournal.com/content/9/1/40 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-40 1475-2875 https://doaj.org/article/ca949316517b44baac70b9246a2e3f98 Malaria Journal, Vol 9, Iss 1, p 40 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-40 2022-12-31T00:28:41Z Abstract Background The island of Timor lies at the south-eastern edge of Indonesia on the boundary of the Oriental and Australian faunal regions. The country of Timor-Leste, which occupies the eastern part of the island, is malarious but anopheline faunal surveys and malaria vector incrimination date back to the 1960 s. Over the last decade the malaria vectors of south-east Asia and the south-west Pacific have been intensely studied using molecular techniques that can confirm identification within complexes of isomorphic species. The aim of this study is to accurately identify the Anopheles fauna of Timor-Leste using these techniques. Methods The survey was carried out over the period February to June 2001. Standard entomological techniques - human landing collections, larval collections and CO2 baited light traps - were used to collect anophelines from the main geographical regions: coastal plains, inland plains, and highlands. Specimens were processed for identification by morphology and genotyped for the ribosomal DNA ITS2 by restriction analysis and/or DNA sequencing. Phylogenetic relationship of Anopheles sundaicus and Anopheles subpictus individuals was also assessed using DNA sequences from the ITS2 and mitochondrial cytochrome-b. All specimens, other than those from larval surveys, were processed to detect the presence of the Plasmodium parasite circumsporozoite protein by ELISA for vector incrimination. Results Of 2,030 specimens collected, seven species were identified by morphology: Anopheles barbirostris, Anopheles aconitus, Anopheles annularis, Anopheles maculatus, Anopheles peditaeniatus, An. sundaicus and Anopheles vagus . These were confirmed by molecular analysis with the addition of Anopheles flavirostris and an unidentified species designated here as An. vagus genotype B. This latter species was morphologically similar to An. vagus and An. subpictus and is likely to be the An. subpictus described by other workers for Timor. However, genetically this species showed strong affinities to the An. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Pacific Malaria Journal 9 1 |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Frances Stephen P Edstein Michael D Cooper Robert D Beebe Nigel W Malaria vectors of Timor-Leste |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The island of Timor lies at the south-eastern edge of Indonesia on the boundary of the Oriental and Australian faunal regions. The country of Timor-Leste, which occupies the eastern part of the island, is malarious but anopheline faunal surveys and malaria vector incrimination date back to the 1960 s. Over the last decade the malaria vectors of south-east Asia and the south-west Pacific have been intensely studied using molecular techniques that can confirm identification within complexes of isomorphic species. The aim of this study is to accurately identify the Anopheles fauna of Timor-Leste using these techniques. Methods The survey was carried out over the period February to June 2001. Standard entomological techniques - human landing collections, larval collections and CO2 baited light traps - were used to collect anophelines from the main geographical regions: coastal plains, inland plains, and highlands. Specimens were processed for identification by morphology and genotyped for the ribosomal DNA ITS2 by restriction analysis and/or DNA sequencing. Phylogenetic relationship of Anopheles sundaicus and Anopheles subpictus individuals was also assessed using DNA sequences from the ITS2 and mitochondrial cytochrome-b. All specimens, other than those from larval surveys, were processed to detect the presence of the Plasmodium parasite circumsporozoite protein by ELISA for vector incrimination. Results Of 2,030 specimens collected, seven species were identified by morphology: Anopheles barbirostris, Anopheles aconitus, Anopheles annularis, Anopheles maculatus, Anopheles peditaeniatus, An. sundaicus and Anopheles vagus . These were confirmed by molecular analysis with the addition of Anopheles flavirostris and an unidentified species designated here as An. vagus genotype B. This latter species was morphologically similar to An. vagus and An. subpictus and is likely to be the An. subpictus described by other workers for Timor. However, genetically this species showed strong affinities to the An. ... |
format |
Article in Journal/Newspaper |
author |
Frances Stephen P Edstein Michael D Cooper Robert D Beebe Nigel W |
author_facet |
Frances Stephen P Edstein Michael D Cooper Robert D Beebe Nigel W |
author_sort |
Frances Stephen P |
title |
Malaria vectors of Timor-Leste |
title_short |
Malaria vectors of Timor-Leste |
title_full |
Malaria vectors of Timor-Leste |
title_fullStr |
Malaria vectors of Timor-Leste |
title_full_unstemmed |
Malaria vectors of Timor-Leste |
title_sort |
malaria vectors of timor-leste |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-40 https://doaj.org/article/ca949316517b44baac70b9246a2e3f98 |
geographic |
Arctic Pacific |
geographic_facet |
Arctic Pacific |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 40 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/40 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-40 1475-2875 https://doaj.org/article/ca949316517b44baac70b9246a2e3f98 |
op_doi |
https://doi.org/10.1186/1475-2875-9-40 |
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Malaria Journal |
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9 |
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1 |
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1766346265904283648 |