In vitro production of peroxynitrite by haemocytes from marine bivalves: C-ELISA determination of 3-nitrotyrosine level in plasma proteins from Mytilus galloprovincialis and Crassostrea gigas

• Published in: BMC Immunology
• Main Authors: Romestand Bernard, Torreilles Jean
• Format: Article in Journal/Newspaper
• Language: English
• Published: BMC 2001
• Subjects: Immunologic diseases. Allergy; RC581-607; Crassostrea gigas
• Online Access: https://doi.org/10.1186/1471-2172-2-1; https://doaj.org/article/ca83d81652ff45ac8994458f3580e458

Abstract Background Peroxynitrite is increasingly proposed as a contributor to defence system in marine bivalve. It can be formed by combinaison of superoxide and nitric oxide, and can react with tyrosine residues of proteins giving rise to 3-nitrotyrosine. Results The present article describes a competitive ELISA for the measurement of 3-nitrotyrosine contents of plasma proteins from marine bivalves by means of a monoclonal anti 3-nitrotyrosine antibody mouse IgG. Conclusions This assay is sensitive enough to determine the amounts of 3-nitrotyrosine in plasma proteins from one animal only. Using the C-ELISA, we have shown that the phagocytosis of zymosan particles increased the 3-nitrotyrosine levels of plasma proteins from mussel M. galloprovincialis and oyster C. gigas 5.8 and 7.5 times respectively.