Plasmodium falciparum resistance to anti-malarial drugs in Papua New Guinea: evaluation of a community-based approach for the molecular monitoring of resistance
Abstract Background Molecular monitoring of parasite resistance has become an important complementary tool in establishing rational anti-malarial drug policies. Community surveys provide a representative sample of the parasite population and can be carried out more rapidly than accrual of samples fr...
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ftdoajarticles:oai:doaj.org/article:ca603f3928184b648e38853b5f13c2bb 2023-05-15T15:14:31+02:00 Plasmodium falciparum resistance to anti-malarial drugs in Papua New Guinea: evaluation of a community-based approach for the molecular monitoring of resistance Reeder John C Baisor Moses Oa Olive Sie Albert Müller Ivo Hastings Ian M Smith Thomas A Marfurt Jutta Beck Hans-Peter Genton Blaise 2010-01-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-8 https://doaj.org/article/ca603f3928184b648e38853b5f13c2bb EN eng BMC http://www.malariajournal.com/content/9/1/8 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-8 1475-2875 https://doaj.org/article/ca603f3928184b648e38853b5f13c2bb Malaria Journal, Vol 9, Iss 1, p 8 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-8 2022-12-31T07:25:56Z Abstract Background Molecular monitoring of parasite resistance has become an important complementary tool in establishing rational anti-malarial drug policies. Community surveys provide a representative sample of the parasite population and can be carried out more rapidly than accrual of samples from clinical cases, but it is not known whether the frequencies of genetic resistance markers in clinical cases differ from those in the overall population, or whether such community surveys can provide good predictions of treatment failure rates. Methods Between 2003 and 2005, in vivo drug efficacy of amodiaquine or chloroquine plus sulphadoxine-pyrimethamine was determined at three sites in Papua New Guinea. The genetic drug resistance profile (i.e., 33 single nucleotide polymorphisms in Plasmodium falciparum crt , mdr1 , dhfr , dhps , and ATPase6 ) was concurrently assessed in 639 community samples collected in the catchment areas of the respective health facilities by using a DNA microarray-based method. Mutant allele and haplotype frequencies were determined and their relationship with treatment failure rates at each site in each year was investigated. Results PCR-corrected in vivo treatment failure rates were between 12% and 28% and varied by site and year with variable longitudinal trends. In the community samples, the frequencies of mutations in pfcrt and pfmdr1 were high and did not show significant changes over time. Mutant allele frequencies in pfdhfr were moderate and those in pfdhps were low. No mutations were detected in pfATPase6 . There was much more variation between sites than temporal, within-site, variation in allele and haplotype frequencies. This variation did not correlate well with treatment failure rates. Allele and haplotype frequencies were very similar in clinical and community samples from the same site. Conclusions The relationship between parasite genetics and in vivo treatment failure rate is not straightforward. The frequencies of genetic anti-malarial resistance markers appear to be ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 8 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Reeder John C Baisor Moses Oa Olive Sie Albert Müller Ivo Hastings Ian M Smith Thomas A Marfurt Jutta Beck Hans-Peter Genton Blaise Plasmodium falciparum resistance to anti-malarial drugs in Papua New Guinea: evaluation of a community-based approach for the molecular monitoring of resistance |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Molecular monitoring of parasite resistance has become an important complementary tool in establishing rational anti-malarial drug policies. Community surveys provide a representative sample of the parasite population and can be carried out more rapidly than accrual of samples from clinical cases, but it is not known whether the frequencies of genetic resistance markers in clinical cases differ from those in the overall population, or whether such community surveys can provide good predictions of treatment failure rates. Methods Between 2003 and 2005, in vivo drug efficacy of amodiaquine or chloroquine plus sulphadoxine-pyrimethamine was determined at three sites in Papua New Guinea. The genetic drug resistance profile (i.e., 33 single nucleotide polymorphisms in Plasmodium falciparum crt , mdr1 , dhfr , dhps , and ATPase6 ) was concurrently assessed in 639 community samples collected in the catchment areas of the respective health facilities by using a DNA microarray-based method. Mutant allele and haplotype frequencies were determined and their relationship with treatment failure rates at each site in each year was investigated. Results PCR-corrected in vivo treatment failure rates were between 12% and 28% and varied by site and year with variable longitudinal trends. In the community samples, the frequencies of mutations in pfcrt and pfmdr1 were high and did not show significant changes over time. Mutant allele frequencies in pfdhfr were moderate and those in pfdhps were low. No mutations were detected in pfATPase6 . There was much more variation between sites than temporal, within-site, variation in allele and haplotype frequencies. This variation did not correlate well with treatment failure rates. Allele and haplotype frequencies were very similar in clinical and community samples from the same site. Conclusions The relationship between parasite genetics and in vivo treatment failure rate is not straightforward. The frequencies of genetic anti-malarial resistance markers appear to be ... |
format |
Article in Journal/Newspaper |
author |
Reeder John C Baisor Moses Oa Olive Sie Albert Müller Ivo Hastings Ian M Smith Thomas A Marfurt Jutta Beck Hans-Peter Genton Blaise |
author_facet |
Reeder John C Baisor Moses Oa Olive Sie Albert Müller Ivo Hastings Ian M Smith Thomas A Marfurt Jutta Beck Hans-Peter Genton Blaise |
author_sort |
Reeder John C |
title |
Plasmodium falciparum resistance to anti-malarial drugs in Papua New Guinea: evaluation of a community-based approach for the molecular monitoring of resistance |
title_short |
Plasmodium falciparum resistance to anti-malarial drugs in Papua New Guinea: evaluation of a community-based approach for the molecular monitoring of resistance |
title_full |
Plasmodium falciparum resistance to anti-malarial drugs in Papua New Guinea: evaluation of a community-based approach for the molecular monitoring of resistance |
title_fullStr |
Plasmodium falciparum resistance to anti-malarial drugs in Papua New Guinea: evaluation of a community-based approach for the molecular monitoring of resistance |
title_full_unstemmed |
Plasmodium falciparum resistance to anti-malarial drugs in Papua New Guinea: evaluation of a community-based approach for the molecular monitoring of resistance |
title_sort |
plasmodium falciparum resistance to anti-malarial drugs in papua new guinea: evaluation of a community-based approach for the molecular monitoring of resistance |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-8 https://doaj.org/article/ca603f3928184b648e38853b5f13c2bb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 8 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/8 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-8 1475-2875 https://doaj.org/article/ca603f3928184b648e38853b5f13c2bb |
op_doi |
https://doi.org/10.1186/1475-2875-9-8 |
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Malaria Journal |
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9 |
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1 |
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8 |
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1766344963155558400 |