Evolution of subpatent parasitaemia in Trypanosoma cruzi chronically infected mice with the help of a cyclophosphamide amplification transfer assay Evolução das parasitemias subpatentes na infecção crônica experimental pelo Trypanosoma cruzi através do ensaio de subinoculação modificado pelo tratamento com ciclofosfamida
We have evaluated the sensitivity of the classical blood subinoculation method, modified through cyclophosphamide treatment of transferred mice, for the detection of occult parasitaemias in Trypanosoma cruzi chronically infected mice. Besides its simplicity, the method was shown to be highly sensiti...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
1991
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ftdoajarticles:oai:doaj.org/article:ca4c45816dce47c296c701a3942ff309 2024-09-09T19:28:26+00:00 Evolution of subpatent parasitaemia in Trypanosoma cruzi chronically infected mice with the help of a cyclophosphamide amplification transfer assay Evolução das parasitemias subpatentes na infecção crônica experimental pelo Trypanosoma cruzi através do ensaio de subinoculação modificado pelo tratamento com ciclofosfamida José M. Alvarez Ayumi Oshima Veronica Mozer Liliane Guimarães Hércules Menezes 1991-12-01T00:00:00Z https://doi.org/10.1590/S0036-46651991000600013 https://doaj.org/article/ca4c45816dce47c296c701a3942ff309 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651991000600013 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651991000600013 0036-4665 1678-9946 https://doaj.org/article/ca4c45816dce47c296c701a3942ff309 Revista do Instituto de Medicina Tropical de São Paulo, Vol 33, Iss 6, Pp 509-514 (1991) Trypanosoma cruzi Infection in mice Parasitaemia Cyclophosphamide amplification transfer assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1991 ftdoajarticles https://doi.org/10.1590/S0036-46651991000600013 2024-08-05T17:49:30Z We have evaluated the sensitivity of the classical blood subinoculation method, modified through cyclophosphamide treatment of transferred mice, for the detection of occult parasitaemias in Trypanosoma cruzi chronically infected mice. Besides its simplicity, the method was shown to be highly sensitive for both the "chronic" phase parasites (99% of chronic cases were shown to harbour occult parasitaemias) and for the acute phase parasites (T. cruzi could be detected in 53.8% of animals transferred with one Y strain parasite and in 20% of animals transferred with one CL strain parasite). Using acute phase bloodforms, the assay proved to be more sensitive than conventional subinoculation when dealing with the CL, but not the Y strain of the parasite. With the help of this parasite detection tool, we have studied during a one year period, the evolution of subpatent parasitaemias in a group of mice which survived through chemotherapy from lethal acute phase of T. cruzi infection. Cyclophosphamide transfer assay revealed occult parasitaemias in 100% of the chronic animals, nevertheless, continuous and discontinuous patterns of positivity were observed. Avaliamos o potencial do ensaio clássico de subinoculação, modificado pelo tratamento com ciclofosfamida dos animais receptores, na detecção de parasitemias ocultas em camundongos com in-fecção crônica pelo Trypanosoma cruzi. O ensaio, além de simples, mostrou ter uma alta sensibilidade; assim, utilizando-se parasitas da fase aguda, o tratamento com ciclofosfamida revelou parasitemias em 53,8% dos animais infectados com um tripanosoma da cepa y, e em 20% dos animais infectados com um tripanosoma da cepa CL. O tratamento com ciclofosfamida aumentou a sensibilidade do ensaio de subinoculação nas infecções pela cepa CL, e resultou em igual sensibilidade quando utilizada a cepa Y. Nos camundongos de fase crônica, obtidos a partir de diversos esquemas de imunoprofilaxia (BCG, soro de camundongo imune) ou quimioterapia, o ensaio revelou parasitemias ocultas em 99% dos ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Aguda ENVELOPE(-26.750,-26.750,-76.150,-76.150) Alta Arctic The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) Revista do Instituto de Medicina Tropical de São Paulo 33 6 509 514 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Trypanosoma cruzi Infection in mice Parasitaemia Cyclophosphamide amplification transfer assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Trypanosoma cruzi Infection in mice Parasitaemia Cyclophosphamide amplification transfer assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 José M. Alvarez Ayumi Oshima Veronica Mozer Liliane Guimarães Hércules Menezes Evolution of subpatent parasitaemia in Trypanosoma cruzi chronically infected mice with the help of a cyclophosphamide amplification transfer assay Evolução das parasitemias subpatentes na infecção crônica experimental pelo Trypanosoma cruzi através do ensaio de subinoculação modificado pelo tratamento com ciclofosfamida |
topic_facet |
Trypanosoma cruzi Infection in mice Parasitaemia Cyclophosphamide amplification transfer assay Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
We have evaluated the sensitivity of the classical blood subinoculation method, modified through cyclophosphamide treatment of transferred mice, for the detection of occult parasitaemias in Trypanosoma cruzi chronically infected mice. Besides its simplicity, the method was shown to be highly sensitive for both the "chronic" phase parasites (99% of chronic cases were shown to harbour occult parasitaemias) and for the acute phase parasites (T. cruzi could be detected in 53.8% of animals transferred with one Y strain parasite and in 20% of animals transferred with one CL strain parasite). Using acute phase bloodforms, the assay proved to be more sensitive than conventional subinoculation when dealing with the CL, but not the Y strain of the parasite. With the help of this parasite detection tool, we have studied during a one year period, the evolution of subpatent parasitaemias in a group of mice which survived through chemotherapy from lethal acute phase of T. cruzi infection. Cyclophosphamide transfer assay revealed occult parasitaemias in 100% of the chronic animals, nevertheless, continuous and discontinuous patterns of positivity were observed. Avaliamos o potencial do ensaio clássico de subinoculação, modificado pelo tratamento com ciclofosfamida dos animais receptores, na detecção de parasitemias ocultas em camundongos com in-fecção crônica pelo Trypanosoma cruzi. O ensaio, além de simples, mostrou ter uma alta sensibilidade; assim, utilizando-se parasitas da fase aguda, o tratamento com ciclofosfamida revelou parasitemias em 53,8% dos animais infectados com um tripanosoma da cepa y, e em 20% dos animais infectados com um tripanosoma da cepa CL. O tratamento com ciclofosfamida aumentou a sensibilidade do ensaio de subinoculação nas infecções pela cepa CL, e resultou em igual sensibilidade quando utilizada a cepa Y. Nos camundongos de fase crônica, obtidos a partir de diversos esquemas de imunoprofilaxia (BCG, soro de camundongo imune) ou quimioterapia, o ensaio revelou parasitemias ocultas em 99% dos ... |
format |
Article in Journal/Newspaper |
author |
José M. Alvarez Ayumi Oshima Veronica Mozer Liliane Guimarães Hércules Menezes |
author_facet |
José M. Alvarez Ayumi Oshima Veronica Mozer Liliane Guimarães Hércules Menezes |
author_sort |
José M. Alvarez |
title |
Evolution of subpatent parasitaemia in Trypanosoma cruzi chronically infected mice with the help of a cyclophosphamide amplification transfer assay Evolução das parasitemias subpatentes na infecção crônica experimental pelo Trypanosoma cruzi através do ensaio de subinoculação modificado pelo tratamento com ciclofosfamida |
title_short |
Evolution of subpatent parasitaemia in Trypanosoma cruzi chronically infected mice with the help of a cyclophosphamide amplification transfer assay Evolução das parasitemias subpatentes na infecção crônica experimental pelo Trypanosoma cruzi através do ensaio de subinoculação modificado pelo tratamento com ciclofosfamida |
title_full |
Evolution of subpatent parasitaemia in Trypanosoma cruzi chronically infected mice with the help of a cyclophosphamide amplification transfer assay Evolução das parasitemias subpatentes na infecção crônica experimental pelo Trypanosoma cruzi através do ensaio de subinoculação modificado pelo tratamento com ciclofosfamida |
title_fullStr |
Evolution of subpatent parasitaemia in Trypanosoma cruzi chronically infected mice with the help of a cyclophosphamide amplification transfer assay Evolução das parasitemias subpatentes na infecção crônica experimental pelo Trypanosoma cruzi através do ensaio de subinoculação modificado pelo tratamento com ciclofosfamida |
title_full_unstemmed |
Evolution of subpatent parasitaemia in Trypanosoma cruzi chronically infected mice with the help of a cyclophosphamide amplification transfer assay Evolução das parasitemias subpatentes na infecção crônica experimental pelo Trypanosoma cruzi através do ensaio de subinoculação modificado pelo tratamento com ciclofosfamida |
title_sort |
evolution of subpatent parasitaemia in trypanosoma cruzi chronically infected mice with the help of a cyclophosphamide amplification transfer assay evolução das parasitemias subpatentes na infecção crônica experimental pelo trypanosoma cruzi através do ensaio de subinoculação modificado pelo tratamento com ciclofosfamida |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1991 |
url |
https://doi.org/10.1590/S0036-46651991000600013 https://doaj.org/article/ca4c45816dce47c296c701a3942ff309 |
long_lat |
ENVELOPE(-26.750,-26.750,-76.150,-76.150) ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
Aguda Alta Arctic The ''Y'' |
geographic_facet |
Aguda Alta Arctic The ''Y'' |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 33, Iss 6, Pp 509-514 (1991) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651991000600013 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651991000600013 0036-4665 1678-9946 https://doaj.org/article/ca4c45816dce47c296c701a3942ff309 |
op_doi |
https://doi.org/10.1590/S0036-46651991000600013 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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33 |
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