Allovahlkampfia spelaea Causing Keratitis in Humans.
BACKGROUND:Free-living amoebae are present worldwide. They can survive in different environment causing human diseases in some instances. Acanthamoeba sp. is known for causing sight-threatening keratitis in humans. Free-living amoeba keratitis is more common in developing countries. Amoebae of famil...
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ftdoajarticles:oai:doaj.org/article:ca2f53541aba4053907f9ac0fa0374d1 2023-05-15T15:07:55+02:00 Allovahlkampfia spelaea Causing Keratitis in Humans. Mohammed Essa Marghany Tolba Enas Abdelhameed Mahmoud Huseein Haiam Mohamed Mahmoud Farrag Hanan El Deek Mohamed Seiki Kobayashi Jun Suzuki Tarek Ahmed Mohamed Ali Sumio Sugano 2016-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004841 https://doaj.org/article/ca2f53541aba4053907f9ac0fa0374d1 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4945048?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004841 https://doaj.org/article/ca2f53541aba4053907f9ac0fa0374d1 PLoS Neglected Tropical Diseases, Vol 10, Iss 7, p e0004841 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004841 2022-12-30T21:57:31Z BACKGROUND:Free-living amoebae are present worldwide. They can survive in different environment causing human diseases in some instances. Acanthamoeba sp. is known for causing sight-threatening keratitis in humans. Free-living amoeba keratitis is more common in developing countries. Amoebae of family Vahlkampfiidae are rarely reported to cause such affections. A new genus, Allovahlkampfia spelaea was recently identified from caves with no data about pathogenicity in humans. We tried to identify the causative free-living amoeba in a case of keratitis in an Egyptian patient using morphological and molecular techniques. METHODS:Pathogenic amoebae were culture using monoxenic culture system. Identification through morphological features and 18S ribosomal RNA subunit DNA amplification and sequencing was done. Pathogenicity to laboratory rabbits and ability to produce keratitis were assessed experimentally. RESULTS:Allovahlkampfia spelaea was identified as a cause of human keratitis. Whole sequence of 18S ribosomal subunit DNA was sequenced and assembled. The Egyptian strain was closely related to SK1 strain isolated in Slovenia. The ability to induce keratitis was confirmed using animal model. CONCLUSIONS:This the first time to report Allovahlkampfia spelaea as a human pathogen. Combining both molecular and morphological identification is critical to correctly diagnose amoebae causing keratitis in humans. Use of different pairs of primers and sequencing amplified DNA is needed to prevent misdiagnosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 7 e0004841 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Mohammed Essa Marghany Tolba Enas Abdelhameed Mahmoud Huseein Haiam Mohamed Mahmoud Farrag Hanan El Deek Mohamed Seiki Kobayashi Jun Suzuki Tarek Ahmed Mohamed Ali Sumio Sugano Allovahlkampfia spelaea Causing Keratitis in Humans. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Free-living amoebae are present worldwide. They can survive in different environment causing human diseases in some instances. Acanthamoeba sp. is known for causing sight-threatening keratitis in humans. Free-living amoeba keratitis is more common in developing countries. Amoebae of family Vahlkampfiidae are rarely reported to cause such affections. A new genus, Allovahlkampfia spelaea was recently identified from caves with no data about pathogenicity in humans. We tried to identify the causative free-living amoeba in a case of keratitis in an Egyptian patient using morphological and molecular techniques. METHODS:Pathogenic amoebae were culture using monoxenic culture system. Identification through morphological features and 18S ribosomal RNA subunit DNA amplification and sequencing was done. Pathogenicity to laboratory rabbits and ability to produce keratitis were assessed experimentally. RESULTS:Allovahlkampfia spelaea was identified as a cause of human keratitis. Whole sequence of 18S ribosomal subunit DNA was sequenced and assembled. The Egyptian strain was closely related to SK1 strain isolated in Slovenia. The ability to induce keratitis was confirmed using animal model. CONCLUSIONS:This the first time to report Allovahlkampfia spelaea as a human pathogen. Combining both molecular and morphological identification is critical to correctly diagnose amoebae causing keratitis in humans. Use of different pairs of primers and sequencing amplified DNA is needed to prevent misdiagnosis. |
format |
Article in Journal/Newspaper |
author |
Mohammed Essa Marghany Tolba Enas Abdelhameed Mahmoud Huseein Haiam Mohamed Mahmoud Farrag Hanan El Deek Mohamed Seiki Kobayashi Jun Suzuki Tarek Ahmed Mohamed Ali Sumio Sugano |
author_facet |
Mohammed Essa Marghany Tolba Enas Abdelhameed Mahmoud Huseein Haiam Mohamed Mahmoud Farrag Hanan El Deek Mohamed Seiki Kobayashi Jun Suzuki Tarek Ahmed Mohamed Ali Sumio Sugano |
author_sort |
Mohammed Essa Marghany Tolba |
title |
Allovahlkampfia spelaea Causing Keratitis in Humans. |
title_short |
Allovahlkampfia spelaea Causing Keratitis in Humans. |
title_full |
Allovahlkampfia spelaea Causing Keratitis in Humans. |
title_fullStr |
Allovahlkampfia spelaea Causing Keratitis in Humans. |
title_full_unstemmed |
Allovahlkampfia spelaea Causing Keratitis in Humans. |
title_sort |
allovahlkampfia spelaea causing keratitis in humans. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004841 https://doaj.org/article/ca2f53541aba4053907f9ac0fa0374d1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 7, p e0004841 (2016) |
op_relation |
http://europepmc.org/articles/PMC4945048?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004841 https://doaj.org/article/ca2f53541aba4053907f9ac0fa0374d1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004841 |
container_title |
PLOS Neglected Tropical Diseases |
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10 |
container_issue |
7 |
container_start_page |
e0004841 |
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