Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment
In this study, we isolated and characterized bacterial strains from ancient (Neogene) permafrost sediment that was permanently frozen for 3.5 million years. The sampling site was located at Mammoth Mountain in the Aldan river valley in Central Yakutia in Eastern Siberia. Analysis of phospolipid fatt...
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ftdoajarticles:oai:doaj.org/article:ca03a8ca487241b5ad9e85e76f5fe217 2023-10-09T21:55:10+02:00 Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment De-Chao Zhang Anatoli Brouchkov Gennady Griva Franz Schinner Rosa Margesin 2013-01-01T00:00:00Z https://doi.org/10.3390/biology2010085 https://doaj.org/article/ca03a8ca487241b5ad9e85e76f5fe217 EN eng MDPI AG http://www.mdpi.com/2079-7737/2/1/85 https://doaj.org/toc/2079-7737 doi:10.3390/biology2010085 2079-7737 https://doaj.org/article/ca03a8ca487241b5ad9e85e76f5fe217 Biology, Vol 2, Iss 1, Pp 85-106 (2013) permafrost ancient Neogene sediment Siberia microorganisms Arthrobacter Glaciimonas Subtercola Biology (General) QH301-705.5 article 2013 ftdoajarticles https://doi.org/10.3390/biology2010085 2023-09-10T00:49:57Z In this study, we isolated and characterized bacterial strains from ancient (Neogene) permafrost sediment that was permanently frozen for 3.5 million years. The sampling site was located at Mammoth Mountain in the Aldan river valley in Central Yakutia in Eastern Siberia. Analysis of phospolipid fatty acids (PLFA) demonstrated the dominance of bacteria over fungi; the analysis of fatty acids specific for Gram-positive and Gram-negative bacteria revealed an approximately twofold higher amount of Gram-negative bacteria compared to Gram-positive bacteria. Direct microbial counts after natural permafrost enrichment showed the presence of (4.7 ± 1.5) × 108 cells g−1 sediment dry mass. Viable heterotrophic bacteria were found at 0 °C, 10 °C and 25 °C, but not at 37 °C. Spore-forming bacteria were not detected. Numbers of viable fungi were low and were only detected at 0 °C and 10 °C. Selected culturable bacterial isolates were identified as representatives of Arthrobacter phenanthrenivorans, Subtercola frigoramans and Glaciimonas immobilis. Representatives of each of these species were characterized with regard to their growth temperature range, their ability to grow on different media, to produce enzymes, to grow in the presence of NaCl, antibiotics, and heavy metals, and to degrade hydrocarbons. All strains could grow at −5 °C; the upper temperature limit for growth in liquid culture was 25 °C or 30 °C. Sensitivity to rich media, antibiotics, heavy metals, and salt increased when temperature decreased (20 °C > 10 °C > 1 °C). In spite of the ligninolytic activity of some strains, no biodegradation activity was detected. Article in Journal/Newspaper permafrost Yakutia Siberia Directory of Open Access Journals: DOAJ Articles Aldan ENVELOPE(129.546,129.546,63.447,63.447) Biology 2 1 85 106 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
permafrost ancient Neogene sediment Siberia microorganisms Arthrobacter Glaciimonas Subtercola Biology (General) QH301-705.5 |
spellingShingle |
permafrost ancient Neogene sediment Siberia microorganisms Arthrobacter Glaciimonas Subtercola Biology (General) QH301-705.5 De-Chao Zhang Anatoli Brouchkov Gennady Griva Franz Schinner Rosa Margesin Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment |
topic_facet |
permafrost ancient Neogene sediment Siberia microorganisms Arthrobacter Glaciimonas Subtercola Biology (General) QH301-705.5 |
description |
In this study, we isolated and characterized bacterial strains from ancient (Neogene) permafrost sediment that was permanently frozen for 3.5 million years. The sampling site was located at Mammoth Mountain in the Aldan river valley in Central Yakutia in Eastern Siberia. Analysis of phospolipid fatty acids (PLFA) demonstrated the dominance of bacteria over fungi; the analysis of fatty acids specific for Gram-positive and Gram-negative bacteria revealed an approximately twofold higher amount of Gram-negative bacteria compared to Gram-positive bacteria. Direct microbial counts after natural permafrost enrichment showed the presence of (4.7 ± 1.5) × 108 cells g−1 sediment dry mass. Viable heterotrophic bacteria were found at 0 °C, 10 °C and 25 °C, but not at 37 °C. Spore-forming bacteria were not detected. Numbers of viable fungi were low and were only detected at 0 °C and 10 °C. Selected culturable bacterial isolates were identified as representatives of Arthrobacter phenanthrenivorans, Subtercola frigoramans and Glaciimonas immobilis. Representatives of each of these species were characterized with regard to their growth temperature range, their ability to grow on different media, to produce enzymes, to grow in the presence of NaCl, antibiotics, and heavy metals, and to degrade hydrocarbons. All strains could grow at −5 °C; the upper temperature limit for growth in liquid culture was 25 °C or 30 °C. Sensitivity to rich media, antibiotics, heavy metals, and salt increased when temperature decreased (20 °C > 10 °C > 1 °C). In spite of the ligninolytic activity of some strains, no biodegradation activity was detected. |
format |
Article in Journal/Newspaper |
author |
De-Chao Zhang Anatoli Brouchkov Gennady Griva Franz Schinner Rosa Margesin |
author_facet |
De-Chao Zhang Anatoli Brouchkov Gennady Griva Franz Schinner Rosa Margesin |
author_sort |
De-Chao Zhang |
title |
Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment |
title_short |
Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment |
title_full |
Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment |
title_fullStr |
Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment |
title_full_unstemmed |
Isolation and Characterization of Bacteria from Ancient Siberian Permafrost Sediment |
title_sort |
isolation and characterization of bacteria from ancient siberian permafrost sediment |
publisher |
MDPI AG |
publishDate |
2013 |
url |
https://doi.org/10.3390/biology2010085 https://doaj.org/article/ca03a8ca487241b5ad9e85e76f5fe217 |
long_lat |
ENVELOPE(129.546,129.546,63.447,63.447) |
geographic |
Aldan |
geographic_facet |
Aldan |
genre |
permafrost Yakutia Siberia |
genre_facet |
permafrost Yakutia Siberia |
op_source |
Biology, Vol 2, Iss 1, Pp 85-106 (2013) |
op_relation |
http://www.mdpi.com/2079-7737/2/1/85 https://doaj.org/toc/2079-7737 doi:10.3390/biology2010085 2079-7737 https://doaj.org/article/ca03a8ca487241b5ad9e85e76f5fe217 |
op_doi |
https://doi.org/10.3390/biology2010085 |
container_title |
Biology |
container_volume |
2 |
container_issue |
1 |
container_start_page |
85 |
op_container_end_page |
106 |
_version_ |
1779318994752241664 |