Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis Técnica da imunoperoxidase utilizando um soro hiperimune anti-Leishmania (L.) chagasi no diagnóstico da leishmaniose tegumentar americana confirmada por cultura
The present study reports the production of the rabbit anti-Leishmania (L.) chagasi hyperimmune serum, the standardization of the immunohistochemistry (IHC) technique and the evaluation of its employment in cutaneous leishmaniasis (CL) lesions diagnosed by Leishmania sp. culture isolation. Thirty fr...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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ftdoajarticles:oai:doaj.org/article:c9b68caf539d428083211186b11c8d5c 2024-09-09T19:28:11+00:00 Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis Técnica da imunoperoxidase utilizando um soro hiperimune anti-Leishmania (L.) chagasi no diagnóstico da leishmaniose tegumentar americana confirmada por cultura Leonardo P. Quintella Tullia Cuzzi Maria de F. Madeira Thais Okamoto Armando de O. Schubach 2009-04-01T00:00:00Z https://doi.org/10.1590/S0036-46652009000200005 https://doaj.org/article/c9b68caf539d428083211186b11c8d5c EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652009000200005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652009000200005 0036-4665 1678-9946 https://doaj.org/article/c9b68caf539d428083211186b11c8d5c Revista do Instituto de Medicina Tropical de São Paulo, Vol 51, Iss 2, Pp 83-86 (2009) Leishmaniasis Cutaneous Diagnosis Immunohistochemistry Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1590/S0036-46652009000200005 2024-08-05T17:49:30Z The present study reports the production of the rabbit anti-Leishmania (L.) chagasi hyperimmune serum, the standardization of the immunohistochemistry (IHC) technique and the evaluation of its employment in cutaneous leishmaniasis (CL) lesions diagnosed by Leishmania sp. culture isolation. Thirty fragments of active CL lesions were examined as well as 10 fragments of cutaneous mycosis lesions as control group. IHC proved more sensitive in detecting amastigotes than conventional hematoxylin-eosin (HE) stained slides: the former was positive in 24 (80%) biopsies whereas the latter, in 16 (53%) (p = 0.028). The reaction stained different fungus species causing cutaneous mycosis. Besides, positive reaction was noticed in mononuclear and endothelial cells. Nevertheless, this finding was present in the control group biopsies. It is concluded that IHC showed good sensitivity in detecting amastigotes. O presente estudo relata a produção do soro policlonal de coelho anti-Leishmania (L.) chagasi, a padronização da técnica de imunohistoquímica (IHQ) e sua aplicação em lesões de leishmaniose cutânea (LC) diagnosticadas por isolamento de Leishmania sp. em cultura. Foram examinados 30 fragmentos de lesões ativas de LC e 10 fragmentos de lesões de etiologia fúngica, utilizados como grupo controle. A IHQ mostrou-se mais sensível na detecção de amastigotas que a coloração em hematoxilina-eosina (HE), sendo positiva em 24 fragmentos de LC (80%) e ao passo que a HE foi positiva em 16 (53%) (p = 0,028). A IHQ também marcou diferentes espécies de fungos causadoras de micoses cutâneas. Adicionalmente, verificou-se positividade no citoplasma de células mononucleares e células endoteliais. Entretanto, esse achado esteve presente no grupo controle. Conclui-se que o método de IHQ apresentou boa sensibilidade na detecção de formas amastigotas. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Boa ENVELOPE(15.532,15.532,66.822,66.822) Revista do Instituto de Medicina Tropical de São Paulo 51 2 83 86 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Leishmaniasis Cutaneous Diagnosis Immunohistochemistry Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Leishmaniasis Cutaneous Diagnosis Immunohistochemistry Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Leonardo P. Quintella Tullia Cuzzi Maria de F. Madeira Thais Okamoto Armando de O. Schubach Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis Técnica da imunoperoxidase utilizando um soro hiperimune anti-Leishmania (L.) chagasi no diagnóstico da leishmaniose tegumentar americana confirmada por cultura |
topic_facet |
Leishmaniasis Cutaneous Diagnosis Immunohistochemistry Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The present study reports the production of the rabbit anti-Leishmania (L.) chagasi hyperimmune serum, the standardization of the immunohistochemistry (IHC) technique and the evaluation of its employment in cutaneous leishmaniasis (CL) lesions diagnosed by Leishmania sp. culture isolation. Thirty fragments of active CL lesions were examined as well as 10 fragments of cutaneous mycosis lesions as control group. IHC proved more sensitive in detecting amastigotes than conventional hematoxylin-eosin (HE) stained slides: the former was positive in 24 (80%) biopsies whereas the latter, in 16 (53%) (p = 0.028). The reaction stained different fungus species causing cutaneous mycosis. Besides, positive reaction was noticed in mononuclear and endothelial cells. Nevertheless, this finding was present in the control group biopsies. It is concluded that IHC showed good sensitivity in detecting amastigotes. O presente estudo relata a produção do soro policlonal de coelho anti-Leishmania (L.) chagasi, a padronização da técnica de imunohistoquímica (IHQ) e sua aplicação em lesões de leishmaniose cutânea (LC) diagnosticadas por isolamento de Leishmania sp. em cultura. Foram examinados 30 fragmentos de lesões ativas de LC e 10 fragmentos de lesões de etiologia fúngica, utilizados como grupo controle. A IHQ mostrou-se mais sensível na detecção de amastigotas que a coloração em hematoxilina-eosina (HE), sendo positiva em 24 fragmentos de LC (80%) e ao passo que a HE foi positiva em 16 (53%) (p = 0,028). A IHQ também marcou diferentes espécies de fungos causadoras de micoses cutâneas. Adicionalmente, verificou-se positividade no citoplasma de células mononucleares e células endoteliais. Entretanto, esse achado esteve presente no grupo controle. Conclui-se que o método de IHQ apresentou boa sensibilidade na detecção de formas amastigotas. |
format |
Article in Journal/Newspaper |
author |
Leonardo P. Quintella Tullia Cuzzi Maria de F. Madeira Thais Okamoto Armando de O. Schubach |
author_facet |
Leonardo P. Quintella Tullia Cuzzi Maria de F. Madeira Thais Okamoto Armando de O. Schubach |
author_sort |
Leonardo P. Quintella |
title |
Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis Técnica da imunoperoxidase utilizando um soro hiperimune anti-Leishmania (L.) chagasi no diagnóstico da leishmaniose tegumentar americana confirmada por cultura |
title_short |
Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis Técnica da imunoperoxidase utilizando um soro hiperimune anti-Leishmania (L.) chagasi no diagnóstico da leishmaniose tegumentar americana confirmada por cultura |
title_full |
Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis Técnica da imunoperoxidase utilizando um soro hiperimune anti-Leishmania (L.) chagasi no diagnóstico da leishmaniose tegumentar americana confirmada por cultura |
title_fullStr |
Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis Técnica da imunoperoxidase utilizando um soro hiperimune anti-Leishmania (L.) chagasi no diagnóstico da leishmaniose tegumentar americana confirmada por cultura |
title_full_unstemmed |
Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis Técnica da imunoperoxidase utilizando um soro hiperimune anti-Leishmania (L.) chagasi no diagnóstico da leishmaniose tegumentar americana confirmada por cultura |
title_sort |
immunoperoxidase technique using an anti-leishmania (l.) chagasi hyperimmune serum in the diagnosis of culture-confirmed american tegumentary leishmaniasis técnica da imunoperoxidase utilizando um soro hiperimune anti-leishmania (l.) chagasi no diagnóstico da leishmaniose tegumentar americana confirmada por cultura |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2009 |
url |
https://doi.org/10.1590/S0036-46652009000200005 https://doaj.org/article/c9b68caf539d428083211186b11c8d5c |
long_lat |
ENVELOPE(15.532,15.532,66.822,66.822) |
geographic |
Arctic Boa |
geographic_facet |
Arctic Boa |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 51, Iss 2, Pp 83-86 (2009) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652009000200005 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652009000200005 0036-4665 1678-9946 https://doaj.org/article/c9b68caf539d428083211186b11c8d5c |
op_doi |
https://doi.org/10.1590/S0036-46652009000200005 |
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51 |
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