Assessment of MALDI-TOF mass spectrometry for filariae detection in Aedes aegypti mosquitoes.
Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is an emerging tool for routine identification of bacteria, archaea and fungi. It has also been recently applied as an accurate approach for arthropod identification. Preliminary studies have shown that the M...
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ftdoajarticles:oai:doaj.org/article:c9a9852b276147948b8dc6131ea71233 2023-05-15T15:14:43+02:00 Assessment of MALDI-TOF mass spectrometry for filariae detection in Aedes aegypti mosquitoes. Djamel Tahir Lionel Almeras Marie Varloud Didier Raoult Bernard Davoust Philippe Parola 2017-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006093 https://doaj.org/article/c9a9852b276147948b8dc6131ea71233 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5754087?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006093 https://doaj.org/article/c9a9852b276147948b8dc6131ea71233 PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0006093 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006093 2022-12-31T15:58:38Z Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is an emerging tool for routine identification of bacteria, archaea and fungi. It has also been recently applied as an accurate approach for arthropod identification. Preliminary studies have shown that the MALDI-TOF MS was able to differentiate whether ticks and mosquitoes were infected or not with some bacteria and Plasmodium parasites, respectively. The aim of the present study was to test the efficiency of MALDI-TOF MS tool in distinguishing protein profiles between uninfected mosquitoes from specimens infected by filarioid helminths. Aedes aegypti mosquitoes were engorged on microfilaremic blood infected with Dirofilaria immitis, Brugia malayi or Brugia pahangi. Fifteen days post-infective blood feeding, a total of 534 mosquitoes were killed by freezing. To assess mass spectra (MS) profile changes following filariae infections, one compartment (legs, thorax, head or thorax and head) per mosquito was submitted for MALDI-TOF MS analysis; the remaining body parts were used to establish filariae infectious status by real-time qPCR. A database of reference MS, based on the mass profiles of at least two individual mosquitoes per compartment, was created. Subsequently, the remaining compartment spectra (N = 350) from Ae. aegypti infected or not infected by filariae were blind tested against the spectral database. In total, 37 discriminating peak masses ranging from 2062 to 14869 daltons were identified, of which 17, 11, 12 and 7 peak masses were for legs, thorax, thorax-head and head respectively. Two peak masses (4073 and 8847 Da) were specific to spectra from Ae. aegypti infected with filariae, regardless of nematode species or mosquito compartment. The thorax-head part provided better classification with a specificity of 94.1% and sensitivity of 86.6, 71.4 and 68.7% of D. immitis, B. malayi and B. pahangi respectively. This study presents the potential of MALDI-TOF MS as a reliable tool for differentiating non-infected ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 12 e0006093 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Djamel Tahir Lionel Almeras Marie Varloud Didier Raoult Bernard Davoust Philippe Parola Assessment of MALDI-TOF mass spectrometry for filariae detection in Aedes aegypti mosquitoes. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) is an emerging tool for routine identification of bacteria, archaea and fungi. It has also been recently applied as an accurate approach for arthropod identification. Preliminary studies have shown that the MALDI-TOF MS was able to differentiate whether ticks and mosquitoes were infected or not with some bacteria and Plasmodium parasites, respectively. The aim of the present study was to test the efficiency of MALDI-TOF MS tool in distinguishing protein profiles between uninfected mosquitoes from specimens infected by filarioid helminths. Aedes aegypti mosquitoes were engorged on microfilaremic blood infected with Dirofilaria immitis, Brugia malayi or Brugia pahangi. Fifteen days post-infective blood feeding, a total of 534 mosquitoes were killed by freezing. To assess mass spectra (MS) profile changes following filariae infections, one compartment (legs, thorax, head or thorax and head) per mosquito was submitted for MALDI-TOF MS analysis; the remaining body parts were used to establish filariae infectious status by real-time qPCR. A database of reference MS, based on the mass profiles of at least two individual mosquitoes per compartment, was created. Subsequently, the remaining compartment spectra (N = 350) from Ae. aegypti infected or not infected by filariae were blind tested against the spectral database. In total, 37 discriminating peak masses ranging from 2062 to 14869 daltons were identified, of which 17, 11, 12 and 7 peak masses were for legs, thorax, thorax-head and head respectively. Two peak masses (4073 and 8847 Da) were specific to spectra from Ae. aegypti infected with filariae, regardless of nematode species or mosquito compartment. The thorax-head part provided better classification with a specificity of 94.1% and sensitivity of 86.6, 71.4 and 68.7% of D. immitis, B. malayi and B. pahangi respectively. This study presents the potential of MALDI-TOF MS as a reliable tool for differentiating non-infected ... |
format |
Article in Journal/Newspaper |
author |
Djamel Tahir Lionel Almeras Marie Varloud Didier Raoult Bernard Davoust Philippe Parola |
author_facet |
Djamel Tahir Lionel Almeras Marie Varloud Didier Raoult Bernard Davoust Philippe Parola |
author_sort |
Djamel Tahir |
title |
Assessment of MALDI-TOF mass spectrometry for filariae detection in Aedes aegypti mosquitoes. |
title_short |
Assessment of MALDI-TOF mass spectrometry for filariae detection in Aedes aegypti mosquitoes. |
title_full |
Assessment of MALDI-TOF mass spectrometry for filariae detection in Aedes aegypti mosquitoes. |
title_fullStr |
Assessment of MALDI-TOF mass spectrometry for filariae detection in Aedes aegypti mosquitoes. |
title_full_unstemmed |
Assessment of MALDI-TOF mass spectrometry for filariae detection in Aedes aegypti mosquitoes. |
title_sort |
assessment of maldi-tof mass spectrometry for filariae detection in aedes aegypti mosquitoes. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006093 https://doaj.org/article/c9a9852b276147948b8dc6131ea71233 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0006093 (2017) |
op_relation |
http://europepmc.org/articles/PMC5754087?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006093 https://doaj.org/article/c9a9852b276147948b8dc6131ea71233 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006093 |
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PLOS Neglected Tropical Diseases |
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11 |
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12 |
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e0006093 |
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1766345143379558400 |