Fate of polyphenols in pili (Canarium ovatum Engl.) pomace after in vitro simulated digestion

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Elizabeth Hashim Arenas, Trinidad Palad Trinidad
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2017
• Subjects: Antioxidant; In vitro digestion; Phenolics; Bioavailability; Canarium ovatum; Pili; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2016.11.002; https://doaj.org/article/c99251a1a362455496d8ef47dc23b207

Objective: To evaluate the stability and bioavailability of polyphenols in pili (Canarium ovatum Engl.) pomace during simulated in vitro digestion. Methods: Freeze-dried pili pomace was subjected to in vitro digestion simulating conditions in the stomach, small intestine and colon. Total polyphenols, anthocyanins, flavonoids and condensed tannins, and its antioxidant activity – 1,1-diphenyl-2-picrylhydrazyl, 2,2′-azinobis-3-ethylbenzothiozoline-6-sulfonic acid, and ferric reducing antioxidant power were measured using standard spectrophotometric methods. Results: In vitro digestion of pili pomace resulted in reduction of phenolic compounds. Condensed tannins and anthocyanins were released in the gastric and intestinal stages, while total polyphenols and flavonoids after fermentation simulating colonic conditions. Antioxidant values of the bioavailable fractions showed that more than 90% of activity was lost during simulated digestion. Conclusions: Findings indicate that pili pomace is a promising functional ingredient for food and dietary supplements which can furnish potentially bioavailable phenolic antioxidants to the body.