Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae mosquitoes after feeding
Abstract Background The male mosquito microbiome may be important for identifying ideal candidates for disease control. Among other criteria, mosquito-associated symbionts that have high localization in both male and female mosquitoes and are transmissible through both vertical and sexual routes are...
Published in: | Malaria Journal |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2024
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-024-04870-w https://doaj.org/article/c991a628ef604d9dab96ef63c7f607fe |
id |
ftdoajarticles:oai:doaj.org/article:c991a628ef604d9dab96ef63c7f607fe |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:c991a628ef604d9dab96ef63c7f607fe 2024-09-09T19:28:12+00:00 Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae mosquitoes after feeding Richardson K. Egyirifa Jewelna Akorli 2024-02-01T00:00:00Z https://doi.org/10.1186/s12936-024-04870-w https://doaj.org/article/c991a628ef604d9dab96ef63c7f607fe EN eng BMC https://doi.org/10.1186/s12936-024-04870-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-024-04870-w 1475-2875 https://doaj.org/article/c991a628ef604d9dab96ef63c7f607fe Malaria Journal, Vol 23, Iss 1, Pp 1-8 (2024) Mosquito microbiota Anopheles gambiae Asaia siamensis Elizabethkingia meningoseptica Bio-control Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2024 ftdoajarticles https://doi.org/10.1186/s12936-024-04870-w 2024-08-05T17:49:53Z Abstract Background The male mosquito microbiome may be important for identifying ideal candidates for disease control. Among other criteria, mosquito-associated symbionts that have high localization in both male and female mosquitoes and are transmissible through both vertical and sexual routes are desirable. However, mosquito microbiome studies have mainly been female-focused. In this study, the microbiota of male and female Anopheles gambiae sensu lato (s.l.) were compared to identify shared or unique bacteria. Methods Late larval instars of Anopheles mosquitoes were collected from the field and raised to adults. Equal numbers of males and females of 1-day-old non-sugar-fed, 4–5-day-old sugar-fed and post-blood-fed females were randomly selected for whole-body analyses of bacteria 16S rRNA. Results Results revealed that male and female mosquitoes generally share similar microbiota except when females were blood-fed. Compared to newly emerged unfed mosquitoes, feeding on sugar and/or blood increased variability in microbial composition (⍺-diversity), with a higher disparity among females (39% P = 0.01) than in males (29% P = 0.03). Elizabethkingia meningoseptica and Asaia siamensis were common discriminants between feeding statuses in both males and females. While E. meningoseptica was particularly associated with sugar-fed mosquitoes of both sexes and sustained after blood feeding in females, A. siamensis was also increased in sugar-fed mosquitoes but decreased significantly in blood-fed females (LDA score > 4.0, P < 0.05). Among males, A. siamensis did not differ significantly after sugar meals. Conclusions Results indicate the opportunities for stable infection in mosquitoes should these species be used in bacteria-mediated disease control. Further studies are recommended to investigate possible host-specific tissue tropism of bacteria species which will inform selection of the most appropriate microbes for effective transmission-blocking strategies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 23 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Mosquito microbiota Anopheles gambiae Asaia siamensis Elizabethkingia meningoseptica Bio-control Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Mosquito microbiota Anopheles gambiae Asaia siamensis Elizabethkingia meningoseptica Bio-control Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Richardson K. Egyirifa Jewelna Akorli Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae mosquitoes after feeding |
topic_facet |
Mosquito microbiota Anopheles gambiae Asaia siamensis Elizabethkingia meningoseptica Bio-control Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The male mosquito microbiome may be important for identifying ideal candidates for disease control. Among other criteria, mosquito-associated symbionts that have high localization in both male and female mosquitoes and are transmissible through both vertical and sexual routes are desirable. However, mosquito microbiome studies have mainly been female-focused. In this study, the microbiota of male and female Anopheles gambiae sensu lato (s.l.) were compared to identify shared or unique bacteria. Methods Late larval instars of Anopheles mosquitoes were collected from the field and raised to adults. Equal numbers of males and females of 1-day-old non-sugar-fed, 4–5-day-old sugar-fed and post-blood-fed females were randomly selected for whole-body analyses of bacteria 16S rRNA. Results Results revealed that male and female mosquitoes generally share similar microbiota except when females were blood-fed. Compared to newly emerged unfed mosquitoes, feeding on sugar and/or blood increased variability in microbial composition (⍺-diversity), with a higher disparity among females (39% P = 0.01) than in males (29% P = 0.03). Elizabethkingia meningoseptica and Asaia siamensis were common discriminants between feeding statuses in both males and females. While E. meningoseptica was particularly associated with sugar-fed mosquitoes of both sexes and sustained after blood feeding in females, A. siamensis was also increased in sugar-fed mosquitoes but decreased significantly in blood-fed females (LDA score > 4.0, P < 0.05). Among males, A. siamensis did not differ significantly after sugar meals. Conclusions Results indicate the opportunities for stable infection in mosquitoes should these species be used in bacteria-mediated disease control. Further studies are recommended to investigate possible host-specific tissue tropism of bacteria species which will inform selection of the most appropriate microbes for effective transmission-blocking strategies. |
format |
Article in Journal/Newspaper |
author |
Richardson K. Egyirifa Jewelna Akorli |
author_facet |
Richardson K. Egyirifa Jewelna Akorli |
author_sort |
Richardson K. Egyirifa |
title |
Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae mosquitoes after feeding |
title_short |
Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae mosquitoes after feeding |
title_full |
Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae mosquitoes after feeding |
title_fullStr |
Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae mosquitoes after feeding |
title_full_unstemmed |
Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae mosquitoes after feeding |
title_sort |
two promising candidates for paratransgenesis, elizabethkingia and asaia, increase in both sexes of anopheles gambiae mosquitoes after feeding |
publisher |
BMC |
publishDate |
2024 |
url |
https://doi.org/10.1186/s12936-024-04870-w https://doaj.org/article/c991a628ef604d9dab96ef63c7f607fe |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 23, Iss 1, Pp 1-8 (2024) |
op_relation |
https://doi.org/10.1186/s12936-024-04870-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-024-04870-w 1475-2875 https://doaj.org/article/c991a628ef604d9dab96ef63c7f607fe |
op_doi |
https://doi.org/10.1186/s12936-024-04870-w |
container_title |
Malaria Journal |
container_volume |
23 |
container_issue |
1 |
_version_ |
1809897465756778496 |