Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine ( Canis lupus familiaris ) Epididymal Spermatozoa
Increased male age is associated with a significant reduction in semen quality. Little is known about the sperm proteome changes resulting from the aging process. This study aimed to investigate the relationship between the functional quality and proteome of epididymal spermatozoa of dogs that were...
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ftdoajarticles:oai:doaj.org/article:c96fc9bdf270476db373debce223b627 2024-01-07T09:42:35+01:00 Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine ( Canis lupus familiaris ) Epididymal Spermatozoa Anna Zmudzinska Jerzy Wisniewski Piotr Mlynarz Beata Olejnik Marzena Mogielnicka-Brzozowska 2022-08-01T00:00:00Z https://doi.org/10.3390/ijms23169143 https://doaj.org/article/c96fc9bdf270476db373debce223b627 EN eng MDPI AG https://www.mdpi.com/1422-0067/23/16/9143 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms23169143 1422-0067 1661-6596 https://doaj.org/article/c96fc9bdf270476db373debce223b627 International Journal of Molecular Sciences, Vol 23, Iss 16, p 9143 (2022) epididymal spermatozoa aging proteins canine Biology (General) QH301-705.5 Chemistry QD1-999 article 2022 ftdoajarticles https://doi.org/10.3390/ijms23169143 2023-12-10T01:44:42Z Increased male age is associated with a significant reduction in semen quality. Little is known about the sperm proteome changes resulting from the aging process. This study aimed to investigate the relationship between the functional quality and proteome of epididymal spermatozoa of dogs that were differing in age. The study was conducted on 30 male dogs that were divided into three age groups. G1—12 to 41 months old, G2—42 to 77 months old, and G3—78 to 132 months old. The sperm samples were assessed using a computer-assisted semen analysis (CASA). The epididymal sperm proteins were analyzed using gel electrophoresis (SDS-PAGE), nano-liquid chromatography coupled to quadrupole time of flight mass spectrometry (NanoUPLC-Q-TOF/MS) and bioinformatic tools. The sperm quality parameters were significantly lower in older dogs. NanoUPLC-Q-TOF/MS identification resulted in 865 proteins that were found in the G1, 472 in G2, and 435 in G3. There were seven proteins that were present in all three age groups, and four of them (ACTB, CE10, NPC2, CRISP2) showed significant changes among the studied groups. Age-dependent variations were detected in the sperm proteome composition and were related to important metabolite pathways, which might suggest that several proteins are implicated in sperm maturation and could be potential aging biomarkers. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 23 16 9143 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
epididymal spermatozoa aging proteins canine Biology (General) QH301-705.5 Chemistry QD1-999 |
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epididymal spermatozoa aging proteins canine Biology (General) QH301-705.5 Chemistry QD1-999 Anna Zmudzinska Jerzy Wisniewski Piotr Mlynarz Beata Olejnik Marzena Mogielnicka-Brzozowska Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine ( Canis lupus familiaris ) Epididymal Spermatozoa |
topic_facet |
epididymal spermatozoa aging proteins canine Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Increased male age is associated with a significant reduction in semen quality. Little is known about the sperm proteome changes resulting from the aging process. This study aimed to investigate the relationship between the functional quality and proteome of epididymal spermatozoa of dogs that were differing in age. The study was conducted on 30 male dogs that were divided into three age groups. G1—12 to 41 months old, G2—42 to 77 months old, and G3—78 to 132 months old. The sperm samples were assessed using a computer-assisted semen analysis (CASA). The epididymal sperm proteins were analyzed using gel electrophoresis (SDS-PAGE), nano-liquid chromatography coupled to quadrupole time of flight mass spectrometry (NanoUPLC-Q-TOF/MS) and bioinformatic tools. The sperm quality parameters were significantly lower in older dogs. NanoUPLC-Q-TOF/MS identification resulted in 865 proteins that were found in the G1, 472 in G2, and 435 in G3. There were seven proteins that were present in all three age groups, and four of them (ACTB, CE10, NPC2, CRISP2) showed significant changes among the studied groups. Age-dependent variations were detected in the sperm proteome composition and were related to important metabolite pathways, which might suggest that several proteins are implicated in sperm maturation and could be potential aging biomarkers. |
format |
Article in Journal/Newspaper |
author |
Anna Zmudzinska Jerzy Wisniewski Piotr Mlynarz Beata Olejnik Marzena Mogielnicka-Brzozowska |
author_facet |
Anna Zmudzinska Jerzy Wisniewski Piotr Mlynarz Beata Olejnik Marzena Mogielnicka-Brzozowska |
author_sort |
Anna Zmudzinska |
title |
Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine ( Canis lupus familiaris ) Epididymal Spermatozoa |
title_short |
Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine ( Canis lupus familiaris ) Epididymal Spermatozoa |
title_full |
Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine ( Canis lupus familiaris ) Epididymal Spermatozoa |
title_fullStr |
Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine ( Canis lupus familiaris ) Epididymal Spermatozoa |
title_full_unstemmed |
Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine ( Canis lupus familiaris ) Epididymal Spermatozoa |
title_sort |
age-dependent variations in functional quality and proteomic characteristics of canine ( canis lupus familiaris ) epididymal spermatozoa |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms23169143 https://doaj.org/article/c96fc9bdf270476db373debce223b627 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
International Journal of Molecular Sciences, Vol 23, Iss 16, p 9143 (2022) |
op_relation |
https://www.mdpi.com/1422-0067/23/16/9143 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms23169143 1422-0067 1661-6596 https://doaj.org/article/c96fc9bdf270476db373debce223b627 |
op_doi |
https://doi.org/10.3390/ijms23169143 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
16 |
container_start_page |
9143 |
_version_ |
1787423620416405504 |