Reproduction of Leishmania (Leishmania ) infantum chagasi in conditioned cell culture growth medium Isolamento e crescimento de Leishmania (Leishmania) infantum chagasi em meio de crescimento condicionado de cultura celular

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Yeda L. Nogueira, Eunice A.B. Galati
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 2006
• Subjects: Leishmania (Leishmania) infantum chagasi; Flagellate form; Growth; McCoy Cell Lineage; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic; McCoy
• Online Access: https://doi.org/10.1590/S0036-46652006000300006; https://doaj.org/article/c956659f8d964500abf3e92f147a1643

Leishmanias can be produced by inoculation in conditioned McCoy cell culture growth medium (CGM). Leishmania (Leishmania) infantum chagasi (100 parasites) grown in NNN medium was inoculated in 2.5 mL CGM, kept in plates (24 wells) and its multiplication was observed for five days (120 hours). After...