Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil
Abstract Background The naturally-acquired immune response to Plasmodium vivax variant antigens (VIR) was evaluated in individuals exposed to malaria and living in different endemic areas for malaria in the north of Brazil. Methods Seven recombinant proteins representing four vir subfamilies (A, B,...
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ftdoajarticles:oai:doaj.org/article:c91805dfb4a744dfa70f518267f15a7f 2023-05-15T15:10:43+02:00 Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil Soares Irene S Del Portillo Hernando A Jimenez Maria Carolina S Fernandez-Becerra Carmen Oliveira Tatiane R 2006-10-01T00:00:00Z https://doi.org/10.1186/1475-2875-5-83 https://doaj.org/article/c91805dfb4a744dfa70f518267f15a7f EN eng BMC http://www.malariajournal.com/content/5/1/83 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-83 1475-2875 https://doaj.org/article/c91805dfb4a744dfa70f518267f15a7f Malaria Journal, Vol 5, Iss 1, p 83 (2006) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2006 ftdoajarticles https://doi.org/10.1186/1475-2875-5-83 2022-12-31T08:44:04Z Abstract Background The naturally-acquired immune response to Plasmodium vivax variant antigens (VIR) was evaluated in individuals exposed to malaria and living in different endemic areas for malaria in the north of Brazil. Methods Seven recombinant proteins representing four vir subfamilies (A, B, C, and E) obtained from a single patient from the Amazon Region were expressed in Escherichia coli as soluble glutathione S-transferase fusion proteins. The different recombinant proteins were compared by ELISA with regard to the recognition by IgM, IgG, and IgG subclass of antibodies from 200 individuals with patent infection. Results The frequency of individuals that presented antibodies anti-VIR (IgM plus IgG) during the infection was 49%. The frequencies of individuals that presented IgM or IgG antibodies anti-VIR were 29.6% or 26.0%, respectively. The prevalence of IgG antibodies against recombinant VIR proteins was significantly lower than the prevalence of antibodies against the recombinant proteins representing two surface antigens of merozoites of P. vivax : AMA-1 and MSP1 19 (57.0% and 90.5%, respectively). The cellular immune response to VIR antigens was evaluated by in vitro proliferative assays in mononuclear cells of the individuals recently exposed to P. vivax . No significant proliferative response to these antigens was observed when comparing malaria-exposed to non-exposed individuals. Conclusion This study provides evidence that there is a low frequency of individuals responding to each VIR antigens in endemic areas of Brazil. This fact may explain the host susceptibility to new episodes of the disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 5 1 83 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Soares Irene S Del Portillo Hernando A Jimenez Maria Carolina S Fernandez-Becerra Carmen Oliveira Tatiane R Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The naturally-acquired immune response to Plasmodium vivax variant antigens (VIR) was evaluated in individuals exposed to malaria and living in different endemic areas for malaria in the north of Brazil. Methods Seven recombinant proteins representing four vir subfamilies (A, B, C, and E) obtained from a single patient from the Amazon Region were expressed in Escherichia coli as soluble glutathione S-transferase fusion proteins. The different recombinant proteins were compared by ELISA with regard to the recognition by IgM, IgG, and IgG subclass of antibodies from 200 individuals with patent infection. Results The frequency of individuals that presented antibodies anti-VIR (IgM plus IgG) during the infection was 49%. The frequencies of individuals that presented IgM or IgG antibodies anti-VIR were 29.6% or 26.0%, respectively. The prevalence of IgG antibodies against recombinant VIR proteins was significantly lower than the prevalence of antibodies against the recombinant proteins representing two surface antigens of merozoites of P. vivax : AMA-1 and MSP1 19 (57.0% and 90.5%, respectively). The cellular immune response to VIR antigens was evaluated by in vitro proliferative assays in mononuclear cells of the individuals recently exposed to P. vivax . No significant proliferative response to these antigens was observed when comparing malaria-exposed to non-exposed individuals. Conclusion This study provides evidence that there is a low frequency of individuals responding to each VIR antigens in endemic areas of Brazil. This fact may explain the host susceptibility to new episodes of the disease. |
format |
Article in Journal/Newspaper |
author |
Soares Irene S Del Portillo Hernando A Jimenez Maria Carolina S Fernandez-Becerra Carmen Oliveira Tatiane R |
author_facet |
Soares Irene S Del Portillo Hernando A Jimenez Maria Carolina S Fernandez-Becerra Carmen Oliveira Tatiane R |
author_sort |
Soares Irene S |
title |
Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil |
title_short |
Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil |
title_full |
Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil |
title_fullStr |
Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil |
title_full_unstemmed |
Evaluation of the acquired immune responses to Plasmodium vivax VIR variant antigens in individuals living in malaria-endemic areas of Brazil |
title_sort |
evaluation of the acquired immune responses to plasmodium vivax vir variant antigens in individuals living in malaria-endemic areas of brazil |
publisher |
BMC |
publishDate |
2006 |
url |
https://doi.org/10.1186/1475-2875-5-83 https://doaj.org/article/c91805dfb4a744dfa70f518267f15a7f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
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Arctic |
op_source |
Malaria Journal, Vol 5, Iss 1, p 83 (2006) |
op_relation |
http://www.malariajournal.com/content/5/1/83 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-83 1475-2875 https://doaj.org/article/c91805dfb4a744dfa70f518267f15a7f |
op_doi |
https://doi.org/10.1186/1475-2875-5-83 |
container_title |
Malaria Journal |
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5 |
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1 |
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83 |
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