Localization and Bioreactivity of Cysteine-Rich Secretions in the Marine Gastropod Nucella lapillus
Marine biodiversity has been yielding promising novel bioproducts from venomous animals. Despite the auspices of conotoxins, which originated the paradigmatic painkiller Prialt, the biotechnological potential of gastropod venoms remains to be explored. Marine bioprospecting is expanding towards temp...
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MDPI AG
2021
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ftdoajarticles:oai:doaj.org/article:c8bc3b22e3854a38bd82cbc1a247b6a3 2023-05-15T18:49:53+02:00 Localization and Bioreactivity of Cysteine-Rich Secretions in the Marine Gastropod Nucella lapillus Mariaelena D’Ambrosio Cátia Gonçalves Mariana Calmão Maria Rodrigues Pedro M. Costa 2021-05-01T00:00:00Z https://doi.org/10.3390/md19050276 https://doaj.org/article/c8bc3b22e3854a38bd82cbc1a247b6a3 EN eng MDPI AG https://www.mdpi.com/1660-3397/19/5/276 https://doaj.org/toc/1660-3397 doi:10.3390/md19050276 1660-3397 https://doaj.org/article/c8bc3b22e3854a38bd82cbc1a247b6a3 Marine Drugs, Vol 19, Iss 276, p 276 (2021) marine biotechnology bioreactives toxicity thiols dogwhelk Gastropoda Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/md19050276 2022-12-30T23:49:34Z Marine biodiversity has been yielding promising novel bioproducts from venomous animals. Despite the auspices of conotoxins, which originated the paradigmatic painkiller Prialt, the biotechnological potential of gastropod venoms remains to be explored. Marine bioprospecting is expanding towards temperate species like the dogwhelk Nucella lapillus , which is suspected to secrete immobilizing agents through its salivary glands with a relaxing effect on the musculature of its preferential prey, Mytilus sp. This work focused on detecting, localizing, and testing the bioreactivity of cysteine-rich proteins and peptides, whose presence is a signature of animal venoms and poisons. The highest content of thiols was found in crude protein extracts from the digestive gland, which is associated with digestion, followed by the peribuccal mass, where the salivary glands are located. Conversely, the foot and siphon (which the gastropod uses for feeding) are not the main organs involved in toxin secretion. Ex vivo bioassays with Mytilus gill tissue disclosed the differential bioreactivity of crude protein extracts. Secretions from the digestive gland and peribuccal mass caused the most significant molecular damage, with evidence for the induction of apoptosis. These early findings indicate that salivary glands are a promising target for the extraction and characterization of bioactive cysteine-rich proteinaceous toxins from the species. Article in Journal/Newspaper Dogwhelk Nucella lapillus Directory of Open Access Journals: DOAJ Articles Marine Drugs 19 5 276 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
marine biotechnology bioreactives toxicity thiols dogwhelk Gastropoda Biology (General) QH301-705.5 |
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marine biotechnology bioreactives toxicity thiols dogwhelk Gastropoda Biology (General) QH301-705.5 Mariaelena D’Ambrosio Cátia Gonçalves Mariana Calmão Maria Rodrigues Pedro M. Costa Localization and Bioreactivity of Cysteine-Rich Secretions in the Marine Gastropod Nucella lapillus |
topic_facet |
marine biotechnology bioreactives toxicity thiols dogwhelk Gastropoda Biology (General) QH301-705.5 |
description |
Marine biodiversity has been yielding promising novel bioproducts from venomous animals. Despite the auspices of conotoxins, which originated the paradigmatic painkiller Prialt, the biotechnological potential of gastropod venoms remains to be explored. Marine bioprospecting is expanding towards temperate species like the dogwhelk Nucella lapillus , which is suspected to secrete immobilizing agents through its salivary glands with a relaxing effect on the musculature of its preferential prey, Mytilus sp. This work focused on detecting, localizing, and testing the bioreactivity of cysteine-rich proteins and peptides, whose presence is a signature of animal venoms and poisons. The highest content of thiols was found in crude protein extracts from the digestive gland, which is associated with digestion, followed by the peribuccal mass, where the salivary glands are located. Conversely, the foot and siphon (which the gastropod uses for feeding) are not the main organs involved in toxin secretion. Ex vivo bioassays with Mytilus gill tissue disclosed the differential bioreactivity of crude protein extracts. Secretions from the digestive gland and peribuccal mass caused the most significant molecular damage, with evidence for the induction of apoptosis. These early findings indicate that salivary glands are a promising target for the extraction and characterization of bioactive cysteine-rich proteinaceous toxins from the species. |
format |
Article in Journal/Newspaper |
author |
Mariaelena D’Ambrosio Cátia Gonçalves Mariana Calmão Maria Rodrigues Pedro M. Costa |
author_facet |
Mariaelena D’Ambrosio Cátia Gonçalves Mariana Calmão Maria Rodrigues Pedro M. Costa |
author_sort |
Mariaelena D’Ambrosio |
title |
Localization and Bioreactivity of Cysteine-Rich Secretions in the Marine Gastropod Nucella lapillus |
title_short |
Localization and Bioreactivity of Cysteine-Rich Secretions in the Marine Gastropod Nucella lapillus |
title_full |
Localization and Bioreactivity of Cysteine-Rich Secretions in the Marine Gastropod Nucella lapillus |
title_fullStr |
Localization and Bioreactivity of Cysteine-Rich Secretions in the Marine Gastropod Nucella lapillus |
title_full_unstemmed |
Localization and Bioreactivity of Cysteine-Rich Secretions in the Marine Gastropod Nucella lapillus |
title_sort |
localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/md19050276 https://doaj.org/article/c8bc3b22e3854a38bd82cbc1a247b6a3 |
genre |
Dogwhelk Nucella lapillus |
genre_facet |
Dogwhelk Nucella lapillus |
op_source |
Marine Drugs, Vol 19, Iss 276, p 276 (2021) |
op_relation |
https://www.mdpi.com/1660-3397/19/5/276 https://doaj.org/toc/1660-3397 doi:10.3390/md19050276 1660-3397 https://doaj.org/article/c8bc3b22e3854a38bd82cbc1a247b6a3 |
op_doi |
https://doi.org/10.3390/md19050276 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
5 |
container_start_page |
276 |
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1766243499225645056 |