Insulin reduces the requirement for serum in Plasmodium falciparum culture
Insulin added to Plasmodium falciparum cultures (0.2 IU/ml) reduced the requirement for human serum from ten to five percent. This represents an obvious advantage by its serum-sparing effect and by reducing the chances of using contaminated serum in cultures. The growth-promoting ability of insulin...
| Main Authors: | , |
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| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
1984
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| Subjects: | |
| Online Access: | https://doaj.org/article/c8a43884686f46cd8261b5590a0d6251 |
| Summary: | Insulin added to Plasmodium falciparum cultures (0.2 IU/ml) reduced the requirement for human serum from ten to five percent. This represents an obvious advantage by its serum-sparing effect and by reducing the chances of using contaminated serum in cultures. The growth-promoting ability of insulin was observed eitherin culture- adapted P. falciparum or in newly-isolated samples. A adição de insulina (0,2) UI/ml) à cultura de Plasmodium falciparum reduziu a necessidade de soro humano de dez para cinco por cento. Isto representa uma óbvia vantagem não só pela economia de soro como pela diminuição do risco de se utilizar amostras de soro contaminadas nas culturas. Esta capacidade da insulina de promover a multiplicação de plasmódio foi observada tanto em relação à P. falciparum adaptado à cultura por 12 meses como em amostras recém-isoladas do parasito. |
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