Comparison of phylogeny, venom composition and neutralization by antivenom in diverse species of bothrops complex.
In Latin America, Bothrops snakes account for most snake bites in humans, and the recommended treatment is administration of multispecific Bothrops antivenom (SAB--soro antibotrópico). However, Bothrops snakes are very diverse with regard to their venom composition, which raises the issue of which v...
Published in: | PLoS Neglected Tropical Diseases |
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2013
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Online Access: | https://doi.org/10.1371/journal.pntd.0002442 https://doaj.org/article/c881e9b5ad7b4cecba8796c5c078211c |
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ftdoajarticles:oai:doaj.org/article:c881e9b5ad7b4cecba8796c5c078211c 2023-05-15T15:14:21+02:00 Comparison of phylogeny, venom composition and neutralization by antivenom in diverse species of bothrops complex. Leijiane F Sousa Carolina A Nicolau Pedro S Peixoto Juliana L Bernardoni Sâmella S Oliveira José Antonio Portes-Junior Rosa Helena V Mourão Isa Lima-dos-Santos Ida S Sano-Martins Hipócrates M Chalkidis Richard H Valente Ana M Moura-da-Silva 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002442 https://doaj.org/article/c881e9b5ad7b4cecba8796c5c078211c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3772048?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002442 https://doaj.org/article/c881e9b5ad7b4cecba8796c5c078211c PLoS Neglected Tropical Diseases, Vol 7, Iss 9, p e2442 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002442 2022-12-31T14:27:51Z In Latin America, Bothrops snakes account for most snake bites in humans, and the recommended treatment is administration of multispecific Bothrops antivenom (SAB--soro antibotrópico). However, Bothrops snakes are very diverse with regard to their venom composition, which raises the issue of which venoms should be used as immunizing antigens for the production of pan-specific Bothrops antivenoms. In this study, we simultaneously compared the composition and reactivity with SAB of venoms collected from six species of snakes, distributed in pairs from three distinct phylogenetic clades: Bothrops, Bothropoides and Rhinocerophis. We also evaluated the neutralization of Bothrops atrox venom, which is the species responsible for most snake bites in the Amazon region, but not included in the immunization antigen mixture used to produce SAB. Using mass spectrometric and chromatographic approaches, we observed a lack of similarity in protein composition between the venoms from closely related snakes and a high similarity between the venoms of phylogenetically more distant snakes, suggesting little connection between taxonomic position and venom composition. P-III snake venom metalloproteinases (SVMPs) are the most antigenic toxins in the venoms of snakes from the Bothrops complex, whereas class P-I SVMPs, snake venom serine proteinases and phospholipases A2 reacted with antibodies in lower levels. Low molecular size toxins, such as disintegrins and bradykinin-potentiating peptides, were poorly antigenic. Toxins from the same protein family showed antigenic cross-reactivity among venoms from different species; SAB was efficient in neutralizing the B. atrox venom major toxins. Thus, we suggest that it is possible to obtain pan-specific effective antivenoms for Bothrops envenomations through immunization with venoms from only a few species of snakes, if these venoms contain protein classes that are representative of all species to which the antivenom is targeted. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 9 e2442 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Leijiane F Sousa Carolina A Nicolau Pedro S Peixoto Juliana L Bernardoni Sâmella S Oliveira José Antonio Portes-Junior Rosa Helena V Mourão Isa Lima-dos-Santos Ida S Sano-Martins Hipócrates M Chalkidis Richard H Valente Ana M Moura-da-Silva Comparison of phylogeny, venom composition and neutralization by antivenom in diverse species of bothrops complex. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
In Latin America, Bothrops snakes account for most snake bites in humans, and the recommended treatment is administration of multispecific Bothrops antivenom (SAB--soro antibotrópico). However, Bothrops snakes are very diverse with regard to their venom composition, which raises the issue of which venoms should be used as immunizing antigens for the production of pan-specific Bothrops antivenoms. In this study, we simultaneously compared the composition and reactivity with SAB of venoms collected from six species of snakes, distributed in pairs from three distinct phylogenetic clades: Bothrops, Bothropoides and Rhinocerophis. We also evaluated the neutralization of Bothrops atrox venom, which is the species responsible for most snake bites in the Amazon region, but not included in the immunization antigen mixture used to produce SAB. Using mass spectrometric and chromatographic approaches, we observed a lack of similarity in protein composition between the venoms from closely related snakes and a high similarity between the venoms of phylogenetically more distant snakes, suggesting little connection between taxonomic position and venom composition. P-III snake venom metalloproteinases (SVMPs) are the most antigenic toxins in the venoms of snakes from the Bothrops complex, whereas class P-I SVMPs, snake venom serine proteinases and phospholipases A2 reacted with antibodies in lower levels. Low molecular size toxins, such as disintegrins and bradykinin-potentiating peptides, were poorly antigenic. Toxins from the same protein family showed antigenic cross-reactivity among venoms from different species; SAB was efficient in neutralizing the B. atrox venom major toxins. Thus, we suggest that it is possible to obtain pan-specific effective antivenoms for Bothrops envenomations through immunization with venoms from only a few species of snakes, if these venoms contain protein classes that are representative of all species to which the antivenom is targeted. |
format |
Article in Journal/Newspaper |
author |
Leijiane F Sousa Carolina A Nicolau Pedro S Peixoto Juliana L Bernardoni Sâmella S Oliveira José Antonio Portes-Junior Rosa Helena V Mourão Isa Lima-dos-Santos Ida S Sano-Martins Hipócrates M Chalkidis Richard H Valente Ana M Moura-da-Silva |
author_facet |
Leijiane F Sousa Carolina A Nicolau Pedro S Peixoto Juliana L Bernardoni Sâmella S Oliveira José Antonio Portes-Junior Rosa Helena V Mourão Isa Lima-dos-Santos Ida S Sano-Martins Hipócrates M Chalkidis Richard H Valente Ana M Moura-da-Silva |
author_sort |
Leijiane F Sousa |
title |
Comparison of phylogeny, venom composition and neutralization by antivenom in diverse species of bothrops complex. |
title_short |
Comparison of phylogeny, venom composition and neutralization by antivenom in diverse species of bothrops complex. |
title_full |
Comparison of phylogeny, venom composition and neutralization by antivenom in diverse species of bothrops complex. |
title_fullStr |
Comparison of phylogeny, venom composition and neutralization by antivenom in diverse species of bothrops complex. |
title_full_unstemmed |
Comparison of phylogeny, venom composition and neutralization by antivenom in diverse species of bothrops complex. |
title_sort |
comparison of phylogeny, venom composition and neutralization by antivenom in diverse species of bothrops complex. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002442 https://doaj.org/article/c881e9b5ad7b4cecba8796c5c078211c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 9, p e2442 (2013) |
op_relation |
http://europepmc.org/articles/PMC3772048?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002442 https://doaj.org/article/c881e9b5ad7b4cecba8796c5c078211c |
op_doi |
https://doi.org/10.1371/journal.pntd.0002442 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
9 |
container_start_page |
e2442 |
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1766344819988234240 |