A novel in vitro model reveals distinctive modulatory roles of Plasmodium falciparum and Plasmodium vivax on naïve cell-mediated immunity
Abstract Background To date, human peripheral blood mononuclear cells (PBMCs) have been used mainly in immune stimulation assays and the interpretation of data can be influenced by the previous immunological history of donors and cross reactivity with other infectious agents. Resolving these limitat...
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ftdoajarticles:oai:doaj.org/article:c86eb180dc35470f82df4a5fc7da16b7 2023-05-15T15:11:49+02:00 A novel in vitro model reveals distinctive modulatory roles of Plasmodium falciparum and Plasmodium vivax on naïve cell-mediated immunity Setthakit Chitsanoor Sangdao Somsri Panyu Panburana Mathirut Mungthin Ratawan Ubalee Maliwan Emyeam Somchai Jongwutiwes Jetsumon Sattabongkot Rachanee Udomsangpetch 2017-03-01T00:00:00Z https://doi.org/10.1186/s12936-017-1781-4 https://doaj.org/article/c86eb180dc35470f82df4a5fc7da16b7 EN eng BMC http://link.springer.com/article/10.1186/s12936-017-1781-4 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1781-4 1475-2875 https://doaj.org/article/c86eb180dc35470f82df4a5fc7da16b7 Malaria Journal, Vol 16, Iss 1, Pp 1-10 (2017) Hematopoietic stem cells Cell mediated immunity Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-1781-4 2022-12-31T11:26:27Z Abstract Background To date, human peripheral blood mononuclear cells (PBMCs) have been used mainly in immune stimulation assays and the interpretation of data can be influenced by the previous immunological history of donors and cross reactivity with other infectious agents. Resolving these limitations requires an alternative in vitro model to uncover the primary response profiles. Methods A novel in vitro model of mononuclear cells (MNCs) generated from haematopoietic stem cells (HSCs) was developed and these cells were then co-cultured with various antigens from Plasmodium falciparum and Plasmodium vivax to investigate the response of naïve immune cells to malaria antigens by flow cytometry. Results In vitro stimulation of naïve lymphocytes showed that CD4+ and CD8+ T lymphocytes were significantly reduced (P < 0.01) by exposure to lysates of infected erythrocytes or intact erythrocytes infected with P. falciparum. The depletion was associated with the expression of CD95 (Fas receptor) on the surface of T lymphocytes. Maturation of T lymphocytes was affected differently, showing elevated CD3+CD4+CD8+ and CD3+CD4−CD8− T lymphocytes after stimulation with cell lysates of P. falciparum and P. vivax, respectively. In addition, antigen presenting monocytes and dendritic cells derived from haematopoietic stem cells showed impaired HLA-DR expression as a consequence of exposure to different species of malaria parasites. Conclusion These results suggest that naïve mononuclear cells differentiated in vitro from HSCs could provide a valid model for the assessment of immunity. P. falciparum and P. vivax malaria parasites could modulate various populations of immune cells starting from newly differentiated mononuclear cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Hematopoietic stem cells Cell mediated immunity Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Hematopoietic stem cells Cell mediated immunity Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Setthakit Chitsanoor Sangdao Somsri Panyu Panburana Mathirut Mungthin Ratawan Ubalee Maliwan Emyeam Somchai Jongwutiwes Jetsumon Sattabongkot Rachanee Udomsangpetch A novel in vitro model reveals distinctive modulatory roles of Plasmodium falciparum and Plasmodium vivax on naïve cell-mediated immunity |
topic_facet |
Hematopoietic stem cells Cell mediated immunity Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background To date, human peripheral blood mononuclear cells (PBMCs) have been used mainly in immune stimulation assays and the interpretation of data can be influenced by the previous immunological history of donors and cross reactivity with other infectious agents. Resolving these limitations requires an alternative in vitro model to uncover the primary response profiles. Methods A novel in vitro model of mononuclear cells (MNCs) generated from haematopoietic stem cells (HSCs) was developed and these cells were then co-cultured with various antigens from Plasmodium falciparum and Plasmodium vivax to investigate the response of naïve immune cells to malaria antigens by flow cytometry. Results In vitro stimulation of naïve lymphocytes showed that CD4+ and CD8+ T lymphocytes were significantly reduced (P < 0.01) by exposure to lysates of infected erythrocytes or intact erythrocytes infected with P. falciparum. The depletion was associated with the expression of CD95 (Fas receptor) on the surface of T lymphocytes. Maturation of T lymphocytes was affected differently, showing elevated CD3+CD4+CD8+ and CD3+CD4−CD8− T lymphocytes after stimulation with cell lysates of P. falciparum and P. vivax, respectively. In addition, antigen presenting monocytes and dendritic cells derived from haematopoietic stem cells showed impaired HLA-DR expression as a consequence of exposure to different species of malaria parasites. Conclusion These results suggest that naïve mononuclear cells differentiated in vitro from HSCs could provide a valid model for the assessment of immunity. P. falciparum and P. vivax malaria parasites could modulate various populations of immune cells starting from newly differentiated mononuclear cells. |
format |
Article in Journal/Newspaper |
author |
Setthakit Chitsanoor Sangdao Somsri Panyu Panburana Mathirut Mungthin Ratawan Ubalee Maliwan Emyeam Somchai Jongwutiwes Jetsumon Sattabongkot Rachanee Udomsangpetch |
author_facet |
Setthakit Chitsanoor Sangdao Somsri Panyu Panburana Mathirut Mungthin Ratawan Ubalee Maliwan Emyeam Somchai Jongwutiwes Jetsumon Sattabongkot Rachanee Udomsangpetch |
author_sort |
Setthakit Chitsanoor |
title |
A novel in vitro model reveals distinctive modulatory roles of Plasmodium falciparum and Plasmodium vivax on naïve cell-mediated immunity |
title_short |
A novel in vitro model reveals distinctive modulatory roles of Plasmodium falciparum and Plasmodium vivax on naïve cell-mediated immunity |
title_full |
A novel in vitro model reveals distinctive modulatory roles of Plasmodium falciparum and Plasmodium vivax on naïve cell-mediated immunity |
title_fullStr |
A novel in vitro model reveals distinctive modulatory roles of Plasmodium falciparum and Plasmodium vivax on naïve cell-mediated immunity |
title_full_unstemmed |
A novel in vitro model reveals distinctive modulatory roles of Plasmodium falciparum and Plasmodium vivax on naïve cell-mediated immunity |
title_sort |
novel in vitro model reveals distinctive modulatory roles of plasmodium falciparum and plasmodium vivax on naïve cell-mediated immunity |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-1781-4 https://doaj.org/article/c86eb180dc35470f82df4a5fc7da16b7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-10 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-1781-4 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1781-4 1475-2875 https://doaj.org/article/c86eb180dc35470f82df4a5fc7da16b7 |
op_doi |
https://doi.org/10.1186/s12936-017-1781-4 |
container_title |
Malaria Journal |
container_volume |
16 |
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1 |
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1766342608411426816 |