Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno
The key part of creating bone material for teaching is degreasing and whitening it. However, the substances used are often dangerous and toxic. We tested and compared safer methods based on two physical variables. These are light and heat. The material for our study was 45 femurs from 23 adult domes...
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ftdoajarticles:oai:doaj.org/article:c830d53631d249389a01b4861a4c8876 2024-01-07T09:42:36+01:00 Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno Ondřej Horák Martin Pyszko Václav Páral Ondřej Šandor 2022-09-01T00:00:00Z https://doi.org/10.7717/peerj.14036 https://doaj.org/article/c830d53631d249389a01b4861a4c8876 EN eng PeerJ Inc. https://peerj.com/articles/14036.pdf https://peerj.com/articles/14036/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.14036 2167-8359 https://doaj.org/article/c830d53631d249389a01b4861a4c8876 PeerJ, Vol 10, p e14036 (2022) Bone whitening Heat and light Bone degreasing Medicine R Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.7717/peerj.14036 2023-12-10T01:51:49Z The key part of creating bone material for teaching is degreasing and whitening it. However, the substances used are often dangerous and toxic. We tested and compared safer methods based on two physical variables. These are light and heat. The material for our study was 45 femurs from 23 adult domestic dogs (Canis lupus f. familiaris). The bones were divided into three groups of 15 pieces according to the method used to remove muscles and ligaments from their surface. Five femurs from each group were exposed to three different light sources for 28 days—sunlight, warm light from a classical incandescent light bulb and cold light by a LED bulb. At regular intervals, the change in the colour of the bone surface and the amount of fat loss from the medullary cavity was also monitored. The best degreasing and bleaching results were achieved in macerated bones exposed to sunlight. They achieved the required condition as early as 21 days after the start of sun exposure. The biggest problem was haemoglobin, which permeated through the Haversian canals and discoloured the bone tissue. The results showed that the use of light and heat is a suitable and safe alternative to chemical methods of degreasing and bleaching bones. The disadvantage is the length of time, especially for native material. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles PeerJ 10 e14036 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bone whitening Heat and light Bone degreasing Medicine R Biology (General) QH301-705.5 |
spellingShingle |
Bone whitening Heat and light Bone degreasing Medicine R Biology (General) QH301-705.5 Ondřej Horák Martin Pyszko Václav Páral Ondřej Šandor Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
topic_facet |
Bone whitening Heat and light Bone degreasing Medicine R Biology (General) QH301-705.5 |
description |
The key part of creating bone material for teaching is degreasing and whitening it. However, the substances used are often dangerous and toxic. We tested and compared safer methods based on two physical variables. These are light and heat. The material for our study was 45 femurs from 23 adult domestic dogs (Canis lupus f. familiaris). The bones were divided into three groups of 15 pieces according to the method used to remove muscles and ligaments from their surface. Five femurs from each group were exposed to three different light sources for 28 days—sunlight, warm light from a classical incandescent light bulb and cold light by a LED bulb. At regular intervals, the change in the colour of the bone surface and the amount of fat loss from the medullary cavity was also monitored. The best degreasing and bleaching results were achieved in macerated bones exposed to sunlight. They achieved the required condition as early as 21 days after the start of sun exposure. The biggest problem was haemoglobin, which permeated through the Haversian canals and discoloured the bone tissue. The results showed that the use of light and heat is a suitable and safe alternative to chemical methods of degreasing and bleaching bones. The disadvantage is the length of time, especially for native material. |
format |
Article in Journal/Newspaper |
author |
Ondřej Horák Martin Pyszko Václav Páral Ondřej Šandor |
author_facet |
Ondřej Horák Martin Pyszko Václav Páral Ondřej Šandor |
author_sort |
Ondřej Horák |
title |
Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
title_short |
Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
title_full |
Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
title_fullStr |
Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
title_full_unstemmed |
Degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the University of Veterinary Sciences Brno |
title_sort |
degreasing and bleaching bones using light sources as a tool to increase the safety of teaching osteology at the university of veterinary sciences brno |
publisher |
PeerJ Inc. |
publishDate |
2022 |
url |
https://doi.org/10.7717/peerj.14036 https://doaj.org/article/c830d53631d249389a01b4861a4c8876 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
PeerJ, Vol 10, p e14036 (2022) |
op_relation |
https://peerj.com/articles/14036.pdf https://peerj.com/articles/14036/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.14036 2167-8359 https://doaj.org/article/c830d53631d249389a01b4861a4c8876 |
op_doi |
https://doi.org/10.7717/peerj.14036 |
container_title |
PeerJ |
container_volume |
10 |
container_start_page |
e14036 |
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