Biological characteristics and permissiveness to viruses of diploid kidney cells strain from the bat Nathusius’ pipistrelle ( Pipistrellus nathusii Keyserling & Blasius, 1839; Chiroptera: Microchiroptera: Vespertilionidae )
Introduction. Bats are an epidemiologically important natural reservoir of viruses of various taxonomic groups, including causative agents of especially dangerous infections of humans and animals. Considering the relevance of arbovirus infections, it seems advisable to study the spectrum of the sens...
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Central Research Institute for Epidemiology
2021
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ftdoajarticles:oai:doaj.org/article:c816e53d76234ec59d6ebbcf2a96162f 2023-07-30T04:06:22+02:00 Biological characteristics and permissiveness to viruses of diploid kidney cells strain from the bat Nathusius’ pipistrelle ( Pipistrellus nathusii Keyserling & Blasius, 1839; Chiroptera: Microchiroptera: Vespertilionidae ) O. S. Povolyaeva S G. Yurkov O. G. Lapteva O. L. Kolbasova A. A. Chadaeva А. Yu. Kol'tsov I. P. Sindryakova M. E. Vlasov S. P. Zhivoderov A. V. Lunitsin 2021-03-01T00:00:00Z https://doi.org/10.36233/0507-4088-12 https://doaj.org/article/c816e53d76234ec59d6ebbcf2a96162f EN RU eng rus Central Research Institute for Epidemiology https://virusjour.crie.ru/jour/article/viewFile/474/305 https://doaj.org/toc/0507-4088 https://doaj.org/toc/2411-2097 0507-4088 2411-2097 doi:10.36233/0507-4088-12 https://doaj.org/article/c816e53d76234ec59d6ebbcf2a96162f Вопросы вирусологии, Vol 66, Iss 1, Pp 29-39 (2021) bat diploid cell strain virus highly dangerous infectious diseases Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.36233/0507-4088-12 2023-07-16T00:36:10Z Introduction. Bats are an epidemiologically important natural reservoir of viruses of various taxonomic groups, including causative agents of especially dangerous infections of humans and animals. Considering the relevance of arbovirus infections, it seems advisable to study the spectrum of the sensitivity of cells derived from bats inhabiting and migrating on the territory of the Russian Federation to causative agents of vector-borne diseases of animals. The study aimed to obtain a diploid strain of cells from renal tissue of bats Pipistrellus nathusii and to investigate its biological characteristics, as well as to assess its permissiveness for bluetongue (BTV); Rift Valley fever (RVFV); lumpy skin disease (LSDV); rabbit myxoma (Myxomatosis cuniculi); rabbit, or Shope fibroma (RFV); African horse sickness (AHSV) and African swine fever (ASFV) viruses. Material and methods. There were 2 clinically healthy male individuals of P. nathusii who taken as donors of organs. To obtain diploid kidney cell culture strain and to study its properties, the level of the 6th passage was investigated by conventional cytological, virological, and molecular methods. The permissiveness of the obtained cell culture for BTV, RVFV, LSDV, Myxomatosis cuniculi, RFV, AHSV and ASFV was determined. Results. The formation of a confluent monolayer was observed after 72 hours, while the proliferation index was 2.7-3.3. The cell monolayer had been maintained without changing the medium for 45 days (observation period). The stability of the karyotype had been demonstrated in continuous subculturing at the 36th passage. The cell culture named «Diploid cell line Pipistrellus nathusii kidney», and its permissiveness to BTV, RVFV, LSDV and Myxomatosis cuniculi had been demonstrated. Discussion. The sensitivity of the strain to BTV and RVFV is consistent with the data on the identification of reovirus and RVFV in Egyptian fruit bats (Rousettus aegyptiacus), and its permissiveness for LSDV and rabbits myxoma virus is consistent with the results of ... Article in Journal/Newspaper Pipistrellus nathusii Directory of Open Access Journals: DOAJ Articles Problems of Virology 66 1 29 39 |
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Directory of Open Access Journals: DOAJ Articles |
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English Russian |
topic |
bat diploid cell strain virus highly dangerous infectious diseases Microbiology QR1-502 |
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bat diploid cell strain virus highly dangerous infectious diseases Microbiology QR1-502 O. S. Povolyaeva S G. Yurkov O. G. Lapteva O. L. Kolbasova A. A. Chadaeva А. Yu. Kol'tsov I. P. Sindryakova M. E. Vlasov S. P. Zhivoderov A. V. Lunitsin Biological characteristics and permissiveness to viruses of diploid kidney cells strain from the bat Nathusius’ pipistrelle ( Pipistrellus nathusii Keyserling & Blasius, 1839; Chiroptera: Microchiroptera: Vespertilionidae ) |
topic_facet |
bat diploid cell strain virus highly dangerous infectious diseases Microbiology QR1-502 |
description |
Introduction. Bats are an epidemiologically important natural reservoir of viruses of various taxonomic groups, including causative agents of especially dangerous infections of humans and animals. Considering the relevance of arbovirus infections, it seems advisable to study the spectrum of the sensitivity of cells derived from bats inhabiting and migrating on the territory of the Russian Federation to causative agents of vector-borne diseases of animals. The study aimed to obtain a diploid strain of cells from renal tissue of bats Pipistrellus nathusii and to investigate its biological characteristics, as well as to assess its permissiveness for bluetongue (BTV); Rift Valley fever (RVFV); lumpy skin disease (LSDV); rabbit myxoma (Myxomatosis cuniculi); rabbit, or Shope fibroma (RFV); African horse sickness (AHSV) and African swine fever (ASFV) viruses. Material and methods. There were 2 clinically healthy male individuals of P. nathusii who taken as donors of organs. To obtain diploid kidney cell culture strain and to study its properties, the level of the 6th passage was investigated by conventional cytological, virological, and molecular methods. The permissiveness of the obtained cell culture for BTV, RVFV, LSDV, Myxomatosis cuniculi, RFV, AHSV and ASFV was determined. Results. The formation of a confluent monolayer was observed after 72 hours, while the proliferation index was 2.7-3.3. The cell monolayer had been maintained without changing the medium for 45 days (observation period). The stability of the karyotype had been demonstrated in continuous subculturing at the 36th passage. The cell culture named «Diploid cell line Pipistrellus nathusii kidney», and its permissiveness to BTV, RVFV, LSDV and Myxomatosis cuniculi had been demonstrated. Discussion. The sensitivity of the strain to BTV and RVFV is consistent with the data on the identification of reovirus and RVFV in Egyptian fruit bats (Rousettus aegyptiacus), and its permissiveness for LSDV and rabbits myxoma virus is consistent with the results of ... |
format |
Article in Journal/Newspaper |
author |
O. S. Povolyaeva S G. Yurkov O. G. Lapteva O. L. Kolbasova A. A. Chadaeva А. Yu. Kol'tsov I. P. Sindryakova M. E. Vlasov S. P. Zhivoderov A. V. Lunitsin |
author_facet |
O. S. Povolyaeva S G. Yurkov O. G. Lapteva O. L. Kolbasova A. A. Chadaeva А. Yu. Kol'tsov I. P. Sindryakova M. E. Vlasov S. P. Zhivoderov A. V. Lunitsin |
author_sort |
O. S. Povolyaeva |
title |
Biological characteristics and permissiveness to viruses of diploid kidney cells strain from the bat Nathusius’ pipistrelle ( Pipistrellus nathusii Keyserling & Blasius, 1839; Chiroptera: Microchiroptera: Vespertilionidae ) |
title_short |
Biological characteristics and permissiveness to viruses of diploid kidney cells strain from the bat Nathusius’ pipistrelle ( Pipistrellus nathusii Keyserling & Blasius, 1839; Chiroptera: Microchiroptera: Vespertilionidae ) |
title_full |
Biological characteristics and permissiveness to viruses of diploid kidney cells strain from the bat Nathusius’ pipistrelle ( Pipistrellus nathusii Keyserling & Blasius, 1839; Chiroptera: Microchiroptera: Vespertilionidae ) |
title_fullStr |
Biological characteristics and permissiveness to viruses of diploid kidney cells strain from the bat Nathusius’ pipistrelle ( Pipistrellus nathusii Keyserling & Blasius, 1839; Chiroptera: Microchiroptera: Vespertilionidae ) |
title_full_unstemmed |
Biological characteristics and permissiveness to viruses of diploid kidney cells strain from the bat Nathusius’ pipistrelle ( Pipistrellus nathusii Keyserling & Blasius, 1839; Chiroptera: Microchiroptera: Vespertilionidae ) |
title_sort |
biological characteristics and permissiveness to viruses of diploid kidney cells strain from the bat nathusius’ pipistrelle ( pipistrellus nathusii keyserling & blasius, 1839; chiroptera: microchiroptera: vespertilionidae ) |
publisher |
Central Research Institute for Epidemiology |
publishDate |
2021 |
url |
https://doi.org/10.36233/0507-4088-12 https://doaj.org/article/c816e53d76234ec59d6ebbcf2a96162f |
genre |
Pipistrellus nathusii |
genre_facet |
Pipistrellus nathusii |
op_source |
Вопросы вирусологии, Vol 66, Iss 1, Pp 29-39 (2021) |
op_relation |
https://virusjour.crie.ru/jour/article/viewFile/474/305 https://doaj.org/toc/0507-4088 https://doaj.org/toc/2411-2097 0507-4088 2411-2097 doi:10.36233/0507-4088-12 https://doaj.org/article/c816e53d76234ec59d6ebbcf2a96162f |
op_doi |
https://doi.org/10.36233/0507-4088-12 |
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Problems of Virology |
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