"Candidatus Campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in Peru.
A working hypothesis is that less common species of Campylobacter (other than C. jejuni and C. coli) play a role in enteric disease among children in low resource settings and explain the gap between the detection of Campylobacter using culture and culture independent methods. "Candidatus Campy...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2022
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0010869 https://doaj.org/article/c7a1e33fcc2548cd9a3b38a5bfcc06e4 |
id |
ftdoajarticles:oai:doaj.org/article:c7a1e33fcc2548cd9a3b38a5bfcc06e4 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:c7a1e33fcc2548cd9a3b38a5bfcc06e4 2023-05-15T15:12:35+02:00 "Candidatus Campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in Peru. Paul F Garcia Bardales Francesca Schiaffino Steven Huynh Maribel Paredes Olortegui Pablo Peñataro Yori Tackeshy Pinedo Vasquez Katia Manzanares Villanueva Greisi E Curico Huansi Wagner V Shapiama Lopez Kerry K Cooper Craig T Parker Margaret N Kosek 2022-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010869 https://doaj.org/article/c7a1e33fcc2548cd9a3b38a5bfcc06e4 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010869 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010869 https://doaj.org/article/c7a1e33fcc2548cd9a3b38a5bfcc06e4 PLoS Neglected Tropical Diseases, Vol 16, Iss 10, p e0010869 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010869 2023-03-05T01:35:28Z A working hypothesis is that less common species of Campylobacter (other than C. jejuni and C. coli) play a role in enteric disease among children in low resource settings and explain the gap between the detection of Campylobacter using culture and culture independent methods. "Candidatus Campylobacter infans" (C. infans), was recently detected in stool samples from children and hypothesized to play a role in Campylobacter epidemiology in low- and middle-income countries (LMIC). This study determined the prevalence of C. infans in symptomatic and asymptomatic stool samples from children living in Iquitos, Peru. Stool samples from 215 children with diarrhea and 50 stool samples from children without diarrhea under the age of two were evaluated using a multiplex qPCR assay to detect Campylobacter spp. (16S rRNA), Campylobacter jejuni / Campylobacter coli (cadF gene), C. infans (lpxA), and Shigella spp. (ipaH). C. infans was detected in 7.9% (17/215) symptomatic samples and 4.0% (2/50) asymptomatic samples. The association between diarrhea and the presence of these targets was evaluated using univariate logistic regressions. C. infans was not associated with diarrhea. Fifty-one percent (75/146) of Campylobacter positive fecal samples were negative for C. jejuni, C. coli, and C. infans via qPCR. Shotgun metagenomics confirmed the presence of C. infans among 13 out of 14 positive C. infans positive stool samples. C infans explained only 20.7% of the diagnostic gap in stools from children with diarrhea and 16.7% of the gap in children without diarrhea. We posit that poor cadF primer performance better explains the observed gap than the prevalence of atypical non-C. jejuni/coli species. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 10 e0010869 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Paul F Garcia Bardales Francesca Schiaffino Steven Huynh Maribel Paredes Olortegui Pablo Peñataro Yori Tackeshy Pinedo Vasquez Katia Manzanares Villanueva Greisi E Curico Huansi Wagner V Shapiama Lopez Kerry K Cooper Craig T Parker Margaret N Kosek "Candidatus Campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in Peru. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
A working hypothesis is that less common species of Campylobacter (other than C. jejuni and C. coli) play a role in enteric disease among children in low resource settings and explain the gap between the detection of Campylobacter using culture and culture independent methods. "Candidatus Campylobacter infans" (C. infans), was recently detected in stool samples from children and hypothesized to play a role in Campylobacter epidemiology in low- and middle-income countries (LMIC). This study determined the prevalence of C. infans in symptomatic and asymptomatic stool samples from children living in Iquitos, Peru. Stool samples from 215 children with diarrhea and 50 stool samples from children without diarrhea under the age of two were evaluated using a multiplex qPCR assay to detect Campylobacter spp. (16S rRNA), Campylobacter jejuni / Campylobacter coli (cadF gene), C. infans (lpxA), and Shigella spp. (ipaH). C. infans was detected in 7.9% (17/215) symptomatic samples and 4.0% (2/50) asymptomatic samples. The association between diarrhea and the presence of these targets was evaluated using univariate logistic regressions. C. infans was not associated with diarrhea. Fifty-one percent (75/146) of Campylobacter positive fecal samples were negative for C. jejuni, C. coli, and C. infans via qPCR. Shotgun metagenomics confirmed the presence of C. infans among 13 out of 14 positive C. infans positive stool samples. C infans explained only 20.7% of the diagnostic gap in stools from children with diarrhea and 16.7% of the gap in children without diarrhea. We posit that poor cadF primer performance better explains the observed gap than the prevalence of atypical non-C. jejuni/coli species. |
format |
Article in Journal/Newspaper |
author |
Paul F Garcia Bardales Francesca Schiaffino Steven Huynh Maribel Paredes Olortegui Pablo Peñataro Yori Tackeshy Pinedo Vasquez Katia Manzanares Villanueva Greisi E Curico Huansi Wagner V Shapiama Lopez Kerry K Cooper Craig T Parker Margaret N Kosek |
author_facet |
Paul F Garcia Bardales Francesca Schiaffino Steven Huynh Maribel Paredes Olortegui Pablo Peñataro Yori Tackeshy Pinedo Vasquez Katia Manzanares Villanueva Greisi E Curico Huansi Wagner V Shapiama Lopez Kerry K Cooper Craig T Parker Margaret N Kosek |
author_sort |
Paul F Garcia Bardales |
title |
"Candidatus Campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in Peru. |
title_short |
"Candidatus Campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in Peru. |
title_full |
"Candidatus Campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in Peru. |
title_fullStr |
"Candidatus Campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in Peru. |
title_full_unstemmed |
"Candidatus Campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in Peru. |
title_sort |
"candidatus campylobacter infans" detection is not associated with diarrhea in children under the age of 2 in peru. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010869 https://doaj.org/article/c7a1e33fcc2548cd9a3b38a5bfcc06e4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 10, p e0010869 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010869 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010869 https://doaj.org/article/c7a1e33fcc2548cd9a3b38a5bfcc06e4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010869 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
10 |
container_start_page |
e0010869 |
_version_ |
1766343243130208256 |