Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran

Gamma Coronaviruses (GCoVs) are distributed worldwide, affecting a wide range of bird species, the beluga whale, and bottlenose dolphins. Because of the limited proofreading capability in the viral encoded polymerase, they emerge genetically diverse. There has been no molecular surveillance data to...

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Main Authors: Hannaneh Yaghoubi, A. Ghalyanchilangeroudi, Vahid Karimi, S. A. Ghafouri, M. Hashemzadeh, H. Hosseini, M. H. Fallah, F. Sadat Mousavi, H. Najafi
Format: Article in Journal/Newspaper
Language:English
French
Published: Razi Vaccine and Serum Research Institute 2019
Subjects:
Online Access:https://doi.org/10.22092/ari.2018.116786.1176
https://doaj.org/article/c79f2a2664dd4843a1d859144cb085ce
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spelling ftdoajarticles:oai:doaj.org/article:c79f2a2664dd4843a1d859144cb085ce 2023-05-15T15:41:52+02:00 Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran Hannaneh Yaghoubi A. Ghalyanchilangeroudi Vahid Karimi S. A. Ghafouri M. Hashemzadeh H. Hosseini M. H. Fallah F. Sadat Mousavi H. Najafi 2019-12-01T00:00:00Z https://doi.org/10.22092/ari.2018.116786.1176 https://doaj.org/article/c79f2a2664dd4843a1d859144cb085ce EN FR eng fre Razi Vaccine and Serum Research Institute https://archrazi.areeo.ac.ir/article_120191_c6fae8a431f025b13db442d5fbc00516.pdf https://doaj.org/toc/0365-3439 https://doaj.org/toc/2008-9872 0365-3439 2008-9872 doi:10.22092/ari.2018.116786.1176 https://doaj.org/article/c79f2a2664dd4843a1d859144cb085ce Archives of Razi Institute, Vol 74, Iss 4, Pp 349-355 (2019) gamma coronavirus molecular detection bird parks iran phylogenetic analysis Veterinary medicine SF600-1100 article 2019 ftdoajarticles https://doi.org/10.22092/ari.2018.116786.1176 2022-12-31T00:53:25Z Gamma Coronaviruses (GCoVs) are distributed worldwide, affecting a wide range of bird species, the beluga whale, and bottlenose dolphins. Because of the limited proofreading capability in the viral encoded polymerase, they emerge genetically diverse. There has been no molecular surveillance data to describe the epidemiology of GCOVs in avian species. The present study was conducted to detect GCOVs in Tehran birds’ parks, 2015. Cloacal swabs (267 samples) from eight different bird species ((Chickens ( Gallus gallus ), Pheasant ( Phasianus colchicus ), Turkey ( Meleagris gallopavo ), Partridge ( Perdix perdix ), Quail ( Coturnix coturnix ), Duck (Anas platyrhynchos), Goose (Anserini),and Guinea fowl (Numididae)) were collected, the viral RNA was extracted, the RT-PCR was performed using QIAGEN one step RT-PCR kit and the primers targeting “3'-UTR” and “Nucleocapsid” genes. The detection rate was approximately 8.99%. GCOVs were detected in the chicken, quail, pheasant, turkey, and the partridge with different prevalence rates. Phylogenetic tree based on partial nucleotide sequences of the N gene clustered the samples into two groups. It is the first report of GCOVs in non-commercial birds in Iran. According to our results, GCOVs are circulating in different avian species, and further studies are needed to isolate these viruses and evaluate their pathogenesis. Article in Journal/Newspaper Beluga Beluga whale Beluga* Directory of Open Access Journals: DOAJ Articles
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
French
topic gamma coronavirus
molecular detection
bird parks
iran
phylogenetic analysis
Veterinary medicine
SF600-1100
spellingShingle gamma coronavirus
molecular detection
bird parks
iran
phylogenetic analysis
Veterinary medicine
SF600-1100
Hannaneh Yaghoubi
A. Ghalyanchilangeroudi
Vahid Karimi
S. A. Ghafouri
M. Hashemzadeh
H. Hosseini
M. H. Fallah
F. Sadat Mousavi
H. Najafi
Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran
topic_facet gamma coronavirus
molecular detection
bird parks
iran
phylogenetic analysis
Veterinary medicine
SF600-1100
description Gamma Coronaviruses (GCoVs) are distributed worldwide, affecting a wide range of bird species, the beluga whale, and bottlenose dolphins. Because of the limited proofreading capability in the viral encoded polymerase, they emerge genetically diverse. There has been no molecular surveillance data to describe the epidemiology of GCOVs in avian species. The present study was conducted to detect GCOVs in Tehran birds’ parks, 2015. Cloacal swabs (267 samples) from eight different bird species ((Chickens ( Gallus gallus ), Pheasant ( Phasianus colchicus ), Turkey ( Meleagris gallopavo ), Partridge ( Perdix perdix ), Quail ( Coturnix coturnix ), Duck (Anas platyrhynchos), Goose (Anserini),and Guinea fowl (Numididae)) were collected, the viral RNA was extracted, the RT-PCR was performed using QIAGEN one step RT-PCR kit and the primers targeting “3'-UTR” and “Nucleocapsid” genes. The detection rate was approximately 8.99%. GCOVs were detected in the chicken, quail, pheasant, turkey, and the partridge with different prevalence rates. Phylogenetic tree based on partial nucleotide sequences of the N gene clustered the samples into two groups. It is the first report of GCOVs in non-commercial birds in Iran. According to our results, GCOVs are circulating in different avian species, and further studies are needed to isolate these viruses and evaluate their pathogenesis.
format Article in Journal/Newspaper
author Hannaneh Yaghoubi
A. Ghalyanchilangeroudi
Vahid Karimi
S. A. Ghafouri
M. Hashemzadeh
H. Hosseini
M. H. Fallah
F. Sadat Mousavi
H. Najafi
author_facet Hannaneh Yaghoubi
A. Ghalyanchilangeroudi
Vahid Karimi
S. A. Ghafouri
M. Hashemzadeh
H. Hosseini
M. H. Fallah
F. Sadat Mousavi
H. Najafi
author_sort Hannaneh Yaghoubi
title Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran
title_short Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran
title_full Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran
title_fullStr Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran
title_full_unstemmed Molecular Detection of Gamma Coronaviruses in Bird Parks of Iran
title_sort molecular detection of gamma coronaviruses in bird parks of iran
publisher Razi Vaccine and Serum Research Institute
publishDate 2019
url https://doi.org/10.22092/ari.2018.116786.1176
https://doaj.org/article/c79f2a2664dd4843a1d859144cb085ce
genre Beluga
Beluga whale
Beluga*
genre_facet Beluga
Beluga whale
Beluga*
op_source Archives of Razi Institute, Vol 74, Iss 4, Pp 349-355 (2019)
op_relation https://archrazi.areeo.ac.ir/article_120191_c6fae8a431f025b13db442d5fbc00516.pdf
https://doaj.org/toc/0365-3439
https://doaj.org/toc/2008-9872
0365-3439
2008-9872
doi:10.22092/ari.2018.116786.1176
https://doaj.org/article/c79f2a2664dd4843a1d859144cb085ce
op_doi https://doi.org/10.22092/ari.2018.116786.1176
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