Partial purification of peptides present in the Tityus macrochirus (Buthidae) scorpion venom and preliminary assessment of their cytotoxicity
Introduction: Scorpion venom contains peptides with neurotoxic action primarily active on ion channels in the nervous system of insects and mammals. They are also characterized as cytolytic and anticancer, biological characteristics that have not yet been reported for the Tityus macrochirus venom. O...
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ftdoajarticles:oai:doaj.org/article:c784669f9ac441f6a87d14d75be6c8b1 2023-05-15T15:10:02+02:00 Partial purification of peptides present in the Tityus macrochirus (Buthidae) scorpion venom and preliminary assessment of their cytotoxicity Clara Andrea Rincón-Cortés Edgar Antonio Reyes-Montaño Nohora Angélica Vega-Castro 2017-06-01T00:00:00Z https://doi.org/10.7705/biomedica.v37i3.3265 https://doaj.org/article/c784669f9ac441f6a87d14d75be6c8b1 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3265 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i3.3265 https://doaj.org/article/c784669f9ac441f6a87d14d75be6c8b1 Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 2, Pp 238-249 (2017) Scorpion venoms peptides/isolation & purification neoplasms Medicine R Arctic medicine. Tropical medicine RC955-962 article 2017 ftdoajarticles https://doi.org/10.7705/biomedica.v37i3.3265 2022-12-30T23:44:44Z Introduction: Scorpion venom contains peptides with neurotoxic action primarily active on ion channels in the nervous system of insects and mammals. They are also characterized as cytolytic and anticancer, biological characteristics that have not yet been reported for the Tityus macrochirus venom. Objective: To assess if the total T. macrochirus venom and the fraction of partially purified peptides decrease the viability of various tumor-derived cell lines. Materials and methods: The scorpion venom was collected by electrical stimulation and, subsequently, subjected to chromatography, electrophoresis, and ultrafiltration with Amicon Ultra 0.5® membranes for the partial identification and purification of its peptides. The cytotoxic activity of the venom and the peptides fraction trials on tumor-derived cell lines were carried out by the MTT method. Results: The T. macrochirus scorpion venom has peptides with molecular weights ranging between 3 and 10 kDa. They were partially purified using the ultrafiltration technique, and assessed by the RP-HPLC method. Cytotoxicity trials with the whole T. macrochirus venom showed a higher viability decrease on the PC3 cell line compared to the other cell lines assessed, while the partially purified peptides decreased the HeLa cell line viability. Conclusion: Peptides in the T. macrochirus scorpion venom showed cytotoxic activity on some tumorderived cell lines. We observed some degree of selectivity against other cell lines assessed. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 37 2 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English Spanish |
topic |
Scorpion venoms peptides/isolation & purification neoplasms Medicine R Arctic medicine. Tropical medicine RC955-962 |
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Scorpion venoms peptides/isolation & purification neoplasms Medicine R Arctic medicine. Tropical medicine RC955-962 Clara Andrea Rincón-Cortés Edgar Antonio Reyes-Montaño Nohora Angélica Vega-Castro Partial purification of peptides present in the Tityus macrochirus (Buthidae) scorpion venom and preliminary assessment of their cytotoxicity |
topic_facet |
Scorpion venoms peptides/isolation & purification neoplasms Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Scorpion venom contains peptides with neurotoxic action primarily active on ion channels in the nervous system of insects and mammals. They are also characterized as cytolytic and anticancer, biological characteristics that have not yet been reported for the Tityus macrochirus venom. Objective: To assess if the total T. macrochirus venom and the fraction of partially purified peptides decrease the viability of various tumor-derived cell lines. Materials and methods: The scorpion venom was collected by electrical stimulation and, subsequently, subjected to chromatography, electrophoresis, and ultrafiltration with Amicon Ultra 0.5® membranes for the partial identification and purification of its peptides. The cytotoxic activity of the venom and the peptides fraction trials on tumor-derived cell lines were carried out by the MTT method. Results: The T. macrochirus scorpion venom has peptides with molecular weights ranging between 3 and 10 kDa. They were partially purified using the ultrafiltration technique, and assessed by the RP-HPLC method. Cytotoxicity trials with the whole T. macrochirus venom showed a higher viability decrease on the PC3 cell line compared to the other cell lines assessed, while the partially purified peptides decreased the HeLa cell line viability. Conclusion: Peptides in the T. macrochirus scorpion venom showed cytotoxic activity on some tumorderived cell lines. We observed some degree of selectivity against other cell lines assessed. |
format |
Article in Journal/Newspaper |
author |
Clara Andrea Rincón-Cortés Edgar Antonio Reyes-Montaño Nohora Angélica Vega-Castro |
author_facet |
Clara Andrea Rincón-Cortés Edgar Antonio Reyes-Montaño Nohora Angélica Vega-Castro |
author_sort |
Clara Andrea Rincón-Cortés |
title |
Partial purification of peptides present in the Tityus macrochirus (Buthidae) scorpion venom and preliminary assessment of their cytotoxicity |
title_short |
Partial purification of peptides present in the Tityus macrochirus (Buthidae) scorpion venom and preliminary assessment of their cytotoxicity |
title_full |
Partial purification of peptides present in the Tityus macrochirus (Buthidae) scorpion venom and preliminary assessment of their cytotoxicity |
title_fullStr |
Partial purification of peptides present in the Tityus macrochirus (Buthidae) scorpion venom and preliminary assessment of their cytotoxicity |
title_full_unstemmed |
Partial purification of peptides present in the Tityus macrochirus (Buthidae) scorpion venom and preliminary assessment of their cytotoxicity |
title_sort |
partial purification of peptides present in the tityus macrochirus (buthidae) scorpion venom and preliminary assessment of their cytotoxicity |
publisher |
Instituto Nacional de Salud |
publishDate |
2017 |
url |
https://doi.org/10.7705/biomedica.v37i3.3265 https://doaj.org/article/c784669f9ac441f6a87d14d75be6c8b1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 2, Pp 238-249 (2017) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3265 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i3.3265 https://doaj.org/article/c784669f9ac441f6a87d14d75be6c8b1 |
op_doi |
https://doi.org/10.7705/biomedica.v37i3.3265 |
container_title |
Biomédica |
container_volume |
37 |
container_issue |
2 |
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1766341104631808000 |