Validation study of HLA-B*13:01 as a biomarker of dapsone hypersensitivity syndrome in leprosy patients in Indonesia.
Leprosy is a stigmatizing, chronic infection which degenerates the nervous system and often leads to incapacitation. Multi-drug therapy which consists of dapsone, rifampicin and clofazimine has been effective to combat this disease. In Indonesia, especially in Papua Island, leprosy is still a proble...
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ftdoajarticles:oai:doaj.org/article:c783291113b74b05aa9da407139ec057 2023-05-15T15:13:27+02:00 Validation study of HLA-B*13:01 as a biomarker of dapsone hypersensitivity syndrome in leprosy patients in Indonesia. Hana Krismawati Astrid Irwanto Arry Pongtiku Ishak Darryl Irwan Yustinus Maladan Yuli Arisanti Sitanggang Tri Wahyuni Ratna Tanjung Yonghu Sun Hong Liu Furen Zhang Antonius Oktavian Jianjun Liu 2020-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008746 https://doaj.org/article/c783291113b74b05aa9da407139ec057 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008746 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008746 https://doaj.org/article/c783291113b74b05aa9da407139ec057 PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008746 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008746 2022-12-31T07:48:33Z Leprosy is a stigmatizing, chronic infection which degenerates the nervous system and often leads to incapacitation. Multi-drug therapy which consists of dapsone, rifampicin and clofazimine has been effective to combat this disease. In Indonesia, especially in Papua Island, leprosy is still a problem. Furthermore, there had been higher reports of Dapsone Hypersensitivity Syndrome (DHS) which also challenges leprosy elimination in certain aspects. Globally, DHS has a prevalence rate of 1.4% and a fatality rate up to 13%. The aim of this study is to validate HLA-B*13:01, a previously discovered biomarker for DHS in the Chinese population, as a biomarker for DHS in the Papua population.This is a case-control study of 34 leprosy patients who presented themselves with DHS (case subjects) and 52 leprosy patients without DHS (control subjects). Patients were recruited from 2 provinces: Papua and West Papua. DNA was extracted from 3 ml blood specimens. HLA-B alleles were typed using the gold-standard sequence based typing method. Results were then analysed using logistic regression and risk assessment was carried out. The results of HLA-typing showed that HLA-B*13:01 was the most significant allele associated with DHS, with odds ratio = 233.64 and P-value = 7.11×10-9, confirming the strong association of HLA-B*13:01 to DHS in the Papua population. The sensitivity of this biomarker is 91.2% and specificity is 96.2%, with an area under the curve of 0.95. HLA-B*13:01 is validated as a biomarker for DHS in leprosy patients in Papua, Indonesia, and can potentially be a good predictor of DHS to help prevent this condition in the future. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Papua Island ENVELOPE(-55.971,-55.971,-63.129,-63.129) PLOS Neglected Tropical Diseases 14 10 e0008746 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hana Krismawati Astrid Irwanto Arry Pongtiku Ishak Darryl Irwan Yustinus Maladan Yuli Arisanti Sitanggang Tri Wahyuni Ratna Tanjung Yonghu Sun Hong Liu Furen Zhang Antonius Oktavian Jianjun Liu Validation study of HLA-B*13:01 as a biomarker of dapsone hypersensitivity syndrome in leprosy patients in Indonesia. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leprosy is a stigmatizing, chronic infection which degenerates the nervous system and often leads to incapacitation. Multi-drug therapy which consists of dapsone, rifampicin and clofazimine has been effective to combat this disease. In Indonesia, especially in Papua Island, leprosy is still a problem. Furthermore, there had been higher reports of Dapsone Hypersensitivity Syndrome (DHS) which also challenges leprosy elimination in certain aspects. Globally, DHS has a prevalence rate of 1.4% and a fatality rate up to 13%. The aim of this study is to validate HLA-B*13:01, a previously discovered biomarker for DHS in the Chinese population, as a biomarker for DHS in the Papua population.This is a case-control study of 34 leprosy patients who presented themselves with DHS (case subjects) and 52 leprosy patients without DHS (control subjects). Patients were recruited from 2 provinces: Papua and West Papua. DNA was extracted from 3 ml blood specimens. HLA-B alleles were typed using the gold-standard sequence based typing method. Results were then analysed using logistic regression and risk assessment was carried out. The results of HLA-typing showed that HLA-B*13:01 was the most significant allele associated with DHS, with odds ratio = 233.64 and P-value = 7.11×10-9, confirming the strong association of HLA-B*13:01 to DHS in the Papua population. The sensitivity of this biomarker is 91.2% and specificity is 96.2%, with an area under the curve of 0.95. HLA-B*13:01 is validated as a biomarker for DHS in leprosy patients in Papua, Indonesia, and can potentially be a good predictor of DHS to help prevent this condition in the future. |
format |
Article in Journal/Newspaper |
author |
Hana Krismawati Astrid Irwanto Arry Pongtiku Ishak Darryl Irwan Yustinus Maladan Yuli Arisanti Sitanggang Tri Wahyuni Ratna Tanjung Yonghu Sun Hong Liu Furen Zhang Antonius Oktavian Jianjun Liu |
author_facet |
Hana Krismawati Astrid Irwanto Arry Pongtiku Ishak Darryl Irwan Yustinus Maladan Yuli Arisanti Sitanggang Tri Wahyuni Ratna Tanjung Yonghu Sun Hong Liu Furen Zhang Antonius Oktavian Jianjun Liu |
author_sort |
Hana Krismawati |
title |
Validation study of HLA-B*13:01 as a biomarker of dapsone hypersensitivity syndrome in leprosy patients in Indonesia. |
title_short |
Validation study of HLA-B*13:01 as a biomarker of dapsone hypersensitivity syndrome in leprosy patients in Indonesia. |
title_full |
Validation study of HLA-B*13:01 as a biomarker of dapsone hypersensitivity syndrome in leprosy patients in Indonesia. |
title_fullStr |
Validation study of HLA-B*13:01 as a biomarker of dapsone hypersensitivity syndrome in leprosy patients in Indonesia. |
title_full_unstemmed |
Validation study of HLA-B*13:01 as a biomarker of dapsone hypersensitivity syndrome in leprosy patients in Indonesia. |
title_sort |
validation study of hla-b*13:01 as a biomarker of dapsone hypersensitivity syndrome in leprosy patients in indonesia. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008746 https://doaj.org/article/c783291113b74b05aa9da407139ec057 |
long_lat |
ENVELOPE(-55.971,-55.971,-63.129,-63.129) |
geographic |
Arctic Papua Island |
geographic_facet |
Arctic Papua Island |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 10, p e0008746 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008746 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008746 https://doaj.org/article/c783291113b74b05aa9da407139ec057 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008746 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
10 |
container_start_page |
e0008746 |
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1766343997198958592 |