Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii
ABSTRACT Toxoplasma gondii can cross the placental barrier, causing fetal infection with potentially severe sequelae. The aim of this study was to evaluate whether the serological screening for toxoplasmosis should be included in the basic neonatal heel prick test in order to establish criteria for...
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ftdoajarticles:oai:doaj.org/article:c774cf2f9ecc48de80c5dc820ca882cc 2024-09-09T19:27:12+00:00 Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii Heloisa Ribeiro Storchilo Hanstter Hallison Alves Rezende Taynara Cristina Gomes Jéssica Yonara de Souza Antonio Roberto Gomes Junior Mariza Martins Avelino Waldemar Naves do Amaral Ana Maria de Castro https://doi.org/10.1590/s1678-9946201961030 https://doaj.org/article/c774cf2f9ecc48de80c5dc820ca882cc EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652019005000214&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201961030 https://doaj.org/article/c774cf2f9ecc48de80c5dc820ca882cc Revista do Instituto de Medicina Tropical de São Paulo, Vol 61 Serological tests Neonatal screening Newborn Congenital toxoplasmosis Heel prick test Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article ftdoajarticles https://doi.org/10.1590/s1678-9946201961030 2024-08-05T17:49:30Z ABSTRACT Toxoplasma gondii can cross the placental barrier, causing fetal infection with potentially severe sequelae. The aim of this study was to evaluate whether the serological screening for toxoplasmosis should be included in the basic neonatal heel prick test in order to establish criteria for the confirmation and/or exclusion of the diagnosis of congenital infection in newborns treated at three public health units in the metropolitan region of Goiania, Goias State, Brazil. Blood samples were collected on filter paper from newborns and later, peripheral blood samples from the mothers and their respective children were obtained to confirm or exclude the diagnosis of suspected congenital infection, by means of an enzyme-linked immunosorbent assay (IgM and IgG) and a polymerase chain reaction assay. From a total of 1,159 blood samples collected on filter paper, 43.92% were reactive to IgG and 0.17% to anti-T. gondii IgM and IgG. One hundred and twenty-seven paired samples (mother and child) were collected following consensual protocols for peripheral blood collection. Results obtained from the filter paper and peripheral blood of the newborns were 90.55% concordant. A comparison of the mother and child blood test results showed agreement regarding the detection of IgG in 90.48% of the samples. The parasite DNA was detected in the peripheral blood of one child. In view of the results obtained in this study, the inclusion of the serological screening for toxoplasmosis in the newborn heel prick test proved to be effective for the early detection of congenital T. gondii infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 61 |
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Serological tests Neonatal screening Newborn Congenital toxoplasmosis Heel prick test Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Serological tests Neonatal screening Newborn Congenital toxoplasmosis Heel prick test Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Heloisa Ribeiro Storchilo Hanstter Hallison Alves Rezende Taynara Cristina Gomes Jéssica Yonara de Souza Antonio Roberto Gomes Junior Mariza Martins Avelino Waldemar Naves do Amaral Ana Maria de Castro Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii |
topic_facet |
Serological tests Neonatal screening Newborn Congenital toxoplasmosis Heel prick test Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Toxoplasma gondii can cross the placental barrier, causing fetal infection with potentially severe sequelae. The aim of this study was to evaluate whether the serological screening for toxoplasmosis should be included in the basic neonatal heel prick test in order to establish criteria for the confirmation and/or exclusion of the diagnosis of congenital infection in newborns treated at three public health units in the metropolitan region of Goiania, Goias State, Brazil. Blood samples were collected on filter paper from newborns and later, peripheral blood samples from the mothers and their respective children were obtained to confirm or exclude the diagnosis of suspected congenital infection, by means of an enzyme-linked immunosorbent assay (IgM and IgG) and a polymerase chain reaction assay. From a total of 1,159 blood samples collected on filter paper, 43.92% were reactive to IgG and 0.17% to anti-T. gondii IgM and IgG. One hundred and twenty-seven paired samples (mother and child) were collected following consensual protocols for peripheral blood collection. Results obtained from the filter paper and peripheral blood of the newborns were 90.55% concordant. A comparison of the mother and child blood test results showed agreement regarding the detection of IgG in 90.48% of the samples. The parasite DNA was detected in the peripheral blood of one child. In view of the results obtained in this study, the inclusion of the serological screening for toxoplasmosis in the newborn heel prick test proved to be effective for the early detection of congenital T. gondii infection. |
format |
Article in Journal/Newspaper |
author |
Heloisa Ribeiro Storchilo Hanstter Hallison Alves Rezende Taynara Cristina Gomes Jéssica Yonara de Souza Antonio Roberto Gomes Junior Mariza Martins Avelino Waldemar Naves do Amaral Ana Maria de Castro |
author_facet |
Heloisa Ribeiro Storchilo Hanstter Hallison Alves Rezende Taynara Cristina Gomes Jéssica Yonara de Souza Antonio Roberto Gomes Junior Mariza Martins Avelino Waldemar Naves do Amaral Ana Maria de Castro |
author_sort |
Heloisa Ribeiro Storchilo |
title |
Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii |
title_short |
Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii |
title_full |
Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii |
title_fullStr |
Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii |
title_full_unstemmed |
Basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by Toxoplasma gondii |
title_sort |
basic heel prick test: inclusion of screening, diagnosis and criteria for early confirmation of congenital infection by toxoplasma gondii |
publisher |
Universidade de São Paulo (USP) |
url |
https://doi.org/10.1590/s1678-9946201961030 https://doaj.org/article/c774cf2f9ecc48de80c5dc820ca882cc |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 61 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652019005000214&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201961030 https://doaj.org/article/c774cf2f9ecc48de80c5dc820ca882cc |
op_doi |
https://doi.org/10.1590/s1678-9946201961030 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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61 |
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