Plasmodium falciparum isolates from Angola show the S tct VMNT haplotype in the pfcrt gene
Abstract Background Effective treatment remains a mainstay of malaria control, but it is unfortunately strongly compromised by drug resistance, particularly in Plasmodium falciparum , the most important human malaria parasite. Although P. falciparum chemoresistance is well recognized all over the wo...
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ftdoajarticles:oai:doaj.org/article:c7441632f8e84b479546fc727f04d1ae 2023-05-15T15:15:54+02:00 Plasmodium falciparum isolates from Angola show the S tct VMNT haplotype in the pfcrt gene Lutucuta Kosi Florbela JI Pereira-Carvalho Guilhermina AL Gama Bianca E Almeida de Oliveira Natália K Fortes Filomeno Rosenthal Philip J Daniel-Ribeiro Cláudio T de Fátima Ferreira-da-Cruz Maria 2010-06-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-174 https://doaj.org/article/c7441632f8e84b479546fc727f04d1ae EN eng BMC http://www.malariajournal.com/content/9/1/174 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-174 1475-2875 https://doaj.org/article/c7441632f8e84b479546fc727f04d1ae Malaria Journal, Vol 9, Iss 1, p 174 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-174 2022-12-31T08:19:24Z Abstract Background Effective treatment remains a mainstay of malaria control, but it is unfortunately strongly compromised by drug resistance, particularly in Plasmodium falciparum , the most important human malaria parasite. Although P. falciparum chemoresistance is well recognized all over the world, limited data are available on the distribution and prevalence of pfcrt and pfmdr1 haplotypes that mediate resistance to commonly used drugs and that show distinct geographic differences. Methods Plasmodium falciparum -infected blood samples collected in 2007 at four municipalities of Luanda, Angola, were genotyped using PCR and direct DNA sequencing. Single nucleotide polymorphisms in the P. falciparum pfcrt and pfmdr1 genes were assessed and haplotype prevalences were determined. Results and Discussion The most prevalent pfcrt haplotype was S tct VMN T (representing amino acids at codons 72-76). This result was unexpected, since the S tct VMN T haplotype has previously been seen mainly in parasites from South America and India. The CV IET , CVMN T and CV I N T drug-resistance haplotypes were also found, and one previously undescribed haplotype (CVM DT ) was detected. Regarding pfmdr1 , the most prevalent haplotype was Y EYSNVD (representing amino acids at codons 86, 130, 184, 1034, 1042, 1109 and 1246). Wild haplotypes for pfcrt and pfmdr1 were uncommon; 3% of field isolates harbored wild type pfcrt (CVMNK), whereas 21% had wild type pfmdr1 (NEYSNVD). The observed predominance of the S tct VMN T haplotype in Angola could be a result of frequent travel between Brazil and Angola citizens in the context of selective pressure of heavy CQ use. Conclusions The high prevalence of the pfcrt S VMN T haplotype and the pfmdr1 86 Y mutation confirm high-level chloroquine resistance and might suggest reduced efficacy of amodiaquine in Angola. Further studies must be encouraged to examine the in vitro sensitivity of pfcrt S VMN T parasites to artesunate and amodiaquine for better conclusive data. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 174 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Lutucuta Kosi Florbela JI Pereira-Carvalho Guilhermina AL Gama Bianca E Almeida de Oliveira Natália K Fortes Filomeno Rosenthal Philip J Daniel-Ribeiro Cláudio T de Fátima Ferreira-da-Cruz Maria Plasmodium falciparum isolates from Angola show the S tct VMNT haplotype in the pfcrt gene |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Effective treatment remains a mainstay of malaria control, but it is unfortunately strongly compromised by drug resistance, particularly in Plasmodium falciparum , the most important human malaria parasite. Although P. falciparum chemoresistance is well recognized all over the world, limited data are available on the distribution and prevalence of pfcrt and pfmdr1 haplotypes that mediate resistance to commonly used drugs and that show distinct geographic differences. Methods Plasmodium falciparum -infected blood samples collected in 2007 at four municipalities of Luanda, Angola, were genotyped using PCR and direct DNA sequencing. Single nucleotide polymorphisms in the P. falciparum pfcrt and pfmdr1 genes were assessed and haplotype prevalences were determined. Results and Discussion The most prevalent pfcrt haplotype was S tct VMN T (representing amino acids at codons 72-76). This result was unexpected, since the S tct VMN T haplotype has previously been seen mainly in parasites from South America and India. The CV IET , CVMN T and CV I N T drug-resistance haplotypes were also found, and one previously undescribed haplotype (CVM DT ) was detected. Regarding pfmdr1 , the most prevalent haplotype was Y EYSNVD (representing amino acids at codons 86, 130, 184, 1034, 1042, 1109 and 1246). Wild haplotypes for pfcrt and pfmdr1 were uncommon; 3% of field isolates harbored wild type pfcrt (CVMNK), whereas 21% had wild type pfmdr1 (NEYSNVD). The observed predominance of the S tct VMN T haplotype in Angola could be a result of frequent travel between Brazil and Angola citizens in the context of selective pressure of heavy CQ use. Conclusions The high prevalence of the pfcrt S VMN T haplotype and the pfmdr1 86 Y mutation confirm high-level chloroquine resistance and might suggest reduced efficacy of amodiaquine in Angola. Further studies must be encouraged to examine the in vitro sensitivity of pfcrt S VMN T parasites to artesunate and amodiaquine for better conclusive data. |
format |
Article in Journal/Newspaper |
author |
Lutucuta Kosi Florbela JI Pereira-Carvalho Guilhermina AL Gama Bianca E Almeida de Oliveira Natália K Fortes Filomeno Rosenthal Philip J Daniel-Ribeiro Cláudio T de Fátima Ferreira-da-Cruz Maria |
author_facet |
Lutucuta Kosi Florbela JI Pereira-Carvalho Guilhermina AL Gama Bianca E Almeida de Oliveira Natália K Fortes Filomeno Rosenthal Philip J Daniel-Ribeiro Cláudio T de Fátima Ferreira-da-Cruz Maria |
author_sort |
Lutucuta Kosi Florbela JI |
title |
Plasmodium falciparum isolates from Angola show the S tct VMNT haplotype in the pfcrt gene |
title_short |
Plasmodium falciparum isolates from Angola show the S tct VMNT haplotype in the pfcrt gene |
title_full |
Plasmodium falciparum isolates from Angola show the S tct VMNT haplotype in the pfcrt gene |
title_fullStr |
Plasmodium falciparum isolates from Angola show the S tct VMNT haplotype in the pfcrt gene |
title_full_unstemmed |
Plasmodium falciparum isolates from Angola show the S tct VMNT haplotype in the pfcrt gene |
title_sort |
plasmodium falciparum isolates from angola show the s tct vmnt haplotype in the pfcrt gene |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-174 https://doaj.org/article/c7441632f8e84b479546fc727f04d1ae |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 174 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/174 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-174 1475-2875 https://doaj.org/article/c7441632f8e84b479546fc727f04d1ae |
op_doi |
https://doi.org/10.1186/1475-2875-9-174 |
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Malaria Journal |
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9 |
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174 |
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1766346230969925632 |