Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages

• Published in: Fisheries and Aquatic Sciences
• Main Authors: Hong-Deok Kim, Soo-Bin Lee, Seok-Chun Ko, Won-Kyo Jung, Young-Mog Kim, Seon-Bong Kim
• Format: Article in Journal/Newspaper
• Language: English
• Published: The Korean Society of Fisheries and Aquatic Science 2018
• Subjects: Ozonated krill oil; Anti-inflammatory; RAW 264.7 macrophages; MAPKs; Aquaculture. Fisheries. Angling; SH1-691; Euphausia superba
• Online Access: https://doi.org/10.1186/s41240-018-0092-1; https://doaj.org/article/c72a441225dd42f8a67a4ee0d8f3e93e

Abstract Background Inflammation has been known to associate with many human diseases. The objective of this study was to evaluate an anti-inflammatory effect of ozonated krill (Euphausia superba) oil, which was prepared by the treatment of krill oil using ozone gas. The anti-inflammatory activity was evaluated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Results Ozonated krill oil significantly inhibited nitric oxide (NO) production and suppressed the mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 macrophages. Ozonated krill oil also reduced the mRNA expression of inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 macrophages. To elucidate the mechanism underlying the anti-inflammatory activity of ozonated krill oil, we evaluated the effects of ozonated krill oil on the activation of mitogen-activated protein kinases (MAPKs) pathway. Ozonated krill oil suppressed the LPS-stimulated phosphorylation of p38 MAPK and c-Jun N-terminal kinases (JNK). Conclusion This study revealed that the ozonated krill oil exhibited an anti-inflammatory effect in LPS-stimulated RAW 264.7 macrophages. To the best of our knowledge, this is the first report that ozonated krill oil suppressed pro-inflammatory mediator and cytokine expression in LPS-stimulated RAW 264.7 macrophages by inhibiting the phosphorylation of p38 MAPK and JNK.