Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system
Total dissolved inorganic carbon ( C T ) is one of the most frequently measured parameters used to calculate the partial pressure of carbon dioxide in seawater. Its determination has become increasingly important because of the rising interest in the biological effects of ocean acidification. Coulom...
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ftdoajarticles:oai:doaj.org/article:c727f92d44b14d939dda45572679e64b 2023-05-15T17:52:04+02:00 Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system T. Hansen B. Gardeler B. Matthiessen 2013-10-01T00:00:00Z https://doi.org/10.5194/bg-10-6601-2013 https://doaj.org/article/c727f92d44b14d939dda45572679e64b EN eng Copernicus Publications http://www.biogeosciences.net/10/6601/2013/bg-10-6601-2013.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-10-6601-2013 1726-4170 1726-4189 https://doaj.org/article/c727f92d44b14d939dda45572679e64b Biogeosciences, Vol 10, Iss 10, Pp 6601-6608 (2013) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2013 ftdoajarticles https://doi.org/10.5194/bg-10-6601-2013 2022-12-31T14:42:35Z Total dissolved inorganic carbon ( C T ) is one of the most frequently measured parameters used to calculate the partial pressure of carbon dioxide in seawater. Its determination has become increasingly important because of the rising interest in the biological effects of ocean acidification. Coulometric and infrared detection methods are currently favored in order to precisely quantify C T . These methods however are not sufficiently validated for C T measurements of biological experiments manipulating seawater carbonate chemistry with an extended C T measurement range (~1250–2400 μmol kg –1 ) compared to natural open ocean seawater (~1950–2200 μmol kg −1 ). The requirement of total sample amounts between 0.1–1 L seawater in the coulometric- and infrared detection methods potentially exclude their use for experiments working with much smaller volumes. Additionally, precise C T analytics become difficult with high amounts of biomass (e.g., phytoplankton cultures) or even impossible in the presence of planktonic calcifiers without sample pre-filtration. Filtration however, can alter C T concentration through gas exchange induced by high pressure. Addressing these problems, we present precise quantification of C T using a small, basic and inexpensive gas chromatograph as a C T analyzer. Our technique is able to provide a repeatability of ±3.1 μmol kg −1 , given by the pooled standard deviation over a C T range typically applied in acidification experiments. 200 μL of sample is required to perform the actual C T measurement. The total sample amount needed is 12 mL. Moreover, we show that sample filtration is applicable with only minor alteration of the C T . The method is simple, reliable and with low cumulative material costs. Hence, it is potentially attractive for all researchers experimentally manipulating the seawater carbonate system. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles Biogeosciences 10 10 6601 6608 |
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English |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 T. Hansen B. Gardeler B. Matthiessen Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
Total dissolved inorganic carbon ( C T ) is one of the most frequently measured parameters used to calculate the partial pressure of carbon dioxide in seawater. Its determination has become increasingly important because of the rising interest in the biological effects of ocean acidification. Coulometric and infrared detection methods are currently favored in order to precisely quantify C T . These methods however are not sufficiently validated for C T measurements of biological experiments manipulating seawater carbonate chemistry with an extended C T measurement range (~1250–2400 μmol kg –1 ) compared to natural open ocean seawater (~1950–2200 μmol kg −1 ). The requirement of total sample amounts between 0.1–1 L seawater in the coulometric- and infrared detection methods potentially exclude their use for experiments working with much smaller volumes. Additionally, precise C T analytics become difficult with high amounts of biomass (e.g., phytoplankton cultures) or even impossible in the presence of planktonic calcifiers without sample pre-filtration. Filtration however, can alter C T concentration through gas exchange induced by high pressure. Addressing these problems, we present precise quantification of C T using a small, basic and inexpensive gas chromatograph as a C T analyzer. Our technique is able to provide a repeatability of ±3.1 μmol kg −1 , given by the pooled standard deviation over a C T range typically applied in acidification experiments. 200 μL of sample is required to perform the actual C T measurement. The total sample amount needed is 12 mL. Moreover, we show that sample filtration is applicable with only minor alteration of the C T . The method is simple, reliable and with low cumulative material costs. Hence, it is potentially attractive for all researchers experimentally manipulating the seawater carbonate system. |
format |
Article in Journal/Newspaper |
author |
T. Hansen B. Gardeler B. Matthiessen |
author_facet |
T. Hansen B. Gardeler B. Matthiessen |
author_sort |
T. Hansen |
title |
Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
title_short |
Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
title_full |
Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
title_fullStr |
Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
title_full_unstemmed |
Technical Note: Precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
title_sort |
technical note: precise quantitative measurements of total dissolved inorganic carbon from small amounts of seawater using a gas chromatographic system |
publisher |
Copernicus Publications |
publishDate |
2013 |
url |
https://doi.org/10.5194/bg-10-6601-2013 https://doaj.org/article/c727f92d44b14d939dda45572679e64b |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Biogeosciences, Vol 10, Iss 10, Pp 6601-6608 (2013) |
op_relation |
http://www.biogeosciences.net/10/6601/2013/bg-10-6601-2013.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-10-6601-2013 1726-4170 1726-4189 https://doaj.org/article/c727f92d44b14d939dda45572679e64b |
op_doi |
https://doi.org/10.5194/bg-10-6601-2013 |
container_title |
Biogeosciences |
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10 |
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10 |
container_start_page |
6601 |
op_container_end_page |
6608 |
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1766159390692343808 |