The Distribution of IgT mRNA + Cells in the Gut of the Atlantic Salmon ( Salmo salar L.)

The newly discovered IgT + B cell is thought to play a dominant role in mucosal immunity, but limited studies have examined its distribution in fish species, hindering our understanding of its function. This study investigated IgT and poly Ig receptor (pIgR) mRNA + cell distribution in Atlantic salm...

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Bibliographic Details
Published in:Animals
Main Authors: Pedro Luis Castro, Fran Barac, Tom Johnny Hansen, Per Gunnar Fjelldal, Ivar Hordvik, Håvard Bjørgen, Erling Olaf Koppang
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2023
Subjects:
Atlantic salmon
gut
IgT
pIgR
in situ hybridization
mucosal immunity
Veterinary medicine
SF600-1100
Zoology
QL1-991
Salmo salar
Online Access:https://doi.org/10.3390/ani13203191
https://doaj.org/article/c71a2677bc26455e9479f8f0e44c8212
Description
Summary:The newly discovered IgT + B cell is thought to play a dominant role in mucosal immunity, but limited studies have examined its distribution in fish species, hindering our understanding of its function. This study investigated IgT and poly Ig receptor (pIgR) mRNA + cell distribution in Atlantic salmon ( Salmo salar ) gut using RNAscope in situ hybridization (ISH) and assessed the effects of vaccination. The pyloric caeca, mid-intestine (first and second parts), and posterior segment in two weight stages (Group 1: avg. 153 g, Group 2: avg. 1717 g) were examined in both vaccinated and unvaccinated fish. ISH revealed more IgT mRNA + cells in the second part of the midgut compared to other intestinal segments, as well as a higher number of positive cells in Group 2 (older fish). In line with previous findings, intraperitoneal vaccination had no significant impact on the number of IgT + transcripts. IgT mRNA + cells were found mostly in the lamina propria and near capillaries, while pIgR was registered in both the lamina propria and mucosa. Interestingly, vaccinated fish presented adhesions and granulomatous tissue in the peritoneum, with both IgT and pIgR mRNA + cells. Taken together, these results suggest that the distribution of IgT mRNA + cells in the intestine of Atlantic salmon is region-specific and is not affected by intraperitoneal vaccination but varies with fish age.

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