Differential circadian and light-driven rhythmicity of clock gene expression and behaviour in the turbot, Scophthalmus maximus.

In fish, the circadian clock represents a key regulator of many aspects of biology and is controlled by combinations of abiotic and biotic factors. These environmental factors are frequently manipulated in fish farms as part of strategies designed to maximize productivity. The flatfish turbot, Scoph...

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Published in:PLOS ONE
Main Authors: Rosa M Ceinos, Mauro Chivite, Marcos A López-Patiño, Fatemeh Naderi, José L Soengas, Nicholas S Foulkes, Jesús M Míguez
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science (PLoS) 2019
Subjects:
R
Q
Online Access:https://doi.org/10.1371/journal.pone.0219153
https://doaj.org/article/c6fb7eab8e7e4b83aabf144b47d6ae9b
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spelling ftdoajarticles:oai:doaj.org/article:c6fb7eab8e7e4b83aabf144b47d6ae9b 2023-05-15T18:15:46+02:00 Differential circadian and light-driven rhythmicity of clock gene expression and behaviour in the turbot, Scophthalmus maximus. Rosa M Ceinos Mauro Chivite Marcos A López-Patiño Fatemeh Naderi José L Soengas Nicholas S Foulkes Jesús M Míguez 2019-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0219153 https://doaj.org/article/c6fb7eab8e7e4b83aabf144b47d6ae9b EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0219153 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0219153 https://doaj.org/article/c6fb7eab8e7e4b83aabf144b47d6ae9b PLoS ONE, Vol 14, Iss 7, p e0219153 (2019) Medicine R Science Q article 2019 ftdoajarticles https://doi.org/10.1371/journal.pone.0219153 2022-12-31T05:54:25Z In fish, the circadian clock represents a key regulator of many aspects of biology and is controlled by combinations of abiotic and biotic factors. These environmental factors are frequently manipulated in fish farms as part of strategies designed to maximize productivity. The flatfish turbot, Scophthalmus maximus, represents one of the most important species within the aquaculture sector in Asia and Europe. Despite the strategic importance of this species, the function and regulation of the turbot circadian system remains poorly understood. Here, we have characterized the core circadian clock genes, clock1, per1, per2 and cry1 in turbot and have studied their daily expression in various tissues under a range of lighting conditions and feeding regimes. We have also explored the influence of light and feeding time on locomotor activity. Rhythmic expression of the four core clock genes was observed in all tissues studied under light dark (LD) cycle conditions. Rhythmicity of clock gene expression persisted upon transfer to artificial free running, constant conditions confirming their endogenous circadian clock control. Furthermore, turbot showed daily cycles of locomotor activity and food anticipatory activity (FAA) under LD and scheduled-feeding, with the activity phase as well as FAA coinciding with and being dependent upon exposure to light. Thus, while FAA was absent under constant dark (DD) conditions, it was still detected in constant light (LL). In contrast, general locomotor activity was arrhythmic in both constant darkness and constant light, pointing to a major contribution of light, in concert with the circadian clock, in timing locomotor activity in this species. Our data represents an important contribution to our understanding of the circadian timing system in the turbot and thereby the optimization of rearing protocols and the improvement of the well-being of turbot within fish farming environments. Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles PLOS ONE 14 7 e0219153
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Rosa M Ceinos
Mauro Chivite
Marcos A López-Patiño
Fatemeh Naderi
José L Soengas
Nicholas S Foulkes
Jesús M Míguez
Differential circadian and light-driven rhythmicity of clock gene expression and behaviour in the turbot, Scophthalmus maximus.
topic_facet Medicine
R
Science
Q
description In fish, the circadian clock represents a key regulator of many aspects of biology and is controlled by combinations of abiotic and biotic factors. These environmental factors are frequently manipulated in fish farms as part of strategies designed to maximize productivity. The flatfish turbot, Scophthalmus maximus, represents one of the most important species within the aquaculture sector in Asia and Europe. Despite the strategic importance of this species, the function and regulation of the turbot circadian system remains poorly understood. Here, we have characterized the core circadian clock genes, clock1, per1, per2 and cry1 in turbot and have studied their daily expression in various tissues under a range of lighting conditions and feeding regimes. We have also explored the influence of light and feeding time on locomotor activity. Rhythmic expression of the four core clock genes was observed in all tissues studied under light dark (LD) cycle conditions. Rhythmicity of clock gene expression persisted upon transfer to artificial free running, constant conditions confirming their endogenous circadian clock control. Furthermore, turbot showed daily cycles of locomotor activity and food anticipatory activity (FAA) under LD and scheduled-feeding, with the activity phase as well as FAA coinciding with and being dependent upon exposure to light. Thus, while FAA was absent under constant dark (DD) conditions, it was still detected in constant light (LL). In contrast, general locomotor activity was arrhythmic in both constant darkness and constant light, pointing to a major contribution of light, in concert with the circadian clock, in timing locomotor activity in this species. Our data represents an important contribution to our understanding of the circadian timing system in the turbot and thereby the optimization of rearing protocols and the improvement of the well-being of turbot within fish farming environments.
format Article in Journal/Newspaper
author Rosa M Ceinos
Mauro Chivite
Marcos A López-Patiño
Fatemeh Naderi
José L Soengas
Nicholas S Foulkes
Jesús M Míguez
author_facet Rosa M Ceinos
Mauro Chivite
Marcos A López-Patiño
Fatemeh Naderi
José L Soengas
Nicholas S Foulkes
Jesús M Míguez
author_sort Rosa M Ceinos
title Differential circadian and light-driven rhythmicity of clock gene expression and behaviour in the turbot, Scophthalmus maximus.
title_short Differential circadian and light-driven rhythmicity of clock gene expression and behaviour in the turbot, Scophthalmus maximus.
title_full Differential circadian and light-driven rhythmicity of clock gene expression and behaviour in the turbot, Scophthalmus maximus.
title_fullStr Differential circadian and light-driven rhythmicity of clock gene expression and behaviour in the turbot, Scophthalmus maximus.
title_full_unstemmed Differential circadian and light-driven rhythmicity of clock gene expression and behaviour in the turbot, Scophthalmus maximus.
title_sort differential circadian and light-driven rhythmicity of clock gene expression and behaviour in the turbot, scophthalmus maximus.
publisher Public Library of Science (PLoS)
publishDate 2019
url https://doi.org/10.1371/journal.pone.0219153
https://doaj.org/article/c6fb7eab8e7e4b83aabf144b47d6ae9b
genre Scophthalmus maximus
Turbot
genre_facet Scophthalmus maximus
Turbot
op_source PLoS ONE, Vol 14, Iss 7, p e0219153 (2019)
op_relation https://doi.org/10.1371/journal.pone.0219153
https://doaj.org/toc/1932-6203
1932-6203
doi:10.1371/journal.pone.0219153
https://doaj.org/article/c6fb7eab8e7e4b83aabf144b47d6ae9b
op_doi https://doi.org/10.1371/journal.pone.0219153
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