Molecular analysis and dimorphism of azole-susceptible and resistant Candida albicans isolates
INTRODUCTION: Candida albicans is responsible for superficial or systemic infections known as candidiasis, which may be found in infected tissue as unicellular budding yeasts, hyphae, or pseudohyphae. In this study, the effects of both fluconazole and itraconazole antifungal agents on the hyphal for...
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ftdoajarticles:oai:doaj.org/article:c6e3ccf59bfb444bbbe0df8595480430 2023-05-15T15:12:59+02:00 Molecular analysis and dimorphism of azole-susceptible and resistant Candida albicans isolates Carolina Rodrigues Costa Lúcia Kioko Hasimoto e Souza Fábio Silvestre Ataídes Pedro Henrique Ferri Maysa Paula da Costa Orionalda de Fátima Lisboa Fernanades Maria do Rosário Rodrigues Silva 2011-12-01T00:00:00Z https://doi.org/10.1590/s0037-86822011005000056 https://doaj.org/article/c6e3ccf59bfb444bbbe0df8595480430 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600017&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000056 https://doaj.org/article/c6e3ccf59bfb444bbbe0df8595480430 Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 6, Pp 740-744 (2011) Candida albicans Antifúngicos azólicos Formação hifal Suscetibilidade in vitro RAPD-PCR Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1590/s0037-86822011005000056 2022-12-30T23:24:04Z INTRODUCTION: Candida albicans is responsible for superficial or systemic infections known as candidiasis, which may be found in infected tissue as unicellular budding yeasts, hyphae, or pseudohyphae. In this study, the effects of both fluconazole and itraconazole antifungal agents on the hyphal formation and genotypic characterization of C. albicans isolates classified as either susceptible or resistant were investigated. METHODS: The hyphal production of five C. albicans isolates under the action of antifungal agents was investigated by culturing yeast on growth medium and on hyphal induction medium. The genotypic characterization was carried out for 13 isolates of C. albicans using the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) method. RESULTS: The dimorphism analysis showed that the hyphal formation was higher in resistant than in the susceptible isolates to both azoles. The RAPD-PCR method identified the formation of two different groups. In group A, four resistant and two susceptible isolates were clustered, and in group B, one resistant and six susceptible isolates were clustered. CONCLUSIONS: Considering that hyphal formation was higher in resistant isolates in the presence of azole drugs, we confirmed that the hyphal production is closely related to susceptibility to azoles. These drugs may affect the morphogenesis of C. albicans depending on their susceptibility to these drugs. In relation to RAPD-PCR, most resistant isolates classified in group A and susceptible isolates in group B demonstrated that this method presented a similar standard between the two groups, suggesting that by this technique, a strong correlation between genotypes and fluconazole-resistant samples may be found. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 44 6 740 744 |
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Candida albicans Antifúngicos azólicos Formação hifal Suscetibilidade in vitro RAPD-PCR Arctic medicine. Tropical medicine RC955-962 |
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Candida albicans Antifúngicos azólicos Formação hifal Suscetibilidade in vitro RAPD-PCR Arctic medicine. Tropical medicine RC955-962 Carolina Rodrigues Costa Lúcia Kioko Hasimoto e Souza Fábio Silvestre Ataídes Pedro Henrique Ferri Maysa Paula da Costa Orionalda de Fátima Lisboa Fernanades Maria do Rosário Rodrigues Silva Molecular analysis and dimorphism of azole-susceptible and resistant Candida albicans isolates |
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Candida albicans Antifúngicos azólicos Formação hifal Suscetibilidade in vitro RAPD-PCR Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Candida albicans is responsible for superficial or systemic infections known as candidiasis, which may be found in infected tissue as unicellular budding yeasts, hyphae, or pseudohyphae. In this study, the effects of both fluconazole and itraconazole antifungal agents on the hyphal formation and genotypic characterization of C. albicans isolates classified as either susceptible or resistant were investigated. METHODS: The hyphal production of five C. albicans isolates under the action of antifungal agents was investigated by culturing yeast on growth medium and on hyphal induction medium. The genotypic characterization was carried out for 13 isolates of C. albicans using the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) method. RESULTS: The dimorphism analysis showed that the hyphal formation was higher in resistant than in the susceptible isolates to both azoles. The RAPD-PCR method identified the formation of two different groups. In group A, four resistant and two susceptible isolates were clustered, and in group B, one resistant and six susceptible isolates were clustered. CONCLUSIONS: Considering that hyphal formation was higher in resistant isolates in the presence of azole drugs, we confirmed that the hyphal production is closely related to susceptibility to azoles. These drugs may affect the morphogenesis of C. albicans depending on their susceptibility to these drugs. In relation to RAPD-PCR, most resistant isolates classified in group A and susceptible isolates in group B demonstrated that this method presented a similar standard between the two groups, suggesting that by this technique, a strong correlation between genotypes and fluconazole-resistant samples may be found. |
format |
Article in Journal/Newspaper |
author |
Carolina Rodrigues Costa Lúcia Kioko Hasimoto e Souza Fábio Silvestre Ataídes Pedro Henrique Ferri Maysa Paula da Costa Orionalda de Fátima Lisboa Fernanades Maria do Rosário Rodrigues Silva |
author_facet |
Carolina Rodrigues Costa Lúcia Kioko Hasimoto e Souza Fábio Silvestre Ataídes Pedro Henrique Ferri Maysa Paula da Costa Orionalda de Fátima Lisboa Fernanades Maria do Rosário Rodrigues Silva |
author_sort |
Carolina Rodrigues Costa |
title |
Molecular analysis and dimorphism of azole-susceptible and resistant Candida albicans isolates |
title_short |
Molecular analysis and dimorphism of azole-susceptible and resistant Candida albicans isolates |
title_full |
Molecular analysis and dimorphism of azole-susceptible and resistant Candida albicans isolates |
title_fullStr |
Molecular analysis and dimorphism of azole-susceptible and resistant Candida albicans isolates |
title_full_unstemmed |
Molecular analysis and dimorphism of azole-susceptible and resistant Candida albicans isolates |
title_sort |
molecular analysis and dimorphism of azole-susceptible and resistant candida albicans isolates |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doi.org/10.1590/s0037-86822011005000056 https://doaj.org/article/c6e3ccf59bfb444bbbe0df8595480430 |
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Arctic |
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Arctic |
genre |
Arctic |
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Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 6, Pp 740-744 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600017&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/s0037-86822011005000056 https://doaj.org/article/c6e3ccf59bfb444bbbe0df8595480430 |
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https://doi.org/10.1590/s0037-86822011005000056 |
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44 |
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