Local increase of arginase activity in lesions of patients with cutaneous leishmaniasis in Ethiopia.
Cutaneous leishmaniasis is a vector-borne disease that is in Ethiopia mainly caused by the parasite Leishmania aethiopica. This neglected tropical disease is common in rural areas and causes serious morbidity. Persistent nonhealing cutaneous leishmaniasis has been associated with poor T cell mediate...
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ftdoajarticles:oai:doaj.org/article:c6af55791b7c43f3b862a69e1a7f587e 2023-05-15T15:09:05+02:00 Local increase of arginase activity in lesions of patients with cutaneous leishmaniasis in Ethiopia. Tamrat Abebe Asrat Hailu Mihretu Woldeyes Woinshet Mekonen Kassahun Bilcha Thomas Cloke Lionel Fry Nafisa-Katrin Seich Al Basatena Karina Corware Manuel Modolell Markus Munder Fabienne Tacchini-Cottier Ingrid Müller Pascale Kropf 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001684 https://doaj.org/article/c6af55791b7c43f3b862a69e1a7f587e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3373636?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001684 https://doaj.org/article/c6af55791b7c43f3b862a69e1a7f587e PLoS Neglected Tropical Diseases, Vol 6, Iss 6, p e1684 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001684 2022-12-31T10:36:22Z Cutaneous leishmaniasis is a vector-borne disease that is in Ethiopia mainly caused by the parasite Leishmania aethiopica. This neglected tropical disease is common in rural areas and causes serious morbidity. Persistent nonhealing cutaneous leishmaniasis has been associated with poor T cell mediated responses; however, the underlying mechanisms are not well understood.We have recently shown in an experimental model of cutaneous leishmaniasis that arginase-induced L-arginine metabolism suppresses antigen-specific T cell responses at the site of pathology, but not in the periphery. To test whether these results translate to human disease, we recruited patients presenting with localized lesions of cutaneous leishmaniasis and assessed the levels of arginase activity in cells isolated from peripheral blood and from skin biopsies. Arginase activity was similar in peripheral blood mononuclear cells (PBMCs) from patients and healthy controls. In sharp contrast, arginase activity was significantly increased in lesion biopsies of patients with localized cutaneous leishmaniasis as compared with controls. Furthermore, we found that the expression levels of CD3ζ, CD4 and CD8 molecules were considerably lower at the site of pathology as compared to those observed in paired PBMCs.Our results suggest that increased arginase in lesions of patients with cutaneous leishmaniasis might play a role in the pathogenesis of the disease by impairing T cell effector functions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 6 e1684 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Tamrat Abebe Asrat Hailu Mihretu Woldeyes Woinshet Mekonen Kassahun Bilcha Thomas Cloke Lionel Fry Nafisa-Katrin Seich Al Basatena Karina Corware Manuel Modolell Markus Munder Fabienne Tacchini-Cottier Ingrid Müller Pascale Kropf Local increase of arginase activity in lesions of patients with cutaneous leishmaniasis in Ethiopia. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Cutaneous leishmaniasis is a vector-borne disease that is in Ethiopia mainly caused by the parasite Leishmania aethiopica. This neglected tropical disease is common in rural areas and causes serious morbidity. Persistent nonhealing cutaneous leishmaniasis has been associated with poor T cell mediated responses; however, the underlying mechanisms are not well understood.We have recently shown in an experimental model of cutaneous leishmaniasis that arginase-induced L-arginine metabolism suppresses antigen-specific T cell responses at the site of pathology, but not in the periphery. To test whether these results translate to human disease, we recruited patients presenting with localized lesions of cutaneous leishmaniasis and assessed the levels of arginase activity in cells isolated from peripheral blood and from skin biopsies. Arginase activity was similar in peripheral blood mononuclear cells (PBMCs) from patients and healthy controls. In sharp contrast, arginase activity was significantly increased in lesion biopsies of patients with localized cutaneous leishmaniasis as compared with controls. Furthermore, we found that the expression levels of CD3ζ, CD4 and CD8 molecules were considerably lower at the site of pathology as compared to those observed in paired PBMCs.Our results suggest that increased arginase in lesions of patients with cutaneous leishmaniasis might play a role in the pathogenesis of the disease by impairing T cell effector functions. |
format |
Article in Journal/Newspaper |
author |
Tamrat Abebe Asrat Hailu Mihretu Woldeyes Woinshet Mekonen Kassahun Bilcha Thomas Cloke Lionel Fry Nafisa-Katrin Seich Al Basatena Karina Corware Manuel Modolell Markus Munder Fabienne Tacchini-Cottier Ingrid Müller Pascale Kropf |
author_facet |
Tamrat Abebe Asrat Hailu Mihretu Woldeyes Woinshet Mekonen Kassahun Bilcha Thomas Cloke Lionel Fry Nafisa-Katrin Seich Al Basatena Karina Corware Manuel Modolell Markus Munder Fabienne Tacchini-Cottier Ingrid Müller Pascale Kropf |
author_sort |
Tamrat Abebe |
title |
Local increase of arginase activity in lesions of patients with cutaneous leishmaniasis in Ethiopia. |
title_short |
Local increase of arginase activity in lesions of patients with cutaneous leishmaniasis in Ethiopia. |
title_full |
Local increase of arginase activity in lesions of patients with cutaneous leishmaniasis in Ethiopia. |
title_fullStr |
Local increase of arginase activity in lesions of patients with cutaneous leishmaniasis in Ethiopia. |
title_full_unstemmed |
Local increase of arginase activity in lesions of patients with cutaneous leishmaniasis in Ethiopia. |
title_sort |
local increase of arginase activity in lesions of patients with cutaneous leishmaniasis in ethiopia. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001684 https://doaj.org/article/c6af55791b7c43f3b862a69e1a7f587e |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 6, p e1684 (2012) |
op_relation |
http://europepmc.org/articles/PMC3373636?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001684 https://doaj.org/article/c6af55791b7c43f3b862a69e1a7f587e |
op_doi |
https://doi.org/10.1371/journal.pntd.0001684 |
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PLoS Neglected Tropical Diseases |
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6 |
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6 |
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e1684 |
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