Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress
Objective: To investigate the effect of the hexane solvent fraction of Halymenia durvillei (HDHE) on triple-negative breast cancer. Methods: The phytochemical profile of HDHE was investigated by GC-MS. The cytotoxicity of HDHE against MDA-MB-231 cells was determined. The apoptotic and autophagic eff...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2022
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Online Access: | https://doi.org/10.4103/2221-1691.338922 https://doaj.org/article/c6aac27771364750a11c5291e51bfc6b |
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ftdoajarticles:oai:doaj.org/article:c6aac27771364750a11c5291e51bfc6b 2023-05-15T15:07:38+02:00 Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress Kant Sangpairoj Rapeewan Settacomkul Tanapan Siangcham Krai Meemon Nakorn Niamnont Nilubon Sornkaew Montakan Tamtin Prasert Sobhon Pornpun Vivithanaporn 2022-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.338922 https://doaj.org/article/c6aac27771364750a11c5291e51bfc6b EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=3;spage=132;epage=140;aulast=Sangpairoj https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.338922 https://doaj.org/article/c6aac27771364750a11c5291e51bfc6b Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 3, Pp 132-140 (2022) halymenia durvillei triple-negative breast cancer apoptosis autophagy endoplasmic reticulum stress red algae hexadecanoic acid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.4103/2221-1691.338922 2022-12-30T21:33:07Z Objective: To investigate the effect of the hexane solvent fraction of Halymenia durvillei (HDHE) on triple-negative breast cancer. Methods: The phytochemical profile of HDHE was investigated by GC-MS. The cytotoxicity of HDHE against MDA-MB-231 cells was determined. The apoptotic and autophagic effects of HDHE were analyzed. The expression of molecular markers controlling apoptosis, autophagy, DNA damage, and endoplasmic reticulum (ER) stress was determined. Results: HDHE contains a mixture of fatty acids, mainly hexadecanoic acid. HDHE at a cytotoxic concentration induced apoptotic death of MDA-MB-231 cells through mitochondrial membrane dysfunction, and induction of apoptosis markers, and increased the expression of proteins related to DNA damage response. HDHE also induced the expression of LC-3, a marker of autophagic cell death at a cytotoxic concentration. Moreover, HDHE modulated the expression of ER stress genes. Conclusions: The hexadecanoic acid-enriched extract of Halymenia durvillei promotes apoptosis and autophagy of human triple-negative breast cancer cells. This extract may be further explored as an anticancer agent for triple-negative breast cancer. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 12 3 132 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
halymenia durvillei triple-negative breast cancer apoptosis autophagy endoplasmic reticulum stress red algae hexadecanoic acid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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halymenia durvillei triple-negative breast cancer apoptosis autophagy endoplasmic reticulum stress red algae hexadecanoic acid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Kant Sangpairoj Rapeewan Settacomkul Tanapan Siangcham Krai Meemon Nakorn Niamnont Nilubon Sornkaew Montakan Tamtin Prasert Sobhon Pornpun Vivithanaporn Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress |
topic_facet |
halymenia durvillei triple-negative breast cancer apoptosis autophagy endoplasmic reticulum stress red algae hexadecanoic acid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the effect of the hexane solvent fraction of Halymenia durvillei (HDHE) on triple-negative breast cancer. Methods: The phytochemical profile of HDHE was investigated by GC-MS. The cytotoxicity of HDHE against MDA-MB-231 cells was determined. The apoptotic and autophagic effects of HDHE were analyzed. The expression of molecular markers controlling apoptosis, autophagy, DNA damage, and endoplasmic reticulum (ER) stress was determined. Results: HDHE contains a mixture of fatty acids, mainly hexadecanoic acid. HDHE at a cytotoxic concentration induced apoptotic death of MDA-MB-231 cells through mitochondrial membrane dysfunction, and induction of apoptosis markers, and increased the expression of proteins related to DNA damage response. HDHE also induced the expression of LC-3, a marker of autophagic cell death at a cytotoxic concentration. Moreover, HDHE modulated the expression of ER stress genes. Conclusions: The hexadecanoic acid-enriched extract of Halymenia durvillei promotes apoptosis and autophagy of human triple-negative breast cancer cells. This extract may be further explored as an anticancer agent for triple-negative breast cancer. |
format |
Article in Journal/Newspaper |
author |
Kant Sangpairoj Rapeewan Settacomkul Tanapan Siangcham Krai Meemon Nakorn Niamnont Nilubon Sornkaew Montakan Tamtin Prasert Sobhon Pornpun Vivithanaporn |
author_facet |
Kant Sangpairoj Rapeewan Settacomkul Tanapan Siangcham Krai Meemon Nakorn Niamnont Nilubon Sornkaew Montakan Tamtin Prasert Sobhon Pornpun Vivithanaporn |
author_sort |
Kant Sangpairoj |
title |
Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress |
title_short |
Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress |
title_full |
Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress |
title_fullStr |
Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress |
title_full_unstemmed |
Hexadecanoic acid-enriched extract of Halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating ER stress |
title_sort |
hexadecanoic acid-enriched extract of halymenia durvillei induces apoptotic and autophagic death of human triple-negative breast cancer cells by upregulating er stress |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
url |
https://doi.org/10.4103/2221-1691.338922 https://doaj.org/article/c6aac27771364750a11c5291e51bfc6b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 3, Pp 132-140 (2022) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=3;spage=132;epage=140;aulast=Sangpairoj https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.338922 https://doaj.org/article/c6aac27771364750a11c5291e51bfc6b |
op_doi |
https://doi.org/10.4103/2221-1691.338922 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
12 |
container_issue |
3 |
container_start_page |
132 |
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1766339091448725504 |