Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells
The acute phase of Chagas' disease in mice and human is marked by states of immunosuppression, in which Trypanosoma cruzi replicates extensively and releases immunomodulatory molecules that delay parasite-specific responses mediated by effector T cells. This mechanism of evasion allows the para...
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ftdoajarticles:oai:doaj.org/article:c66214cef7a446a2a308e711abf69f75 2024-09-09T19:25:37+00:00 Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells Yadira Morán-Utrera Aracely López-Monteon José Luis Rosales-Encina Enrique Méndez-Bolaina Angel Ramos-Ligonio 2012-01-01T00:00:00Z https://doi.org/10.1155/2012/829139 https://doaj.org/article/c66214cef7a446a2a308e711abf69f75 EN eng Wiley http://dx.doi.org/10.1155/2012/829139 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2012/829139 https://doaj.org/article/c66214cef7a446a2a308e711abf69f75 Journal of Tropical Medicine, Vol 2012 (2012) Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1155/2012/829139 2024-08-05T17:48:34Z The acute phase of Chagas' disease in mice and human is marked by states of immunosuppression, in which Trypanosoma cruzi replicates extensively and releases immunomodulatory molecules that delay parasite-specific responses mediated by effector T cells. This mechanism of evasion allows the parasite to spread in the host. Parasite molecules that regulate the host immune response during Chagas’ disease have not been fully identified, particularly proteins of the amastigote stage. In this work, we evaluated the role of the GPI anchored SSP4 protein of T. cruzi as an immunomodulatory molecule in peripheral blood mononuclear cells (PBMCs). rMBP::SSP4 protein was able to stimulate nitric oxide (NO) production. Likewise, rMBP::SSP4 induced the expression of genes and production of molecules involved in the inflammatory process, such as, cytokines, chemokines, and adhesion molecules (CAMs) as determined by RT-PCR and ELISA. These results suggest that the amastigote SSP4 molecule could play a key role in the immunoregulatory and/or immunosuppressive process observed in the acute phase of infection with T. cruzi. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2012 1 10 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Yadira Morán-Utrera Aracely López-Monteon José Luis Rosales-Encina Enrique Méndez-Bolaina Angel Ramos-Ligonio Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
The acute phase of Chagas' disease in mice and human is marked by states of immunosuppression, in which Trypanosoma cruzi replicates extensively and releases immunomodulatory molecules that delay parasite-specific responses mediated by effector T cells. This mechanism of evasion allows the parasite to spread in the host. Parasite molecules that regulate the host immune response during Chagas’ disease have not been fully identified, particularly proteins of the amastigote stage. In this work, we evaluated the role of the GPI anchored SSP4 protein of T. cruzi as an immunomodulatory molecule in peripheral blood mononuclear cells (PBMCs). rMBP::SSP4 protein was able to stimulate nitric oxide (NO) production. Likewise, rMBP::SSP4 induced the expression of genes and production of molecules involved in the inflammatory process, such as, cytokines, chemokines, and adhesion molecules (CAMs) as determined by RT-PCR and ELISA. These results suggest that the amastigote SSP4 molecule could play a key role in the immunoregulatory and/or immunosuppressive process observed in the acute phase of infection with T. cruzi. |
format |
Article in Journal/Newspaper |
author |
Yadira Morán-Utrera Aracely López-Monteon José Luis Rosales-Encina Enrique Méndez-Bolaina Angel Ramos-Ligonio |
author_facet |
Yadira Morán-Utrera Aracely López-Monteon José Luis Rosales-Encina Enrique Méndez-Bolaina Angel Ramos-Ligonio |
author_sort |
Yadira Morán-Utrera |
title |
Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
title_short |
Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
title_full |
Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
title_fullStr |
Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
title_full_unstemmed |
Trypanosoma cruzi SSP4 Amastigote Protein Induces Expression of Immunoregulatory and Immunosuppressive Molecules in Peripheral Blood Mononuclear Cells |
title_sort |
trypanosoma cruzi ssp4 amastigote protein induces expression of immunoregulatory and immunosuppressive molecules in peripheral blood mononuclear cells |
publisher |
Wiley |
publishDate |
2012 |
url |
https://doi.org/10.1155/2012/829139 https://doaj.org/article/c66214cef7a446a2a308e711abf69f75 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2012 (2012) |
op_relation |
http://dx.doi.org/10.1155/2012/829139 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2012/829139 https://doaj.org/article/c66214cef7a446a2a308e711abf69f75 |
op_doi |
https://doi.org/10.1155/2012/829139 |
container_title |
Journal of Tropical Medicine |
container_volume |
2012 |
container_start_page |
1 |
op_container_end_page |
10 |
_version_ |
1809895370689347584 |