Transcriptomic Insight into the Melon Morphology of Toothed Whales for Aquatic Molecular Developments
Aquatic habitats are home to large animals such as marine mammals. Toothed whales have special fat deposits in the forehead region (called the melon ) of their heads that are associated with echolocation underwater. This fat is also important industrially for human use. Due to the lack of gene expre...
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ftdoajarticles:oai:doaj.org/article:c65f89079ed344eaabbb6ebad70767c3 2023-05-15T18:33:28+02:00 Transcriptomic Insight into the Melon Morphology of Toothed Whales for Aquatic Molecular Developments Jayan Duminda Mahesh Senevirathna Ryo Yonezawa Taiki Saka Yoji Igarashi Noriko Funasaka Kazutoshi Yoshitake Shigeharu Kinoshita Shuichi Asakawa 2021-12-01T00:00:00Z https://doi.org/10.3390/su132413997 https://doaj.org/article/c65f89079ed344eaabbb6ebad70767c3 EN eng MDPI AG https://www.mdpi.com/2071-1050/13/24/13997 https://doaj.org/toc/2071-1050 doi:10.3390/su132413997 2071-1050 https://doaj.org/article/c65f89079ed344eaabbb6ebad70767c3 Sustainability, Vol 13, Iss 13997, p 13997 (2021) lipids transcriptomics gene expression fat biosynthesis marine mammals Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 article 2021 ftdoajarticles https://doi.org/10.3390/su132413997 2022-12-31T12:46:27Z Aquatic habitats are home to large animals such as marine mammals. Toothed whales have special fat deposits in the forehead region (called the melon ) of their heads that are associated with echolocation underwater. This fat is also important industrially for human use. Due to the lack of gene expression information on the melon fat of toothed whales, we investigated the melon morphology via the transcriptomic approach. Four parts of the melons of three individual Risso’s dolphins were used for total RNA extraction, cDNA library preparation, and sequencing via next-generation sequencing (NGS) technologies. After the downstream analysis of raw sequence data, we determined that the outer layer of the melon’s ML4 region played multifunctional roles. The 36 differentially expressed genes of outer melon included ASB5 , MYH13 , MYOM2 , and MYOM3 . These genes are associated with muscle function and energy metabolism. Gene clustering and functional enrichment analyses also represented enrichments, such as the pentose phosphate pathway and morphogenesis related to lipid metabolism and muscle functions. This study will be crucial for muscle and fat functional-related molecular studies on aquatic mammals. Additionally, the study presents potential pathways, such as melon fat biosynthesis, for sustainable future developments. Article in Journal/Newspaper toothed whales Directory of Open Access Journals: DOAJ Articles Sustainability 13 24 13997 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
lipids transcriptomics gene expression fat biosynthesis marine mammals Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 |
spellingShingle |
lipids transcriptomics gene expression fat biosynthesis marine mammals Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 Jayan Duminda Mahesh Senevirathna Ryo Yonezawa Taiki Saka Yoji Igarashi Noriko Funasaka Kazutoshi Yoshitake Shigeharu Kinoshita Shuichi Asakawa Transcriptomic Insight into the Melon Morphology of Toothed Whales for Aquatic Molecular Developments |
topic_facet |
lipids transcriptomics gene expression fat biosynthesis marine mammals Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 |
description |
Aquatic habitats are home to large animals such as marine mammals. Toothed whales have special fat deposits in the forehead region (called the melon ) of their heads that are associated with echolocation underwater. This fat is also important industrially for human use. Due to the lack of gene expression information on the melon fat of toothed whales, we investigated the melon morphology via the transcriptomic approach. Four parts of the melons of three individual Risso’s dolphins were used for total RNA extraction, cDNA library preparation, and sequencing via next-generation sequencing (NGS) technologies. After the downstream analysis of raw sequence data, we determined that the outer layer of the melon’s ML4 region played multifunctional roles. The 36 differentially expressed genes of outer melon included ASB5 , MYH13 , MYOM2 , and MYOM3 . These genes are associated with muscle function and energy metabolism. Gene clustering and functional enrichment analyses also represented enrichments, such as the pentose phosphate pathway and morphogenesis related to lipid metabolism and muscle functions. This study will be crucial for muscle and fat functional-related molecular studies on aquatic mammals. Additionally, the study presents potential pathways, such as melon fat biosynthesis, for sustainable future developments. |
format |
Article in Journal/Newspaper |
author |
Jayan Duminda Mahesh Senevirathna Ryo Yonezawa Taiki Saka Yoji Igarashi Noriko Funasaka Kazutoshi Yoshitake Shigeharu Kinoshita Shuichi Asakawa |
author_facet |
Jayan Duminda Mahesh Senevirathna Ryo Yonezawa Taiki Saka Yoji Igarashi Noriko Funasaka Kazutoshi Yoshitake Shigeharu Kinoshita Shuichi Asakawa |
author_sort |
Jayan Duminda Mahesh Senevirathna |
title |
Transcriptomic Insight into the Melon Morphology of Toothed Whales for Aquatic Molecular Developments |
title_short |
Transcriptomic Insight into the Melon Morphology of Toothed Whales for Aquatic Molecular Developments |
title_full |
Transcriptomic Insight into the Melon Morphology of Toothed Whales for Aquatic Molecular Developments |
title_fullStr |
Transcriptomic Insight into the Melon Morphology of Toothed Whales for Aquatic Molecular Developments |
title_full_unstemmed |
Transcriptomic Insight into the Melon Morphology of Toothed Whales for Aquatic Molecular Developments |
title_sort |
transcriptomic insight into the melon morphology of toothed whales for aquatic molecular developments |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/su132413997 https://doaj.org/article/c65f89079ed344eaabbb6ebad70767c3 |
genre |
toothed whales |
genre_facet |
toothed whales |
op_source |
Sustainability, Vol 13, Iss 13997, p 13997 (2021) |
op_relation |
https://www.mdpi.com/2071-1050/13/24/13997 https://doaj.org/toc/2071-1050 doi:10.3390/su132413997 2071-1050 https://doaj.org/article/c65f89079ed344eaabbb6ebad70767c3 |
op_doi |
https://doi.org/10.3390/su132413997 |
container_title |
Sustainability |
container_volume |
13 |
container_issue |
24 |
container_start_page |
13997 |
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1766218082237284352 |