Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth
Helicobacter pylori (H. pylori) is a gram-negative bacterium that colonizes the human stomach. Infection with this microaerophilic bacterium causes gastric and duodenal ulcer. This study sought to isolate H. pylori, from gastric biopsy samples of dyspeptic patients in Ghana using a 2,3,5-triphenylte...
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2018
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ftdoajarticles:oai:doaj.org/article:c657a220ba8647d0a6bd25d775c6c9f5 2023-05-15T15:10:23+02:00 Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth Michael Buenor Adinortey Charles Ansah Cynthia Ayefoumi Adinortey Ansumana Sandy Bockarie Martin Tangnaa Morna Damian H. Amewowor 2018-01-01T00:00:00Z https://doi.org/10.1155/2018/8071081 https://doaj.org/article/c657a220ba8647d0a6bd25d775c6c9f5 EN eng Hindawi Limited http://dx.doi.org/10.1155/2018/8071081 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2018/8071081 https://doaj.org/article/c657a220ba8647d0a6bd25d775c6c9f5 Journal of Tropical Medicine, Vol 2018 (2018) Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.1155/2018/8071081 2022-12-31T00:09:48Z Helicobacter pylori (H. pylori) is a gram-negative bacterium that colonizes the human stomach. Infection with this microaerophilic bacterium causes gastric and duodenal ulcer. This study sought to isolate H. pylori, from gastric biopsy samples of dyspeptic patients in Ghana using a 2,3,5-triphenyltetrazolium chloride (TTC) dye incorporated medium method. This TTC dye method was further used in an antimicrobial susceptibility assay involving Dissotis rotundifolia extract (DRE). H. pylori were successfully isolated from gastric biopsy of dyspeptic patients. Pure cultures of H. pylori in 2,3,5-triphenyltetrazolium chloride (TTC) dye incorporated medium were seen as sparkling colonies. Isolates, identified as H. pylori, were gram-negative and urease, catalase, and oxidase positive and showed characteristic morphology as spiral-shaped bacteria under the microscope. The organisms were found to be susceptible to cephalothin and resistant to nalidixic acid. Above all, the observation that H. pylori grew only at 37°C and not 25°C or 42°C affirms that the bacterium is neither Helicobacter cinaedi nor Helicobacter fenneliae. The anti-H. pylori study depicts a statistically lower zone of inhibition for DRE compared to standard drugs [amoxicillin and clarithromycin] (p<0.05), whereas metronidazole showed no zone of inhibition. This study reports the first successful isolation and culturing of H. pylori in Ghana using TTC dye. It also shows that DRE possess an in vitro anti-H. pylori activity and that DRE has some therapeutic potential against H. pylori infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2018 1 6 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
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Arctic medicine. Tropical medicine RC955-962 Michael Buenor Adinortey Charles Ansah Cynthia Ayefoumi Adinortey Ansumana Sandy Bockarie Martin Tangnaa Morna Damian H. Amewowor Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Helicobacter pylori (H. pylori) is a gram-negative bacterium that colonizes the human stomach. Infection with this microaerophilic bacterium causes gastric and duodenal ulcer. This study sought to isolate H. pylori, from gastric biopsy samples of dyspeptic patients in Ghana using a 2,3,5-triphenyltetrazolium chloride (TTC) dye incorporated medium method. This TTC dye method was further used in an antimicrobial susceptibility assay involving Dissotis rotundifolia extract (DRE). H. pylori were successfully isolated from gastric biopsy of dyspeptic patients. Pure cultures of H. pylori in 2,3,5-triphenyltetrazolium chloride (TTC) dye incorporated medium were seen as sparkling colonies. Isolates, identified as H. pylori, were gram-negative and urease, catalase, and oxidase positive and showed characteristic morphology as spiral-shaped bacteria under the microscope. The organisms were found to be susceptible to cephalothin and resistant to nalidixic acid. Above all, the observation that H. pylori grew only at 37°C and not 25°C or 42°C affirms that the bacterium is neither Helicobacter cinaedi nor Helicobacter fenneliae. The anti-H. pylori study depicts a statistically lower zone of inhibition for DRE compared to standard drugs [amoxicillin and clarithromycin] (p<0.05), whereas metronidazole showed no zone of inhibition. This study reports the first successful isolation and culturing of H. pylori in Ghana using TTC dye. It also shows that DRE possess an in vitro anti-H. pylori activity and that DRE has some therapeutic potential against H. pylori infection. |
format |
Article in Journal/Newspaper |
author |
Michael Buenor Adinortey Charles Ansah Cynthia Ayefoumi Adinortey Ansumana Sandy Bockarie Martin Tangnaa Morna Damian H. Amewowor |
author_facet |
Michael Buenor Adinortey Charles Ansah Cynthia Ayefoumi Adinortey Ansumana Sandy Bockarie Martin Tangnaa Morna Damian H. Amewowor |
author_sort |
Michael Buenor Adinortey |
title |
Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth |
title_short |
Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth |
title_full |
Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth |
title_fullStr |
Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth |
title_full_unstemmed |
Isolation of Helicobacter pylori from Gastric Biopsy of Dyspeptic Patients in Ghana and In Vitro Preliminary Assessment of the Effect of Dissotis rotundifolia Extract on Its Growth |
title_sort |
isolation of helicobacter pylori from gastric biopsy of dyspeptic patients in ghana and in vitro preliminary assessment of the effect of dissotis rotundifolia extract on its growth |
publisher |
Hindawi Limited |
publishDate |
2018 |
url |
https://doi.org/10.1155/2018/8071081 https://doaj.org/article/c657a220ba8647d0a6bd25d775c6c9f5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2018 (2018) |
op_relation |
http://dx.doi.org/10.1155/2018/8071081 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2018/8071081 https://doaj.org/article/c657a220ba8647d0a6bd25d775c6c9f5 |
op_doi |
https://doi.org/10.1155/2018/8071081 |
container_title |
Journal of Tropical Medicine |
container_volume |
2018 |
container_start_page |
1 |
op_container_end_page |
6 |
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1766341423074902016 |