Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification
The knowledge of mosquitoes Culicidae host feeding patterns is basic to understand the roles of different species and to indicate their importance in the epidemiology of arthropod-borne diseases. A laboratory assay was developed aiming at standardizing the biotin-avidin sandwich enzyme-linked immuno...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:c65666ab0f614f8e962de0aefa831d6f 2023-05-15T15:06:04+02:00 Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification A. M. Marassá M. D. B. Rosa A. C. Gomes C. A. Consales 2008-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992008000200008 https://doaj.org/article/c65666ab0f614f8e962de0aefa831d6f EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200008 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992008000200008 1678-9199 https://doaj.org/article/c65666ab0f614f8e962de0aefa831d6f Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 14, Iss 2, Pp 303-312 (2008) biotin/avidin sandwich enzyme-linked immunosorbent assay Culicidae blood meal identification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2008 ftdoajarticles https://doi.org/10.1590/S1678-91992008000200008 2022-12-31T00:28:16Z The knowledge of mosquitoes Culicidae host feeding patterns is basic to understand the roles of different species and to indicate their importance in the epidemiology of arthropod-borne diseases. A laboratory assay was developed aiming at standardizing the biotin-avidin sandwich enzyme-linked immunosorbent assay, which was unprecedented for mosquito blood meal identification. The enzyme-linked immunosorbent assay (ELISA) activity was evaluated by the detection of titers on each sample of the 28 blood-fed Culex quinquefasciatus. In light of the high sensitivity that the technique permits, by means of small quantities of specific antibodies commercially provided and phosphatase substrate which reinforces additional dilutions, human and rat blood meals were readily identified in all laboratory-raised Culex quinquefasciatus tested. The assay was effective to detect human blood meal dilutions up to 1:4,096, which enables the technique to be applied in field studies. Additionally, the present results indicate a significant difference between the detection patterns recorded from human blood meal which corroborate the results of host feeding patterns. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 14 2 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
biotin/avidin sandwich enzyme-linked immunosorbent assay Culicidae blood meal identification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
biotin/avidin sandwich enzyme-linked immunosorbent assay Culicidae blood meal identification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 A. M. Marassá M. D. B. Rosa A. C. Gomes C. A. Consales Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
topic_facet |
biotin/avidin sandwich enzyme-linked immunosorbent assay Culicidae blood meal identification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The knowledge of mosquitoes Culicidae host feeding patterns is basic to understand the roles of different species and to indicate their importance in the epidemiology of arthropod-borne diseases. A laboratory assay was developed aiming at standardizing the biotin-avidin sandwich enzyme-linked immunosorbent assay, which was unprecedented for mosquito blood meal identification. The enzyme-linked immunosorbent assay (ELISA) activity was evaluated by the detection of titers on each sample of the 28 blood-fed Culex quinquefasciatus. In light of the high sensitivity that the technique permits, by means of small quantities of specific antibodies commercially provided and phosphatase substrate which reinforces additional dilutions, human and rat blood meals were readily identified in all laboratory-raised Culex quinquefasciatus tested. The assay was effective to detect human blood meal dilutions up to 1:4,096, which enables the technique to be applied in field studies. Additionally, the present results indicate a significant difference between the detection patterns recorded from human blood meal which corroborate the results of host feeding patterns. |
format |
Article in Journal/Newspaper |
author |
A. M. Marassá M. D. B. Rosa A. C. Gomes C. A. Consales |
author_facet |
A. M. Marassá M. D. B. Rosa A. C. Gomes C. A. Consales |
author_sort |
A. M. Marassá |
title |
Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
title_short |
Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
title_full |
Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
title_fullStr |
Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
title_full_unstemmed |
Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
title_sort |
biotin/avidin sandwich enzyme-linked immunosorbent assay for culicidae mosquito blood meal identification |
publisher |
SciELO |
publishDate |
2008 |
url |
https://doi.org/10.1590/S1678-91992008000200008 https://doaj.org/article/c65666ab0f614f8e962de0aefa831d6f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 14, Iss 2, Pp 303-312 (2008) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200008 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992008000200008 1678-9199 https://doaj.org/article/c65666ab0f614f8e962de0aefa831d6f |
op_doi |
https://doi.org/10.1590/S1678-91992008000200008 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
14 |
container_issue |
2 |
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1766337730323677184 |