Biofilm production and presence of genes icaABCD in Staphylococcus aureus strains isolated from raw milk
In the pathogenesis of staphylococcal mastitis the biofilm is considered a marker of virulence that allows the adhesion of the microorganism to the mammary epithelium. In order to determine the production of biofilm and the presence of icaABCD gene, 30 S. aureus strains isolated from raw milk obtain...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2017
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ftdoajarticles:oai:doaj.org/article:c64f009717ef484bb3474c3abda97299 2023-10-09T21:49:08+02:00 Biofilm production and presence of genes icaABCD in Staphylococcus aureus strains isolated from raw milk Messaria Ginestre P. Yeiny Ávila R. Kutchynskaya Valero L. Jhoandry Rivera S. Wilfrido Briñez Z. Robert Valeris C. 2017-12-01T00:00:00Z https://doaj.org/article/c64f009717ef484bb3474c3abda97299 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/23058 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/c64f009717ef484bb3474c3abda97299 Kasmera, Vol 45, Iss 2, Pp 79-87 (2017) Biofilm S. aureus genes ica leche cruda Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles 2023-09-10T00:49:12Z In the pathogenesis of staphylococcal mastitis the biofilm is considered a marker of virulence that allows the adhesion of the microorganism to the mammary epithelium. In order to determine the production of biofilm and the presence of icaABCD gene, 30 S. aureus strains isolated from raw milk obtained from cows with subclinical mastitis were analyzed. Biofilm production was determined by the quantitative method on microplates for cell culture and the presence of genes icaABCD by polymerase chain reaction. Biofilm production was observed in 96.67% of strains of S. aureus. These 43.34% were strong producers, 30.00% producing moderate, 23.33% weak producers and 3.33% non producing biofilm. All of the icaABCD genes was obtained at 6.66% of the isolates. All strains, except one, presented icaA or icaD. 56,66% of isolates identified genes icaA/icaD. The icaABCD operon genes were identified in all biofilm-producing strains, which demonstrates that they possess a virulence factor that facilitates its persistence in the breast tissue. In addition, the biofilm is a source of microbial contamination which can lead to failures in production processes that generate problems of food safety. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English Spanish |
topic |
Biofilm S. aureus genes ica leche cruda Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Biofilm S. aureus genes ica leche cruda Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Messaria Ginestre P. Yeiny Ávila R. Kutchynskaya Valero L. Jhoandry Rivera S. Wilfrido Briñez Z. Robert Valeris C. Biofilm production and presence of genes icaABCD in Staphylococcus aureus strains isolated from raw milk |
topic_facet |
Biofilm S. aureus genes ica leche cruda Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
In the pathogenesis of staphylococcal mastitis the biofilm is considered a marker of virulence that allows the adhesion of the microorganism to the mammary epithelium. In order to determine the production of biofilm and the presence of icaABCD gene, 30 S. aureus strains isolated from raw milk obtained from cows with subclinical mastitis were analyzed. Biofilm production was determined by the quantitative method on microplates for cell culture and the presence of genes icaABCD by polymerase chain reaction. Biofilm production was observed in 96.67% of strains of S. aureus. These 43.34% were strong producers, 30.00% producing moderate, 23.33% weak producers and 3.33% non producing biofilm. All of the icaABCD genes was obtained at 6.66% of the isolates. All strains, except one, presented icaA or icaD. 56,66% of isolates identified genes icaA/icaD. The icaABCD operon genes were identified in all biofilm-producing strains, which demonstrates that they possess a virulence factor that facilitates its persistence in the breast tissue. In addition, the biofilm is a source of microbial contamination which can lead to failures in production processes that generate problems of food safety. |
format |
Article in Journal/Newspaper |
author |
Messaria Ginestre P. Yeiny Ávila R. Kutchynskaya Valero L. Jhoandry Rivera S. Wilfrido Briñez Z. Robert Valeris C. |
author_facet |
Messaria Ginestre P. Yeiny Ávila R. Kutchynskaya Valero L. Jhoandry Rivera S. Wilfrido Briñez Z. Robert Valeris C. |
author_sort |
Messaria Ginestre P. |
title |
Biofilm production and presence of genes icaABCD in Staphylococcus aureus strains isolated from raw milk |
title_short |
Biofilm production and presence of genes icaABCD in Staphylococcus aureus strains isolated from raw milk |
title_full |
Biofilm production and presence of genes icaABCD in Staphylococcus aureus strains isolated from raw milk |
title_fullStr |
Biofilm production and presence of genes icaABCD in Staphylococcus aureus strains isolated from raw milk |
title_full_unstemmed |
Biofilm production and presence of genes icaABCD in Staphylococcus aureus strains isolated from raw milk |
title_sort |
biofilm production and presence of genes icaabcd in staphylococcus aureus strains isolated from raw milk |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2017 |
url |
https://doaj.org/article/c64f009717ef484bb3474c3abda97299 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 45, Iss 2, Pp 79-87 (2017) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/23058 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/c64f009717ef484bb3474c3abda97299 |
_version_ |
1779312159905284096 |