Correcting for enzyme immunoassay changes in long term monitoring studies
Enzyme immunoassays (EIAs) are a common tool for measuring steroid hormones in wildlife due to their low cost, commercial availability, and rapid results. Testing technologies improve continuously, sometimes requiring changes in protocols or crucial assay components. Antibody replacement between EIA...
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ftdoajarticles:oai:doaj.org/article:c638c79785aa406398ea518954cd416b 2023-05-15T18:42:05+02:00 Correcting for enzyme immunoassay changes in long term monitoring studies Abbey E. Wilson Agnieszka Sergiel Nuria Selva Jon E. Swenson Andreas Zedrosser Gordon Stenhouse David M. Janz 2021-01-01T00:00:00Z https://doi.org/10.1016/j.mex.2021.101212 https://doaj.org/article/c638c79785aa406398ea518954cd416b EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2215016121000042 https://doaj.org/toc/2215-0161 2215-0161 doi:10.1016/j.mex.2021.101212 https://doaj.org/article/c638c79785aa406398ea518954cd416b MethodsX, Vol 8, Iss , Pp 101212- (2021) Correcting for enzyme immunoassay variation Science Q article 2021 ftdoajarticles https://doi.org/10.1016/j.mex.2021.101212 2022-12-31T15:49:00Z Enzyme immunoassays (EIAs) are a common tool for measuring steroid hormones in wildlife due to their low cost, commercial availability, and rapid results. Testing technologies improve continuously, sometimes requiring changes in protocols or crucial assay components. Antibody replacement between EIA kits can cause differences in EIA sensitivity, which can hinder monitoring hormone concentration over time. The antibody in a common cortisol EIA kit used for long-term monitoring of stress in wildlife was replaced in 2014, causing differences in cross reactivity and standard curve concentrations. Therefore, the objective of this study was to develop a method to standardize results following changes in EIA sensitivity. We validated this method using cortisol concentrations measured in the hair of brown bears (Ursus arctos). • We used a simple linear regression to model the relationship between cortisol concentrations using kit 1 and kit 2. • We found a linear relationship between the two kits (R2 = 0.85) and used the regression equation (kit2 = (0.98 × kit1) + 1.65) to predict cortisol concentrations in re-measured samples. • Mean predicted percent error was 16% and 72% of samples had a predicted percent error <20%, suggesting that this method is well-suited for correcting changes in EIA sensitivity. Article in Journal/Newspaper Ursus arctos Directory of Open Access Journals: DOAJ Articles Eia ENVELOPE(7.755,7.755,63.024,63.024) MethodsX 8 101212 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Correcting for enzyme immunoassay variation Science Q |
spellingShingle |
Correcting for enzyme immunoassay variation Science Q Abbey E. Wilson Agnieszka Sergiel Nuria Selva Jon E. Swenson Andreas Zedrosser Gordon Stenhouse David M. Janz Correcting for enzyme immunoassay changes in long term monitoring studies |
topic_facet |
Correcting for enzyme immunoassay variation Science Q |
description |
Enzyme immunoassays (EIAs) are a common tool for measuring steroid hormones in wildlife due to their low cost, commercial availability, and rapid results. Testing technologies improve continuously, sometimes requiring changes in protocols or crucial assay components. Antibody replacement between EIA kits can cause differences in EIA sensitivity, which can hinder monitoring hormone concentration over time. The antibody in a common cortisol EIA kit used for long-term monitoring of stress in wildlife was replaced in 2014, causing differences in cross reactivity and standard curve concentrations. Therefore, the objective of this study was to develop a method to standardize results following changes in EIA sensitivity. We validated this method using cortisol concentrations measured in the hair of brown bears (Ursus arctos). • We used a simple linear regression to model the relationship between cortisol concentrations using kit 1 and kit 2. • We found a linear relationship between the two kits (R2 = 0.85) and used the regression equation (kit2 = (0.98 × kit1) + 1.65) to predict cortisol concentrations in re-measured samples. • Mean predicted percent error was 16% and 72% of samples had a predicted percent error <20%, suggesting that this method is well-suited for correcting changes in EIA sensitivity. |
format |
Article in Journal/Newspaper |
author |
Abbey E. Wilson Agnieszka Sergiel Nuria Selva Jon E. Swenson Andreas Zedrosser Gordon Stenhouse David M. Janz |
author_facet |
Abbey E. Wilson Agnieszka Sergiel Nuria Selva Jon E. Swenson Andreas Zedrosser Gordon Stenhouse David M. Janz |
author_sort |
Abbey E. Wilson |
title |
Correcting for enzyme immunoassay changes in long term monitoring studies |
title_short |
Correcting for enzyme immunoassay changes in long term monitoring studies |
title_full |
Correcting for enzyme immunoassay changes in long term monitoring studies |
title_fullStr |
Correcting for enzyme immunoassay changes in long term monitoring studies |
title_full_unstemmed |
Correcting for enzyme immunoassay changes in long term monitoring studies |
title_sort |
correcting for enzyme immunoassay changes in long term monitoring studies |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doi.org/10.1016/j.mex.2021.101212 https://doaj.org/article/c638c79785aa406398ea518954cd416b |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Eia |
geographic_facet |
Eia |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
MethodsX, Vol 8, Iss , Pp 101212- (2021) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2215016121000042 https://doaj.org/toc/2215-0161 2215-0161 doi:10.1016/j.mex.2021.101212 https://doaj.org/article/c638c79785aa406398ea518954cd416b |
op_doi |
https://doi.org/10.1016/j.mex.2021.101212 |
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MethodsX |
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8 |
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101212 |
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