Innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients.
Chronic chagasic cardiomyopathy (CCC) is observed in 30% to 50% of the individuals infected by Trypanosoma cruzi and heart failure is the important cause of death among patients in the chronic phase of Chagas disease. Although some studies have elucidated the role of adaptive immune responses involv...
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ftdoajarticles:oai:doaj.org/article:c6374711bb52406b912796e0cca374e7 2023-05-15T15:16:35+02:00 Innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients. Nathalie de Sena Pereira Tamyres Bernadete Dantas Queiroga Daniela Ferreira Nunes Cléber de Mesquita Andrade Manuela Sales Lima Nascimento Maria Adelaide Do-Valle-Matta Antônia Cláudia Jácome da Câmara Lúcia Maria da Cunha Galvão Paulo Marcos Matta Guedes Egler Chiari 2018-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006589 https://doaj.org/article/c6374711bb52406b912796e0cca374e7 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6078325?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006589 https://doaj.org/article/c6374711bb52406b912796e0cca374e7 PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006589 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006589 2022-12-31T08:17:05Z Chronic chagasic cardiomyopathy (CCC) is observed in 30% to 50% of the individuals infected by Trypanosoma cruzi and heart failure is the important cause of death among patients in the chronic phase of Chagas disease. Although some studies have elucidated the role of adaptive immune responses involving T and B lymphocytes in cardiac pathogenesis, the role of innate immunity receptors such as Toll-like receptors (TLRs) and Nod-like receptors (NLRs) in CCC pathophysiology has not yet been determined. In this study, we evaluated the association among innate immune receptors (TLR1-9 and nucleotide-binding domain-like receptor protein 3/NLRP3), its adapter molecules (Myd88, TRIF, ASC and caspase-1) and cytokines (IL-1β, IL-6, IL-12, IL-18, IL-23, TNF-α, and IFN-β) with clinical manifestation, digestive and cardiac function in patients with different clinical forms of chronic Chagas disease. The TLR8 mRNA expression levels were enhanced in the peripheral blood mononuclear cells (PBMC) from digestive and cardiodigestive patients compared to indeterminate and cardiac patients. Furthermore, mRNA expression of IFN-β (cytokine produced after TLR8 activation) was higher in digestive and cardiodigestive patients when compared to indeterminate. Moreover, there was a positive correlation between TLR8 and IFN-β mRNA expression with sigmoid and rectum size. Cardiac and cardiodigestive patients presented higher TLR2, IL-12 and TNF-α mRNA expression than indeterminate and digestive patients. Moreover, cardiac patients also expressed higher levels of NLRP3, ASC and IL-1β mRNAs than indeterminate patients. In addition, we showed a negative correlation among TLR2, IL-1β, IL-12 and TNF-α levels with left ventricular ejection fraction, and positive correlation between NLRP3 with cardiothoracic index, and TLR2, IL-1β and IL-12 with left ventricular mass index. Together, our data suggest that high expression of innate immune receptors in cardiac and digestive patients may induce an enhancement of cytokine expression and participate of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 7 e0006589 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Nathalie de Sena Pereira Tamyres Bernadete Dantas Queiroga Daniela Ferreira Nunes Cléber de Mesquita Andrade Manuela Sales Lima Nascimento Maria Adelaide Do-Valle-Matta Antônia Cláudia Jácome da Câmara Lúcia Maria da Cunha Galvão Paulo Marcos Matta Guedes Egler Chiari Innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chronic chagasic cardiomyopathy (CCC) is observed in 30% to 50% of the individuals infected by Trypanosoma cruzi and heart failure is the important cause of death among patients in the chronic phase of Chagas disease. Although some studies have elucidated the role of adaptive immune responses involving T and B lymphocytes in cardiac pathogenesis, the role of innate immunity receptors such as Toll-like receptors (TLRs) and Nod-like receptors (NLRs) in CCC pathophysiology has not yet been determined. In this study, we evaluated the association among innate immune receptors (TLR1-9 and nucleotide-binding domain-like receptor protein 3/NLRP3), its adapter molecules (Myd88, TRIF, ASC and caspase-1) and cytokines (IL-1β, IL-6, IL-12, IL-18, IL-23, TNF-α, and IFN-β) with clinical manifestation, digestive and cardiac function in patients with different clinical forms of chronic Chagas disease. The TLR8 mRNA expression levels were enhanced in the peripheral blood mononuclear cells (PBMC) from digestive and cardiodigestive patients compared to indeterminate and cardiac patients. Furthermore, mRNA expression of IFN-β (cytokine produced after TLR8 activation) was higher in digestive and cardiodigestive patients when compared to indeterminate. Moreover, there was a positive correlation between TLR8 and IFN-β mRNA expression with sigmoid and rectum size. Cardiac and cardiodigestive patients presented higher TLR2, IL-12 and TNF-α mRNA expression than indeterminate and digestive patients. Moreover, cardiac patients also expressed higher levels of NLRP3, ASC and IL-1β mRNAs than indeterminate patients. In addition, we showed a negative correlation among TLR2, IL-1β, IL-12 and TNF-α levels with left ventricular ejection fraction, and positive correlation between NLRP3 with cardiothoracic index, and TLR2, IL-1β and IL-12 with left ventricular mass index. Together, our data suggest that high expression of innate immune receptors in cardiac and digestive patients may induce an enhancement of cytokine expression and participate of ... |
format |
Article in Journal/Newspaper |
author |
Nathalie de Sena Pereira Tamyres Bernadete Dantas Queiroga Daniela Ferreira Nunes Cléber de Mesquita Andrade Manuela Sales Lima Nascimento Maria Adelaide Do-Valle-Matta Antônia Cláudia Jácome da Câmara Lúcia Maria da Cunha Galvão Paulo Marcos Matta Guedes Egler Chiari |
author_facet |
Nathalie de Sena Pereira Tamyres Bernadete Dantas Queiroga Daniela Ferreira Nunes Cléber de Mesquita Andrade Manuela Sales Lima Nascimento Maria Adelaide Do-Valle-Matta Antônia Cláudia Jácome da Câmara Lúcia Maria da Cunha Galvão Paulo Marcos Matta Guedes Egler Chiari |
author_sort |
Nathalie de Sena Pereira |
title |
Innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients. |
title_short |
Innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients. |
title_full |
Innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients. |
title_fullStr |
Innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients. |
title_full_unstemmed |
Innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients. |
title_sort |
innate immune receptors over expression correlate with chronic chagasic cardiomyopathy and digestive damage in patients. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006589 https://doaj.org/article/c6374711bb52406b912796e0cca374e7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006589 (2018) |
op_relation |
http://europepmc.org/articles/PMC6078325?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006589 https://doaj.org/article/c6374711bb52406b912796e0cca374e7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006589 |
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PLOS Neglected Tropical Diseases |
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12 |
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7 |
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e0006589 |
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