The PortaLyzer, a DIY tool that allows environmental DNA extraction in the field
The PortaLyzer is a portable homemade device that allows researchers to perform bead-beating steps commonly found in environmental DNA (eDNA) extraction protocols in the field without access to power. This allows researchers to preserve in situ organism abundance by beginning eDNA extraction quickly...
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2022
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Online Access: | https://doi.org/10.1016/j.ohx.2022.e00373 https://doaj.org/article/c6314e75efb04af88577883191560a09 |
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ftdoajarticles:oai:doaj.org/article:c6314e75efb04af88577883191560a09 2023-05-15T16:50:06+02:00 The PortaLyzer, a DIY tool that allows environmental DNA extraction in the field Charlie Peck Faith Jackobs Emmett Smith 2022-10-01T00:00:00Z https://doi.org/10.1016/j.ohx.2022.e00373 https://doaj.org/article/c6314e75efb04af88577883191560a09 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2468067222001183 https://doaj.org/toc/2468-0672 2468-0672 doi:10.1016/j.ohx.2022.e00373 https://doaj.org/article/c6314e75efb04af88577883191560a09 HardwareX, Vol 12, Iss , Pp e00373- (2022) Environmental DNA Field DNA extraction Bead-beating Portable Science (General) Q1-390 article 2022 ftdoajarticles https://doi.org/10.1016/j.ohx.2022.e00373 2022-12-30T20:58:26Z The PortaLyzer is a portable homemade device that allows researchers to perform bead-beating steps commonly found in environmental DNA (eDNA) extraction protocols in the field without access to power. This allows researchers to preserve in situ organism abundance by beginning eDNA extraction quickly. The PortaLyzer is composed of a variable speed, battery-powered multi-tool and a vortexer adapter plate. We used the PortaLyzer, in conjunction with the Qiagen DNEasy PowerSoil Pro kit, to successfully field process samples taken from the forelands of the Sólheimajökull and Kvíárjökull glaciers in Iceland, as well as soil samples acquired from a prairie in Indiana. Additionally, we provide evidence that samples held in Buffer CD2 of the DNEasy PowerSoil Pro Kit may be transported to traditional lab spaces and processed up to one month after the initial protocol steps, and still provide an equivalent DNA quality and abundance yield as those processed the same day. These improvements to DNA extraction protocols give researchers more flexibility while sampling, shipping and processing eDNA samples. Article in Journal/Newspaper Iceland Directory of Open Access Journals: DOAJ Articles Sólheimajökull ENVELOPE(-19.303,-19.303,63.557,63.557) HardwareX 12 e00373 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Environmental DNA Field DNA extraction Bead-beating Portable Science (General) Q1-390 |
spellingShingle |
Environmental DNA Field DNA extraction Bead-beating Portable Science (General) Q1-390 Charlie Peck Faith Jackobs Emmett Smith The PortaLyzer, a DIY tool that allows environmental DNA extraction in the field |
topic_facet |
Environmental DNA Field DNA extraction Bead-beating Portable Science (General) Q1-390 |
description |
The PortaLyzer is a portable homemade device that allows researchers to perform bead-beating steps commonly found in environmental DNA (eDNA) extraction protocols in the field without access to power. This allows researchers to preserve in situ organism abundance by beginning eDNA extraction quickly. The PortaLyzer is composed of a variable speed, battery-powered multi-tool and a vortexer adapter plate. We used the PortaLyzer, in conjunction with the Qiagen DNEasy PowerSoil Pro kit, to successfully field process samples taken from the forelands of the Sólheimajökull and Kvíárjökull glaciers in Iceland, as well as soil samples acquired from a prairie in Indiana. Additionally, we provide evidence that samples held in Buffer CD2 of the DNEasy PowerSoil Pro Kit may be transported to traditional lab spaces and processed up to one month after the initial protocol steps, and still provide an equivalent DNA quality and abundance yield as those processed the same day. These improvements to DNA extraction protocols give researchers more flexibility while sampling, shipping and processing eDNA samples. |
format |
Article in Journal/Newspaper |
author |
Charlie Peck Faith Jackobs Emmett Smith |
author_facet |
Charlie Peck Faith Jackobs Emmett Smith |
author_sort |
Charlie Peck |
title |
The PortaLyzer, a DIY tool that allows environmental DNA extraction in the field |
title_short |
The PortaLyzer, a DIY tool that allows environmental DNA extraction in the field |
title_full |
The PortaLyzer, a DIY tool that allows environmental DNA extraction in the field |
title_fullStr |
The PortaLyzer, a DIY tool that allows environmental DNA extraction in the field |
title_full_unstemmed |
The PortaLyzer, a DIY tool that allows environmental DNA extraction in the field |
title_sort |
portalyzer, a diy tool that allows environmental dna extraction in the field |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.ohx.2022.e00373 https://doaj.org/article/c6314e75efb04af88577883191560a09 |
long_lat |
ENVELOPE(-19.303,-19.303,63.557,63.557) |
geographic |
Sólheimajökull |
geographic_facet |
Sólheimajökull |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
HardwareX, Vol 12, Iss , Pp e00373- (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2468067222001183 https://doaj.org/toc/2468-0672 2468-0672 doi:10.1016/j.ohx.2022.e00373 https://doaj.org/article/c6314e75efb04af88577883191560a09 |
op_doi |
https://doi.org/10.1016/j.ohx.2022.e00373 |
container_title |
HardwareX |
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12 |
container_start_page |
e00373 |
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1766040285077307392 |