Sensitivity of polymerase chain reaction for detection of known aliquots of Trypanosoma cruzi in the blood of mice: an in vitro study
To evaluate the sensitivity of polymerase chain reaction (PCR) to reveal known number of trypomastigote in the blood of mice, three separate experiments were done. First: To eight samples of 500mul of normal mice blood, one aliquot of 1, 2, 3, 4, 5, 10, and 50 trypomastigotes respectively, were adde...
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Sociedade Brasileira de Medicina Tropical (SBMT)
2002
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ftdoajarticles:oai:doaj.org/article:c5ee264030ad4455b9818323827ed013 2023-05-15T15:08:44+02:00 Sensitivity of polymerase chain reaction for detection of known aliquots of Trypanosoma cruzi in the blood of mice: an in vitro study Rozália F. Campos Juracy B. Magalhães Eliana A.G. Reis Mitermayer G. Reis Sonia G. Andrade 2002-10-01T00:00:00Z https://doaj.org/article/c5ee264030ad4455b9818323827ed013 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822002000500011&tlng=en https://doaj.org/toc/1678-9849 1678-9849 https://doaj.org/article/c5ee264030ad4455b9818323827ed013 Revista da Sociedade Brasileira de Medicina Tropical, Vol 35, Iss 5, Pp 487-490 (2002) Trypanosoma cruzi Polymerase chain reaction PCR sensitivity in mice PCR as cure test in mice Arctic medicine. Tropical medicine RC955-962 article 2002 ftdoajarticles 2022-12-30T21:38:58Z To evaluate the sensitivity of polymerase chain reaction (PCR) to reveal known number of trypomastigote in the blood of mice, three separate experiments were done. First: To eight samples of 500mul of normal mice blood, one aliquot of 1, 2, 3, 4, 5, 10, and 50 trypomastigotes respectively, were added. Second and third: 10 aliquots with 1 and 10 with 2 trypomastigotes were added to samples of 500mul of normal mice blood. Positive control: 500mul of blood containing 100,000 trypomastigotes. For kDNA minicircles amplification by PCR the primers:S35 and S36 were used. PCR revealed products of 330 b.p in the positive controls. When only one sample with the aliquots of 1 or 2 trypomastigotes was examined, results were negative; results were positive with aliquots of 3 to 50 trypomastigotes. In the 2nd and 3rd experiments, 9/10 aliquots with one parasite and 9/10 with 2 trypomastigotes were positive revealing a high sensitivity of this reaction. In conclusion, the presence of one single parasite in 500mul of blood, is enough for a positive PCR. This method could be used as a complement to the various parasitological cure tests in treated mice, when low volumes of blood are individually examined. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Trypanosoma cruzi Polymerase chain reaction PCR sensitivity in mice PCR as cure test in mice Arctic medicine. Tropical medicine RC955-962 |
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Trypanosoma cruzi Polymerase chain reaction PCR sensitivity in mice PCR as cure test in mice Arctic medicine. Tropical medicine RC955-962 Rozália F. Campos Juracy B. Magalhães Eliana A.G. Reis Mitermayer G. Reis Sonia G. Andrade Sensitivity of polymerase chain reaction for detection of known aliquots of Trypanosoma cruzi in the blood of mice: an in vitro study |
topic_facet |
Trypanosoma cruzi Polymerase chain reaction PCR sensitivity in mice PCR as cure test in mice Arctic medicine. Tropical medicine RC955-962 |
description |
To evaluate the sensitivity of polymerase chain reaction (PCR) to reveal known number of trypomastigote in the blood of mice, three separate experiments were done. First: To eight samples of 500mul of normal mice blood, one aliquot of 1, 2, 3, 4, 5, 10, and 50 trypomastigotes respectively, were added. Second and third: 10 aliquots with 1 and 10 with 2 trypomastigotes were added to samples of 500mul of normal mice blood. Positive control: 500mul of blood containing 100,000 trypomastigotes. For kDNA minicircles amplification by PCR the primers:S35 and S36 were used. PCR revealed products of 330 b.p in the positive controls. When only one sample with the aliquots of 1 or 2 trypomastigotes was examined, results were negative; results were positive with aliquots of 3 to 50 trypomastigotes. In the 2nd and 3rd experiments, 9/10 aliquots with one parasite and 9/10 with 2 trypomastigotes were positive revealing a high sensitivity of this reaction. In conclusion, the presence of one single parasite in 500mul of blood, is enough for a positive PCR. This method could be used as a complement to the various parasitological cure tests in treated mice, when low volumes of blood are individually examined. |
format |
Article in Journal/Newspaper |
author |
Rozália F. Campos Juracy B. Magalhães Eliana A.G. Reis Mitermayer G. Reis Sonia G. Andrade |
author_facet |
Rozália F. Campos Juracy B. Magalhães Eliana A.G. Reis Mitermayer G. Reis Sonia G. Andrade |
author_sort |
Rozália F. Campos |
title |
Sensitivity of polymerase chain reaction for detection of known aliquots of Trypanosoma cruzi in the blood of mice: an in vitro study |
title_short |
Sensitivity of polymerase chain reaction for detection of known aliquots of Trypanosoma cruzi in the blood of mice: an in vitro study |
title_full |
Sensitivity of polymerase chain reaction for detection of known aliquots of Trypanosoma cruzi in the blood of mice: an in vitro study |
title_fullStr |
Sensitivity of polymerase chain reaction for detection of known aliquots of Trypanosoma cruzi in the blood of mice: an in vitro study |
title_full_unstemmed |
Sensitivity of polymerase chain reaction for detection of known aliquots of Trypanosoma cruzi in the blood of mice: an in vitro study |
title_sort |
sensitivity of polymerase chain reaction for detection of known aliquots of trypanosoma cruzi in the blood of mice: an in vitro study |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2002 |
url |
https://doaj.org/article/c5ee264030ad4455b9818323827ed013 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 35, Iss 5, Pp 487-490 (2002) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822002000500011&tlng=en https://doaj.org/toc/1678-9849 1678-9849 https://doaj.org/article/c5ee264030ad4455b9818323827ed013 |
_version_ |
1766340043080728576 |