Hydrophilic Shell Matrix Proteins of Nautilus pompilius and the Identification of a Core Set of Conchiferan Domains
Despite being a member of the shelled mollusks (Conchiferans), most members of extant cephalopods have lost their external biomineralized shells, except for the basally diverging Nautilids. Here, we report the result of our study to identify major Shell Matrix Proteins and their domains in the Nauti...
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Language: | English |
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MDPI AG
2021
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Online Access: | https://doi.org/10.3390/genes12121925 https://doaj.org/article/c5cca66cae1b4db9944d3d031c3903d1 |
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ftdoajarticles:oai:doaj.org/article:c5cca66cae1b4db9944d3d031c3903d1 2023-05-15T17:54:20+02:00 Hydrophilic Shell Matrix Proteins of Nautilus pompilius and the Identification of a Core Set of Conchiferan Domains Davin H. E. Setiamarga Kazuki Hirota Masa-aki Yoshida Yusuke Takeda Keiji Kito Makiko Ishikawa Keisuke Shimizu Yukinobu Isowa Kazuho Ikeo Takenori Sasaki Kazuyoshi Endo 2021-11-01T00:00:00Z https://doi.org/10.3390/genes12121925 https://doaj.org/article/c5cca66cae1b4db9944d3d031c3903d1 EN eng MDPI AG https://www.mdpi.com/2073-4425/12/12/1925 https://doaj.org/toc/2073-4425 doi:10.3390/genes12121925 2073-4425 https://doaj.org/article/c5cca66cae1b4db9944d3d031c3903d1 Genes, Vol 12, Iss 1925, p 1925 (2021) biomineralization multiomics proteomics shell evolution Mollusca Cephalopoda Genetics QH426-470 article 2021 ftdoajarticles https://doi.org/10.3390/genes12121925 2022-12-31T11:00:02Z Despite being a member of the shelled mollusks (Conchiferans), most members of extant cephalopods have lost their external biomineralized shells, except for the basally diverging Nautilids. Here, we report the result of our study to identify major Shell Matrix Proteins and their domains in the Nautilid Nautilus pompilius, in order to gain a general insight into the evolution of Conchiferan Shell Matrix Proteins. In order to do so, we performed a multiomics study on the shell of N. pompilius , by conducting transcriptomics of its mantle tissue and proteomics of its shell matrix. Analyses of obtained data identified 61 distinct shell-specific sequences. Of the successfully annotated 27 sequences, protein domains were predicted in 19. Comparative analysis of Nautilus sequences with four Conchiferans for which Shell Matrix Protein data were available (the pacific oyster, the pearl oyster, the limpet and the Euhadra snail) revealed that three proteins and six protein domains were conserved in all Conchiferans. Interestingly, when the terrestrial Euhadra snail was excluded, another five proteins and six protein domains were found to be shared among the four marine Conchiferans. Phylogenetic analyses indicated that most of these proteins and domains were probably present in the ancestral Conchiferan, but employed in shell formation later and independently in most clades. Even though further studies utilizing deeper sequencing techniques to obtain genome and full-length sequences, and functional analyses, must be carried out in the future, our results here provide important pieces of information for the elucidation of the evolution of Conchiferan shells at the molecular level. Article in Journal/Newspaper Pacific oyster Directory of Open Access Journals: DOAJ Articles Nautilus ENVELOPE(-67.167,-67.167,-67.650,-67.650) Pacific Genes 12 12 1925 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
biomineralization multiomics proteomics shell evolution Mollusca Cephalopoda Genetics QH426-470 |
spellingShingle |
biomineralization multiomics proteomics shell evolution Mollusca Cephalopoda Genetics QH426-470 Davin H. E. Setiamarga Kazuki Hirota Masa-aki Yoshida Yusuke Takeda Keiji Kito Makiko Ishikawa Keisuke Shimizu Yukinobu Isowa Kazuho Ikeo Takenori Sasaki Kazuyoshi Endo Hydrophilic Shell Matrix Proteins of Nautilus pompilius and the Identification of a Core Set of Conchiferan Domains |
topic_facet |
biomineralization multiomics proteomics shell evolution Mollusca Cephalopoda Genetics QH426-470 |
description |
Despite being a member of the shelled mollusks (Conchiferans), most members of extant cephalopods have lost their external biomineralized shells, except for the basally diverging Nautilids. Here, we report the result of our study to identify major Shell Matrix Proteins and their domains in the Nautilid Nautilus pompilius, in order to gain a general insight into the evolution of Conchiferan Shell Matrix Proteins. In order to do so, we performed a multiomics study on the shell of N. pompilius , by conducting transcriptomics of its mantle tissue and proteomics of its shell matrix. Analyses of obtained data identified 61 distinct shell-specific sequences. Of the successfully annotated 27 sequences, protein domains were predicted in 19. Comparative analysis of Nautilus sequences with four Conchiferans for which Shell Matrix Protein data were available (the pacific oyster, the pearl oyster, the limpet and the Euhadra snail) revealed that three proteins and six protein domains were conserved in all Conchiferans. Interestingly, when the terrestrial Euhadra snail was excluded, another five proteins and six protein domains were found to be shared among the four marine Conchiferans. Phylogenetic analyses indicated that most of these proteins and domains were probably present in the ancestral Conchiferan, but employed in shell formation later and independently in most clades. Even though further studies utilizing deeper sequencing techniques to obtain genome and full-length sequences, and functional analyses, must be carried out in the future, our results here provide important pieces of information for the elucidation of the evolution of Conchiferan shells at the molecular level. |
format |
Article in Journal/Newspaper |
author |
Davin H. E. Setiamarga Kazuki Hirota Masa-aki Yoshida Yusuke Takeda Keiji Kito Makiko Ishikawa Keisuke Shimizu Yukinobu Isowa Kazuho Ikeo Takenori Sasaki Kazuyoshi Endo |
author_facet |
Davin H. E. Setiamarga Kazuki Hirota Masa-aki Yoshida Yusuke Takeda Keiji Kito Makiko Ishikawa Keisuke Shimizu Yukinobu Isowa Kazuho Ikeo Takenori Sasaki Kazuyoshi Endo |
author_sort |
Davin H. E. Setiamarga |
title |
Hydrophilic Shell Matrix Proteins of Nautilus pompilius and the Identification of a Core Set of Conchiferan Domains |
title_short |
Hydrophilic Shell Matrix Proteins of Nautilus pompilius and the Identification of a Core Set of Conchiferan Domains |
title_full |
Hydrophilic Shell Matrix Proteins of Nautilus pompilius and the Identification of a Core Set of Conchiferan Domains |
title_fullStr |
Hydrophilic Shell Matrix Proteins of Nautilus pompilius and the Identification of a Core Set of Conchiferan Domains |
title_full_unstemmed |
Hydrophilic Shell Matrix Proteins of Nautilus pompilius and the Identification of a Core Set of Conchiferan Domains |
title_sort |
hydrophilic shell matrix proteins of nautilus pompilius and the identification of a core set of conchiferan domains |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/genes12121925 https://doaj.org/article/c5cca66cae1b4db9944d3d031c3903d1 |
long_lat |
ENVELOPE(-67.167,-67.167,-67.650,-67.650) |
geographic |
Nautilus Pacific |
geographic_facet |
Nautilus Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_source |
Genes, Vol 12, Iss 1925, p 1925 (2021) |
op_relation |
https://www.mdpi.com/2073-4425/12/12/1925 https://doaj.org/toc/2073-4425 doi:10.3390/genes12121925 2073-4425 https://doaj.org/article/c5cca66cae1b4db9944d3d031c3903d1 |
op_doi |
https://doi.org/10.3390/genes12121925 |
container_title |
Genes |
container_volume |
12 |
container_issue |
12 |
container_start_page |
1925 |
_version_ |
1766162091780079616 |