Neutrophil Extracellular Traps Identification in Tegumentary Lesions of Patients with Paracoccidioidomycosis and Different Patterns of NETs Generation In Vitro.
Paracoccidioidomycosis (PCM) is a systemic mycosis, endemic in most Latin American countries, especially in Brazil. It is caused by the thermo-dimorphic fungus of the genus Paracoccidioides (Paracoccidioides brasiliensis and Paracoccidioides lutzii). Innate immune response plays a crucial role in ho...
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ftdoajarticles:oai:doaj.org/article:c5bedc85336e479096c94af27dc5d900 2023-05-15T15:13:29+02:00 Neutrophil Extracellular Traps Identification in Tegumentary Lesions of Patients with Paracoccidioidomycosis and Different Patterns of NETs Generation In Vitro. Amanda Manoel Della Coletta Tatiana Fernanda Bachiega Juliana Carvalho de Quaglia e Silva Ângela Maria Victoriano de Campos Soares Julio De Faveri Silvio Alencar Marques Mariângela Esther Alencar Marques Valdecir Farias Ximenes Luciane Alarcão Dias-Melicio 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004037 https://doaj.org/article/c5bedc85336e479096c94af27dc5d900 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4556621?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004037 https://doaj.org/article/c5bedc85336e479096c94af27dc5d900 PLoS Neglected Tropical Diseases, Vol 9, Iss 9, p e0004037 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004037 2022-12-31T06:31:52Z Paracoccidioidomycosis (PCM) is a systemic mycosis, endemic in most Latin American countries, especially in Brazil. It is caused by the thermo-dimorphic fungus of the genus Paracoccidioides (Paracoccidioides brasiliensis and Paracoccidioides lutzii). Innate immune response plays a crucial role in host defense against fungal infections, and neutrophils (PMNs) are able to combat microorganisms with three different mechanisms: phagocytosis, secretion of granular proteins, which have antimicrobial properties, and the most recent described mechanism called NETosis. This new process is characterized by the release of net-like structures called Neutrophil Extracellular Traps (NETs), which is composed of nuclear (decondensed DNA and histones) and granular material such as elastase. Several microorganisms have the ability of inducing NETs formation, including gram-positive and gram-negative bacteria, viruses and some fungi. We proposed to identify NETs in tegumentary lesions of patients with PCM and to analyze the interaction between two strains of P. brasiliensis and human PMNs by NETs formation in vitro. In this context, the presence of NETs in vivo was evidenced in tegumentary lesions of patients with PCM by confocal spectrum analyzer. Furthermore, we showed that the high virulent P. brasiliensis strain 18 (Pb18) and the lower virulent strain Pb265 are able to induce different patterns of NETs formation in vitro. The quantification of extracellular DNA corroborates the idea of the ability of P. brasiliensis in inducing NETs release. In conclusion, our data show for the first time the identification of NETs in lesions of patients with PCM and demonstrate distinct patterns of NETs in cultures challenged with fungi in vitro. The presence of NETs components both in vivo and in vitro open new possibilities for the detailed investigation of immunity in PCM. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 9 e0004037 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Amanda Manoel Della Coletta Tatiana Fernanda Bachiega Juliana Carvalho de Quaglia e Silva Ângela Maria Victoriano de Campos Soares Julio De Faveri Silvio Alencar Marques Mariângela Esther Alencar Marques Valdecir Farias Ximenes Luciane Alarcão Dias-Melicio Neutrophil Extracellular Traps Identification in Tegumentary Lesions of Patients with Paracoccidioidomycosis and Different Patterns of NETs Generation In Vitro. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Paracoccidioidomycosis (PCM) is a systemic mycosis, endemic in most Latin American countries, especially in Brazil. It is caused by the thermo-dimorphic fungus of the genus Paracoccidioides (Paracoccidioides brasiliensis and Paracoccidioides lutzii). Innate immune response plays a crucial role in host defense against fungal infections, and neutrophils (PMNs) are able to combat microorganisms with three different mechanisms: phagocytosis, secretion of granular proteins, which have antimicrobial properties, and the most recent described mechanism called NETosis. This new process is characterized by the release of net-like structures called Neutrophil Extracellular Traps (NETs), which is composed of nuclear (decondensed DNA and histones) and granular material such as elastase. Several microorganisms have the ability of inducing NETs formation, including gram-positive and gram-negative bacteria, viruses and some fungi. We proposed to identify NETs in tegumentary lesions of patients with PCM and to analyze the interaction between two strains of P. brasiliensis and human PMNs by NETs formation in vitro. In this context, the presence of NETs in vivo was evidenced in tegumentary lesions of patients with PCM by confocal spectrum analyzer. Furthermore, we showed that the high virulent P. brasiliensis strain 18 (Pb18) and the lower virulent strain Pb265 are able to induce different patterns of NETs formation in vitro. The quantification of extracellular DNA corroborates the idea of the ability of P. brasiliensis in inducing NETs release. In conclusion, our data show for the first time the identification of NETs in lesions of patients with PCM and demonstrate distinct patterns of NETs in cultures challenged with fungi in vitro. The presence of NETs components both in vivo and in vitro open new possibilities for the detailed investigation of immunity in PCM. |
format |
Article in Journal/Newspaper |
author |
Amanda Manoel Della Coletta Tatiana Fernanda Bachiega Juliana Carvalho de Quaglia e Silva Ângela Maria Victoriano de Campos Soares Julio De Faveri Silvio Alencar Marques Mariângela Esther Alencar Marques Valdecir Farias Ximenes Luciane Alarcão Dias-Melicio |
author_facet |
Amanda Manoel Della Coletta Tatiana Fernanda Bachiega Juliana Carvalho de Quaglia e Silva Ângela Maria Victoriano de Campos Soares Julio De Faveri Silvio Alencar Marques Mariângela Esther Alencar Marques Valdecir Farias Ximenes Luciane Alarcão Dias-Melicio |
author_sort |
Amanda Manoel Della Coletta |
title |
Neutrophil Extracellular Traps Identification in Tegumentary Lesions of Patients with Paracoccidioidomycosis and Different Patterns of NETs Generation In Vitro. |
title_short |
Neutrophil Extracellular Traps Identification in Tegumentary Lesions of Patients with Paracoccidioidomycosis and Different Patterns of NETs Generation In Vitro. |
title_full |
Neutrophil Extracellular Traps Identification in Tegumentary Lesions of Patients with Paracoccidioidomycosis and Different Patterns of NETs Generation In Vitro. |
title_fullStr |
Neutrophil Extracellular Traps Identification in Tegumentary Lesions of Patients with Paracoccidioidomycosis and Different Patterns of NETs Generation In Vitro. |
title_full_unstemmed |
Neutrophil Extracellular Traps Identification in Tegumentary Lesions of Patients with Paracoccidioidomycosis and Different Patterns of NETs Generation In Vitro. |
title_sort |
neutrophil extracellular traps identification in tegumentary lesions of patients with paracoccidioidomycosis and different patterns of nets generation in vitro. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0004037 https://doaj.org/article/c5bedc85336e479096c94af27dc5d900 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 9, p e0004037 (2015) |
op_relation |
http://europepmc.org/articles/PMC4556621?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004037 https://doaj.org/article/c5bedc85336e479096c94af27dc5d900 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004037 |
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PLOS Neglected Tropical Diseases |
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9 |
container_issue |
9 |
container_start_page |
e0004037 |
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