Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions
Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural subs...
Published in: | Frontiers in Chemistry |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fchem.2020.613388 https://doaj.org/article/c54d50fbd52947128fff02c91c948849 |
id |
ftdoajarticles:oai:doaj.org/article:c54d50fbd52947128fff02c91c948849 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:c54d50fbd52947128fff02c91c948849 2023-05-15T13:59:09+02:00 Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions Sandra Engelskirchen Stefan Wellert Olaf Holderer Henrich Frielinghaus Michaela Laupheimer Sven Richter Bettina Nestl Bernd Nebel Bernhard Hauer 2021-01-01T00:00:00Z https://doi.org/10.3389/fchem.2020.613388 https://doaj.org/article/c54d50fbd52947128fff02c91c948849 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fchem.2020.613388/full https://doaj.org/toc/2296-2646 2296-2646 doi:10.3389/fchem.2020.613388 https://doaj.org/article/c54d50fbd52947128fff02c91c948849 Frontiers in Chemistry, Vol 8 (2021) microemulsion lipase bending elasticity neutron scattering neutron spin echo Chemistry QD1-999 article 2021 ftdoajarticles https://doi.org/10.3389/fchem.2020.613388 2022-12-31T11:01:23Z Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water–oil interface. In the present paper, we exploit the fact that the presence of lipases, in particular the lipase from Candida antarctica B (CalB), changes the bending elastic properties of a surfactant monolayer in a bicontinuous microemulsion consisting of D2O/NaCl -n-(d)-octane-pentaethylene glycol monodecyl ether (C10E5) in a similar manner as previously observed for amphiphilic block-copolymers. To determine the bending elastic constant, we have used two approaches, small angle neutron scattering (SANS) and neutron spin echo (NSE) spectroscopy. The time-averaged structure from SANS showed a slight decrease in bending elasticity, while on nanosecond time scales as probed with NSE, a stiffening has been observed, which was attributed to adsorption/desorption mechanisms of CalB at the surfactant monolayer. The results allow to derive further information on the influence of CalB on the composition and bending elasticity of the surfactant monolayer itself as well as the underlying adsorption/desorption mechanism. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Frontiers in Chemistry 8 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
microemulsion lipase bending elasticity neutron scattering neutron spin echo Chemistry QD1-999 |
spellingShingle |
microemulsion lipase bending elasticity neutron scattering neutron spin echo Chemistry QD1-999 Sandra Engelskirchen Stefan Wellert Olaf Holderer Henrich Frielinghaus Michaela Laupheimer Sven Richter Bettina Nestl Bernd Nebel Bernhard Hauer Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
topic_facet |
microemulsion lipase bending elasticity neutron scattering neutron spin echo Chemistry QD1-999 |
description |
Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water–oil interface. In the present paper, we exploit the fact that the presence of lipases, in particular the lipase from Candida antarctica B (CalB), changes the bending elastic properties of a surfactant monolayer in a bicontinuous microemulsion consisting of D2O/NaCl -n-(d)-octane-pentaethylene glycol monodecyl ether (C10E5) in a similar manner as previously observed for amphiphilic block-copolymers. To determine the bending elastic constant, we have used two approaches, small angle neutron scattering (SANS) and neutron spin echo (NSE) spectroscopy. The time-averaged structure from SANS showed a slight decrease in bending elasticity, while on nanosecond time scales as probed with NSE, a stiffening has been observed, which was attributed to adsorption/desorption mechanisms of CalB at the surfactant monolayer. The results allow to derive further information on the influence of CalB on the composition and bending elasticity of the surfactant monolayer itself as well as the underlying adsorption/desorption mechanism. |
format |
Article in Journal/Newspaper |
author |
Sandra Engelskirchen Stefan Wellert Olaf Holderer Henrich Frielinghaus Michaela Laupheimer Sven Richter Bettina Nestl Bernd Nebel Bernhard Hauer |
author_facet |
Sandra Engelskirchen Stefan Wellert Olaf Holderer Henrich Frielinghaus Michaela Laupheimer Sven Richter Bettina Nestl Bernd Nebel Bernhard Hauer |
author_sort |
Sandra Engelskirchen |
title |
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
title_short |
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
title_full |
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
title_fullStr |
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
title_full_unstemmed |
Surfactant Monolayer Bending Elasticity in Lipase Containing Bicontinuous Microemulsions |
title_sort |
surfactant monolayer bending elasticity in lipase containing bicontinuous microemulsions |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fchem.2020.613388 https://doaj.org/article/c54d50fbd52947128fff02c91c948849 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Frontiers in Chemistry, Vol 8 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fchem.2020.613388/full https://doaj.org/toc/2296-2646 2296-2646 doi:10.3389/fchem.2020.613388 https://doaj.org/article/c54d50fbd52947128fff02c91c948849 |
op_doi |
https://doi.org/10.3389/fchem.2020.613388 |
container_title |
Frontiers in Chemistry |
container_volume |
8 |
_version_ |
1766267604348960768 |