The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat.
Extremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophil...
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ftdoajarticles:oai:doaj.org/article:c535eb7e07ea42f887282ca670758a77 2023-05-15T13:36:00+02:00 The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. Mohd Firdaus-Raih Noor Haza Fazlin Hashim Izwan Bharudin Mohd Faizal Abu Bakar Kie Kyon Huang Halimah Alias Bernard K B Lee Mohd Noor Mat Isa Shuhaila Mat-Sharani Suhaila Sulaiman Lih Jinq Tay Radziah Zolkefli Yusuf Muhammad Noor Douglas Sie Nguong Law Siti Hamidah Abdul Rahman Rosli Md-Illias Farah Diba Abu Bakar Nazalan Najimudin Abdul Munir Abdul Murad Nor Muhammad Mahadi 2018-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0189947 https://doaj.org/article/c535eb7e07ea42f887282ca670758a77 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5791967?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0189947 https://doaj.org/article/c535eb7e07ea42f887282ca670758a77 PLoS ONE, Vol 13, Iss 1, p e0189947 (2018) Medicine R Science Q article 2018 ftdoajarticles https://doi.org/10.1371/journal.pone.0189947 2022-12-31T02:14:59Z Extremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophilic yeast isolated in the Antarctic region, Glaciozyma antarctica. The genome annotation identified 7857 protein coding sequences. From the genome sequence analysis we were able to identify genes that encoded for proteins known to be associated with cold survival, in addition to annotating genes that are unique to G. antarctica. For genes that are known to be involved in cold adaptation such as anti-freeze proteins (AFPs), our gene expression analysis revealed that they were differentially transcribed over time and in response to different temperatures. This indicated the presence of an array of adaptation systems that can respond to a changing but persistent cold environment. We were also able to validate the activity of all the AFPs annotated where the recombinant AFPs demonstrated anti-freeze capacity. This work is an important foundation for further collective exploration into psychrophilic microbiology where among other potential, the genes unique to this species may represent a pool of novel mechanisms for cold survival. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic PLOS ONE 13 1 e0189947 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Mohd Firdaus-Raih Noor Haza Fazlin Hashim Izwan Bharudin Mohd Faizal Abu Bakar Kie Kyon Huang Halimah Alias Bernard K B Lee Mohd Noor Mat Isa Shuhaila Mat-Sharani Suhaila Sulaiman Lih Jinq Tay Radziah Zolkefli Yusuf Muhammad Noor Douglas Sie Nguong Law Siti Hamidah Abdul Rahman Rosli Md-Illias Farah Diba Abu Bakar Nazalan Najimudin Abdul Munir Abdul Murad Nor Muhammad Mahadi The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
topic_facet |
Medicine R Science Q |
description |
Extremely low temperatures present various challenges to life that include ice formation and effects on metabolic capacity. Psyhcrophilic microorganisms typically have an array of mechanisms to enable survival in cold temperatures. In this study, we sequenced and analysed the genome of a psychrophilic yeast isolated in the Antarctic region, Glaciozyma antarctica. The genome annotation identified 7857 protein coding sequences. From the genome sequence analysis we were able to identify genes that encoded for proteins known to be associated with cold survival, in addition to annotating genes that are unique to G. antarctica. For genes that are known to be involved in cold adaptation such as anti-freeze proteins (AFPs), our gene expression analysis revealed that they were differentially transcribed over time and in response to different temperatures. This indicated the presence of an array of adaptation systems that can respond to a changing but persistent cold environment. We were also able to validate the activity of all the AFPs annotated where the recombinant AFPs demonstrated anti-freeze capacity. This work is an important foundation for further collective exploration into psychrophilic microbiology where among other potential, the genes unique to this species may represent a pool of novel mechanisms for cold survival. |
format |
Article in Journal/Newspaper |
author |
Mohd Firdaus-Raih Noor Haza Fazlin Hashim Izwan Bharudin Mohd Faizal Abu Bakar Kie Kyon Huang Halimah Alias Bernard K B Lee Mohd Noor Mat Isa Shuhaila Mat-Sharani Suhaila Sulaiman Lih Jinq Tay Radziah Zolkefli Yusuf Muhammad Noor Douglas Sie Nguong Law Siti Hamidah Abdul Rahman Rosli Md-Illias Farah Diba Abu Bakar Nazalan Najimudin Abdul Munir Abdul Murad Nor Muhammad Mahadi |
author_facet |
Mohd Firdaus-Raih Noor Haza Fazlin Hashim Izwan Bharudin Mohd Faizal Abu Bakar Kie Kyon Huang Halimah Alias Bernard K B Lee Mohd Noor Mat Isa Shuhaila Mat-Sharani Suhaila Sulaiman Lih Jinq Tay Radziah Zolkefli Yusuf Muhammad Noor Douglas Sie Nguong Law Siti Hamidah Abdul Rahman Rosli Md-Illias Farah Diba Abu Bakar Nazalan Najimudin Abdul Munir Abdul Murad Nor Muhammad Mahadi |
author_sort |
Mohd Firdaus-Raih |
title |
The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
title_short |
The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
title_full |
The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
title_fullStr |
The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
title_full_unstemmed |
The Glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
title_sort |
glaciozyma antarctica genome reveals an array of systems that provide sustained responses towards temperature variations in a persistently cold habitat. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pone.0189947 https://doaj.org/article/c535eb7e07ea42f887282ca670758a77 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
PLoS ONE, Vol 13, Iss 1, p e0189947 (2018) |
op_relation |
http://europepmc.org/articles/PMC5791967?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0189947 https://doaj.org/article/c535eb7e07ea42f887282ca670758a77 |
op_doi |
https://doi.org/10.1371/journal.pone.0189947 |
container_title |
PLOS ONE |
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13 |
container_issue |
1 |
container_start_page |
e0189947 |
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1766072950733144064 |