Arginase activity in the blood of patients with visceral leishmaniasis and HIV infection.
Visceral leishmaniasis is a parasitic disease associated with high mortality. The most important foci of visceral leishmaniasis in Ethiopia are in the Northwest and are predominantly associated with high rates of HIV co-infection. Co-infection of visceral leishmaniasis patients with HIV results in h...
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ftdoajarticles:oai:doaj.org/article:c517bfa47b964ef9aef2a757bb0024e5 2023-05-15T15:11:22+02:00 Arginase activity in the blood of patients with visceral leishmaniasis and HIV infection. Yegnasew Takele Tamrat Abebe Teklu Weldegebreal Asrat Hailu Workagegnehu Hailu Zewdu Hurissa Jemal Ali Ermiyas Diro Yifru Sisay Tom Cloke Manuel Modolell Markus Munder Fabienne Tacchini-Cottier Ingrid Müller Pascale Kropf 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001977 https://doaj.org/article/c517bfa47b964ef9aef2a757bb0024e5 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3547864?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001977 https://doaj.org/article/c517bfa47b964ef9aef2a757bb0024e5 PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e1977 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001977 2022-12-31T13:27:19Z Visceral leishmaniasis is a parasitic disease associated with high mortality. The most important foci of visceral leishmaniasis in Ethiopia are in the Northwest and are predominantly associated with high rates of HIV co-infection. Co-infection of visceral leishmaniasis patients with HIV results in higher mortality, treatment failure and relapse. We have previously shown that arginase, an enzyme associated with immunosuppression, was increased in patients with visceral leishmaniasis and in HIV seropositive patients; further our results showed that high arginase activity is a marker of disease severity. Here, we tested the hypothesis that increased arginase activities associated with visceral leishmaniasis and HIV infections synergize in patients co-infected with both pathogens.We recruited a cohort of patients with visceral leishmaniasis and a cohort of patients with visceral leishmaniasis and HIV infection from Gondar, Northwest Ethiopia, and recorded and compared their clinical data. Further, we measured the levels of arginase activity in the blood of these patients and identified the phenotype of arginase-expressing cells. Our results show that CD4(+) T cell counts were significantly lower and the parasite load in the spleen was significantly higher in co-infected patients. Moreover, our results demonstrate that arginase activity was significantly higher in peripheral blood mononuclear cells and plasma of co-infected patients. Finally, we identified the cells-expressing arginase in the PBMCs as low-density granulocytes.Our results suggest that increased arginase might contribute to the poor disease outcome characteristic of patients with visceral leishmaniasis and HIV co-infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 1 e1977 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Yegnasew Takele Tamrat Abebe Teklu Weldegebreal Asrat Hailu Workagegnehu Hailu Zewdu Hurissa Jemal Ali Ermiyas Diro Yifru Sisay Tom Cloke Manuel Modolell Markus Munder Fabienne Tacchini-Cottier Ingrid Müller Pascale Kropf Arginase activity in the blood of patients with visceral leishmaniasis and HIV infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Visceral leishmaniasis is a parasitic disease associated with high mortality. The most important foci of visceral leishmaniasis in Ethiopia are in the Northwest and are predominantly associated with high rates of HIV co-infection. Co-infection of visceral leishmaniasis patients with HIV results in higher mortality, treatment failure and relapse. We have previously shown that arginase, an enzyme associated with immunosuppression, was increased in patients with visceral leishmaniasis and in HIV seropositive patients; further our results showed that high arginase activity is a marker of disease severity. Here, we tested the hypothesis that increased arginase activities associated with visceral leishmaniasis and HIV infections synergize in patients co-infected with both pathogens.We recruited a cohort of patients with visceral leishmaniasis and a cohort of patients with visceral leishmaniasis and HIV infection from Gondar, Northwest Ethiopia, and recorded and compared their clinical data. Further, we measured the levels of arginase activity in the blood of these patients and identified the phenotype of arginase-expressing cells. Our results show that CD4(+) T cell counts were significantly lower and the parasite load in the spleen was significantly higher in co-infected patients. Moreover, our results demonstrate that arginase activity was significantly higher in peripheral blood mononuclear cells and plasma of co-infected patients. Finally, we identified the cells-expressing arginase in the PBMCs as low-density granulocytes.Our results suggest that increased arginase might contribute to the poor disease outcome characteristic of patients with visceral leishmaniasis and HIV co-infection. |
format |
Article in Journal/Newspaper |
author |
Yegnasew Takele Tamrat Abebe Teklu Weldegebreal Asrat Hailu Workagegnehu Hailu Zewdu Hurissa Jemal Ali Ermiyas Diro Yifru Sisay Tom Cloke Manuel Modolell Markus Munder Fabienne Tacchini-Cottier Ingrid Müller Pascale Kropf |
author_facet |
Yegnasew Takele Tamrat Abebe Teklu Weldegebreal Asrat Hailu Workagegnehu Hailu Zewdu Hurissa Jemal Ali Ermiyas Diro Yifru Sisay Tom Cloke Manuel Modolell Markus Munder Fabienne Tacchini-Cottier Ingrid Müller Pascale Kropf |
author_sort |
Yegnasew Takele |
title |
Arginase activity in the blood of patients with visceral leishmaniasis and HIV infection. |
title_short |
Arginase activity in the blood of patients with visceral leishmaniasis and HIV infection. |
title_full |
Arginase activity in the blood of patients with visceral leishmaniasis and HIV infection. |
title_fullStr |
Arginase activity in the blood of patients with visceral leishmaniasis and HIV infection. |
title_full_unstemmed |
Arginase activity in the blood of patients with visceral leishmaniasis and HIV infection. |
title_sort |
arginase activity in the blood of patients with visceral leishmaniasis and hiv infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0001977 https://doaj.org/article/c517bfa47b964ef9aef2a757bb0024e5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e1977 (2013) |
op_relation |
http://europepmc.org/articles/PMC3547864?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001977 https://doaj.org/article/c517bfa47b964ef9aef2a757bb0024e5 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001977 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
1 |
container_start_page |
e1977 |
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1766342230695477248 |